mRNAExpress™ hMyoD Transcript

Save time and deliver human hMyoD mRNA to cells for differentiation or other studies using this ready-to-transfect, pre-made mRNAExpress transcript

Catalog Number
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mRNAExpress hMyoD transcript

10 µg
$ 213

mRNAExpress hMyoD transcript

20 µg
$ 306
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Save time by delivering pre-made mRNAs

Save time when you want instant expression after transfection and use one of SBI’s pre-made, ready-to-transfect mRNAs. Like all of our pre-made mRNAs, the mRNAExpress™ hMyoD Transcript contains a number of different features to enhance performance:

  • An anti-reverse cap analog (ARCA) to increase translation efficiency
  • Modified nucleotides (5-Methylcytidine-5′-Triphosphate and Pseudouridine-5′-Triphosphate) and a poly-A tail to enhance stability and reduce host cell immune response
  • Removal of the 5′ triphosphates at the end of the RNA to further reduce innate immune responses in mammalian cells

Generate induced pluripotent stem cells (iPSCs) with mRNAExpress transcripts:

Cat.# mRNAExpress Transcript
MR100A-1 mRNAExpress hOct4 transcript
MR200A-1 mRNAExpress hSox2 transcript
MR300A-1 mRNAExpress hKlf4 transcript
MR400A-1 mRNAExpress hcMyc transcript
MR500A-1 mRNAExpress hLin28 transcript
MR600A-1 mRNAExpress hNanog transcript
MR700A-1 mRNAExpress GFP transcript (positive control)

Supporting Data

The mRNAExpress hMyoD transcript drives transdifferentiation

The mRNAExpress hMyoD transcript drives transdifferentiation into myocytes

Figure 1. The mRNAExpress hMyoD transcript drives transdifferentiation into myocytes. Human foreskin fibroblasts were transfected with 1 µg of the mRNAExpress hMyoD Transcript per 24-well plate three times on three consecutive days (transfection was enhanced with RNAfection Transfection Reagent (Cat.# MR750A-1)). After an additional three days of transdifferentiation, the cells were immunostained for Desmin, a myogenic marker, to visualize myotube formation, and nuclei imaged with DAPI. The mRNAExpress hMyoD Transcript successfully transdifferentiated fibroblasts into myocytes.