Mouse RORγt Promoter GreenFire Lentivector
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| TCL800A-1 | Mouse RORyt Promoter GreenFire lentivector plasmid (with Puro marker) | 10 µg | $709 |
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Overview
Overview
Monitor the balance of T cell dynamics in autoimmunity, inflammation, and cancer
Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines. With the Mouse RORγt Promoter GreenFire Lentivector you can combine SBI’s well-regarded and high quality lentivector technology with a RORγt promoter-reporter architecture that enables easy and quantitative monitoring of RORγt expression. The RORγt promoter drives coordinated expression of GFP and luciferase reporters.
The promoter sequences and design are based upon Dang, et al.1, Lazarevic, et al.2, and Ruan, et al3.
References
How It Works
Supporting Data
Supporting Data
Luciferase activation in response to RORγt transcriptional activators
Figure 1. SBI’s GreenFire Lentivector reporters for mFoxp3, mRORγt, and mIL-17 show strong luciferase activity in response to known transcriptional activators. METHODS: Human Jurkat T cells were transduced with lentivirus for the Foxp3, RORγt and IL-17 promoter reporters. Transcription activation was tested using stimulation through the addition of with PMA (5 ng/ml) and Ionomycin (500 ng/ml). Cells were harvested after 24 hours and luciferase activity measured for transcription activation responses.
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Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| TCL800A-1 | Mouse RORyt Promoter GreenFire lentivector plasmid (with Puro marker) | 10 µg | $709 |
|
||||
Overview
Overview
Monitor the balance of T cell dynamics in autoimmunity, inflammation, and cancer
Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines. With the Mouse RORγt Promoter GreenFire Lentivector you can combine SBI’s well-regarded and high quality lentivector technology with a RORγt promoter-reporter architecture that enables easy and quantitative monitoring of RORγt expression. The RORγt promoter drives coordinated expression of GFP and luciferase reporters.
The promoter sequences and design are based upon Dang, et al.1, Lazarevic, et al.2, and Ruan, et al3.
References
How It Works
Supporting Data
Supporting Data
Luciferase activation in response to RORγt transcriptional activators
Figure 1. SBI’s GreenFire Lentivector reporters for mFoxp3, mRORγt, and mIL-17 show strong luciferase activity in response to known transcriptional activators. METHODS: Human Jurkat T cells were transduced with lentivirus for the Foxp3, RORγt and IL-17 promoter reporters. Transcription activation was tested using stimulation through the addition of with PMA (5 ng/ml) and Ionomycin (500 ng/ml). Cells were harvested after 24 hours and luciferase activity measured for transcription activation responses.