pGreenFire1-mCMV (EF1α-puro) Negative Control Lentivector
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| TR010PA-P | pGreenFire1-mCMV Plasmid (pTRH1 mCMV dscGFP T2A Fluc, negative control)+ EF1α-Puro | 10 µg | $594 |
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| TR010VA-P | pGreenFire1-mCMV Virus (pTRH1 mCMV dscGFP T2A Fluc) + EF1α-Puro | >2 x 10^6 IFUs | $747 |
|
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Overview
Overview
Supporting your studies with ready-to-go controls
No need to make a negative control for your pGreenFire projects—SBI’s already built one for you. With the pGreenFire1-mCMV (EF1α-puro) Negative Control Lentivector, the GreenFire cassette is driven by a minimal CMV promoter with destabilized copepod GFP (dscGFP, 2-hour half-life) and luciferase co-expression mediated by a T2A element. The minimal CMV promoter delivers negligible expression, and this configuration provides a control for background levels of GFP and luciferase expression in the absence of enhancer elements, such as the ones used in our Signal Transduction Pathway Reporters/Transcriptional Response Element Reporters.
To simplify cell line construction, this pGreenFire1-mCMV (EF1α-puro) Negative Control Lentivector comes with a constitutively expressed puromycin marker expressed from the Ef1α promoter. Or choose an EF1α-neo (Cat.# TR010PA-N), or no marker at all (Cat.# TR010PA-1). All versions of this lentivector are available as plasmid or pre-packaged virus.
References
How It Works
Supporting Data
Supporting Data
See our transcriptional response element reporters in action
Monitor oncogenic pathway reporters
Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis
General pGreenFire data examples
Monitoring NF-κB transactivation
FAQs
Resources
Citations
Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| TR010PA-P | pGreenFire1-mCMV Plasmid (pTRH1 mCMV dscGFP T2A Fluc, negative control)+ EF1α-Puro | 10 µg | $594 |
|
||||
| TR010VA-P | pGreenFire1-mCMV Virus (pTRH1 mCMV dscGFP T2A Fluc) + EF1α-Puro | >2 x 10^6 IFUs | $747 |
|
||||
Overview
Overview
Supporting your studies with ready-to-go controls
No need to make a negative control for your pGreenFire projects—SBI’s already built one for you. With the pGreenFire1-mCMV (EF1α-puro) Negative Control Lentivector, the GreenFire cassette is driven by a minimal CMV promoter with destabilized copepod GFP (dscGFP, 2-hour half-life) and luciferase co-expression mediated by a T2A element. The minimal CMV promoter delivers negligible expression, and this configuration provides a control for background levels of GFP and luciferase expression in the absence of enhancer elements, such as the ones used in our Signal Transduction Pathway Reporters/Transcriptional Response Element Reporters.
To simplify cell line construction, this pGreenFire1-mCMV (EF1α-puro) Negative Control Lentivector comes with a constitutively expressed puromycin marker expressed from the Ef1α promoter. Or choose an EF1α-neo (Cat.# TR010PA-N), or no marker at all (Cat.# TR010PA-1). All versions of this lentivector are available as plasmid or pre-packaged virus.
References
How It Works
Supporting Data
Supporting Data
See our transcriptional response element reporters in action
Monitor oncogenic pathway reporters
Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis
General pGreenFire data examples
Monitoring NF-κB transactivation