Reversible transgenesis that leaves no trace behind
One of the advantages of the PiggyBac Transposon System* is the reversibility of the integration event. With the Excision-only PiggyBac Transposase, delivered by transient transfection of the Exicison-only PiggyBac Transposase Expression Vector, you can remove any DNA integrated into the genome using the PiggyBac Transposon System. After removal of the PiggyBac insert, the genomic DNA is restored to the original sequence with no residual PiggyBac sequences left behind, resulting in a truly footprint-free removal.
Both the Excision-only PiggyBac Transposase and the Super PiggyBac Transposase (Cat.# PB210PA-1) recognize transposon-specific inverted terminal repeats (ITRs). While the Super PiggyBac Transposase has both excision and integration activities that enable the enzyme to mediate removal of the ITRs and intervening DNA from one location (the vector) and insert that DNA fragment into another location (the genome), the Excision-only PiggyBac Transposase only has the excision activity. Thus, the Excision-only PiggyBac Transposase can be used to remove DNA segments from the genome that are bordered by the PiggyBac transposon ITRs.
With the PiggyBac Transposon System, you can:
- Make transgenic cell lines with a single transfection
- Integrate multiple PiggyBac Vectors in a single transfection
- Insert an expression cassette into human, mouse, and rat cells
- Deliver virtually any-sized DNA insert, from 10 – 100 kb
- Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
- Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.
For end user license information, see the following:
* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.