SparQ™ Cumate Switch Starter Pack
- Robust—increase expression up to 32-fold
- Adjustable—tune expression levels by titrating the amount of cumate
- Reversible—turn expression on, then off, then on again
- Versatile—choose from all-in-one formats that co-express CymR and your gene-of-interest, or two-vector systems where CymR is expressed from a different plasmid
- Powerful—suitable for in vivo applications
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| QM600A-1 | Cumate Starter Pack: Your choice of inducible vector below, + CymR-T2A-Puro (packaged virus, QM200VA-1) and Cumate solution (1000x, QM100A-1). Indicate your inducible lentivector catalog # when placing Order. | 1 Kit | $1195 |
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Overview
Overview
Get started with the SparQ™ Cumate Switch System
We’ve made it easy to get started with the SparQ™ Cumate Switch System, with the SparQ Cumate Switch Starter Pack. The Starter Pack includes all three components of the SparQ Cumate Switch System so you can quickly get cumate-inducible expression of your gene- or miRNA-of-interest into your lab.
- Robust—increase expression up to 32-fold
- Adjustable—tune expression levels by titrating the amount of cumate
- Reversible—turn expression on, then off, then on again
- Versatile—choose from all-in-one formats that co-express CymR and your gene-of-interest, or two-vector systems where CymR is expressed from a different plasmid
- Powerful—suitable for in vivo applications
With SBI’s SparQ™ Cumate Switch System, you can get inducible gene expression in mammalian cells through the binding of cumate, a non-toxic small molecule, to CymR. Expression levels of your gene-of-interest are tightly controlled and increase with increasing cumate concentration until maximum induction is reached—see as much as a 32-fold increase in gene expression. Even better, induction is reversible, so you can turn expression levels on and off. Delivering negligible background expression in the absence of cumate, the SparQ System is an excellent choice for achieving controlled levels of gene expression.
What’s included in the SparQ Cumate Switch Starter Pack?
The Starter Pack includes:
- CymR expression lentivector pCDH-EF1α-CymR-T2A-Puro as pre-packaged virus (# QM200VA-2)
- Cumate Solution (1,000x; # QM100A-1)
- And any one of our two-component SparQ Cloning and Expression Lentivectors (the All-in-one SparQ Lentivectors Cat.# QM800A-1 and QM812B-1 are not eligible to be included in the SparQ Cumate Switch Starter Pack)
- pCDH-CuO-MCS (# QM500A-1)
- pCDH-CuO-MCS-EF1α-GFP (# QM511B-1)
- pCDH-CuO-MCS-EF1α-RFP (# QM512B-1)
- pCDH-CuO-MCS-EF1α-GFP+Puro (# QM513B-1)
- pCDH-CuO-MCS-EF1α-RFP+Puro (# QM516B-2)
- pCDH-CuO-MCS-T2A-GFP (# QM521A-1)
- pCDH-CuO-MCS-T2A-RFP (# QM522A-1)
- pCDH-CuO-MCS-IRES-GFP (# QM530A-2)
- pCDH-CuO-MCS-IRES-RFP (# QM531A-2)
References
How It Works
How It Works
Tightly-controlled, inducible gene expression
SBI’s SparQ Cumate Switch System delivers robust, titratable gene expression with low background through three components:
- Cumate, a non-toxic, small-molecule inducer
- CymR, a repressor that binds to cumate operator sequences in the absence of cumate
- SparQ Lentivector that contains an MCS to clone-in your gene-of-interest, the cumate inducible promoter with cumate operator sequences (CuO) upstream of the MCS, and one or more markers
Supporting Data
Supporting Data
Tight expression control with low background with the SparQ Cumate Switch System
Figure 1. Get lower background and higher induction with the SparQ Cumate Switch System than other inducible systems.
Figure 2. Gene expression with the SparQ Cumate SwitchSystem can be turned on and off, then on again.
Figure 3. Gene expression with the SparQ Cumate Switch System is titratable, with increasing amounts of cumate inducing a linear increase in gene expression.
Figure 4. With the SparQ System, gene expression can also be titrated by increasing the amount of transduced SparQ lentivirus, even up to 30 MOI.
FAQs
Resources
Citations
Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| QM600A-1 | Cumate Starter Pack: Your choice of inducible vector below, + CymR-T2A-Puro (packaged virus, QM200VA-1) and Cumate solution (1000x, QM100A-1). Indicate your inducible lentivector catalog # when placing Order. | 1 Kit | $1195 |
|
||||
Overview
Overview
Get started with the SparQ™ Cumate Switch System
We’ve made it easy to get started with the SparQ™ Cumate Switch System, with the SparQ Cumate Switch Starter Pack. The Starter Pack includes all three components of the SparQ Cumate Switch System so you can quickly get cumate-inducible expression of your gene- or miRNA-of-interest into your lab.
- Robust—increase expression up to 32-fold
- Adjustable—tune expression levels by titrating the amount of cumate
- Reversible—turn expression on, then off, then on again
- Versatile—choose from all-in-one formats that co-express CymR and your gene-of-interest, or two-vector systems where CymR is expressed from a different plasmid
- Powerful—suitable for in vivo applications
With SBI’s SparQ™ Cumate Switch System, you can get inducible gene expression in mammalian cells through the binding of cumate, a non-toxic small molecule, to CymR. Expression levels of your gene-of-interest are tightly controlled and increase with increasing cumate concentration until maximum induction is reached—see as much as a 32-fold increase in gene expression. Even better, induction is reversible, so you can turn expression levels on and off. Delivering negligible background expression in the absence of cumate, the SparQ System is an excellent choice for achieving controlled levels of gene expression.
What’s included in the SparQ Cumate Switch Starter Pack?
The Starter Pack includes:
- CymR expression lentivector pCDH-EF1α-CymR-T2A-Puro as pre-packaged virus (# QM200VA-2)
- Cumate Solution (1,000x; # QM100A-1)
- And any one of our two-component SparQ Cloning and Expression Lentivectors (the All-in-one SparQ Lentivectors Cat.# QM800A-1 and QM812B-1 are not eligible to be included in the SparQ Cumate Switch Starter Pack)
- pCDH-CuO-MCS (# QM500A-1)
- pCDH-CuO-MCS-EF1α-GFP (# QM511B-1)
- pCDH-CuO-MCS-EF1α-RFP (# QM512B-1)
- pCDH-CuO-MCS-EF1α-GFP+Puro (# QM513B-1)
- pCDH-CuO-MCS-EF1α-RFP+Puro (# QM516B-2)
- pCDH-CuO-MCS-T2A-GFP (# QM521A-1)
- pCDH-CuO-MCS-T2A-RFP (# QM522A-1)
- pCDH-CuO-MCS-IRES-GFP (# QM530A-2)
- pCDH-CuO-MCS-IRES-RFP (# QM531A-2)
References
How It Works
How It Works
Tightly-controlled, inducible gene expression
SBI’s SparQ Cumate Switch System delivers robust, titratable gene expression with low background through three components:
- Cumate, a non-toxic, small-molecule inducer
- CymR, a repressor that binds to cumate operator sequences in the absence of cumate
- SparQ Lentivector that contains an MCS to clone-in your gene-of-interest, the cumate inducible promoter with cumate operator sequences (CuO) upstream of the MCS, and one or more markers
Supporting Data
Supporting Data
Tight expression control with low background with the SparQ Cumate Switch SystemFigure 1. Get lower background and higher induction with the SparQ Cumate Switch System than other inducible systems.
Figure 2. Gene expression with the SparQ Cumate SwitchSystem can be turned on and off, then on again.
Figure 3. Gene expression with the SparQ Cumate Switch System is titratable, with increasing amounts of cumate inducing a linear increase in gene expression.
Figure 4. With the SparQ System, gene expression can also be titrated by increasing the amount of transduced SparQ lentivirus, even up to 30 MOI.