pMSCV-Human Foxp3-EF1α-GFP-T2A-Puro for Foxp3 Overexpression
- Academic Customers—End-User License Information
- Commercial Customers—End-User License Information
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
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TCL210C-1 | MSCV-Human Foxp3-EF1α-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant) | 5 x 10^6 Cells/mL | $1596 |
This item has been discontinued and is no longer available. Please email customer service for assistance: info@systembio.com |
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TCL200PB-1 | pMSCV-Human Foxp3-EF1α-GFP-T2A-Puro piggyBac transposon plasmid | 10 µg | $681 |
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TCL200A-1 | pMSCV-Human Foxp3-EF1α-GFP-T2A-Puro lentivector plasmid | 10 µg | $657 |
|
Overview
Overview
Study the balance of T cell dynamics in autoimmunity, inflammation, and cancer
Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines. With pMSCV-Human Foxp3-EF1α-GFP-T2A-Puro for Foxp3 Overexpression, you can choose between three different ways to study Foxp3 overexpression from the MSCV promoter for strong expression in hematopoietic and stem cells:
Lentivector-based for transfection-resistant cell lines
Create stable Foxp3-overexpressing cell lines by integrating the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette into the genome using SBI’s highly regarded and reliable lentivector technology. With our lentivector technology, you can take advantage of our high-titer lentivector packaging system to efficiently deliver your Foxp3 overexpression cassette to cells using transduction—great for transfection-resistant cell lines.
PiggyBac Transposon-based* for infection-resistant cell lines
Create stable Foxp3-overexpressing cell lines by integrating the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette into the genome with SBI’s easy and consistent PiggyBac Transposon System. With our PiggyBac Transposon System, you get easy and consistent transgenesis for Foxp3 overexpression in infection-resistant cell lines.Pre-built Jurkat cell line to save time and labor
Quickly get your Treg studies off the ground with our pre-built Jurkat cell line that already has the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette integrated into the genome. We transduced Jurkat cells with the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette and have validated Foxp3 overexpression, so you don’t have to—just culture cells and start your studies.
*Customer Agreements
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.
For end user license information, see the following:
* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.
References
How It Works
How It Works
A quick introduction to SBI’s lentivector packaging and PiggyBac Transposon technologies
Start producing lentivirus
The PiggyBac Transposon System’s Cut-and-Paste Mechanism
The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.
Supporting Data
FAQs
Resources
Citations
Related Products
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
TCL210C-1 | MSCV-Human Foxp3-EF1α-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant) | 5 x 10^6 Cells/mL | $1596 |
This item has been discontinued and is no longer available. Please email customer service for assistance: info@systembio.com |
||||
TCL200PB-1 | pMSCV-Human Foxp3-EF1α-GFP-T2A-Puro piggyBac transposon plasmid | 10 µg | $681 |
|
||||
TCL200A-1 | pMSCV-Human Foxp3-EF1α-GFP-T2A-Puro lentivector plasmid | 10 µg | $657 |
|
Overview
Overview
Study the balance of T cell dynamics in autoimmunity, inflammation, and cancer
Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines. With pMSCV-Human Foxp3-EF1α-GFP-T2A-Puro for Foxp3 Overexpression, you can choose between three different ways to study Foxp3 overexpression from the MSCV promoter for strong expression in hematopoietic and stem cells:
Lentivector-based for transfection-resistant cell lines
Create stable Foxp3-overexpressing cell lines by integrating the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette into the genome using SBI’s highly regarded and reliable lentivector technology. With our lentivector technology, you can take advantage of our high-titer lentivector packaging system to efficiently deliver your Foxp3 overexpression cassette to cells using transduction—great for transfection-resistant cell lines.
PiggyBac Transposon-based* for infection-resistant cell lines
Create stable Foxp3-overexpressing cell lines by integrating the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette into the genome with SBI’s easy and consistent PiggyBac Transposon System. With our PiggyBac Transposon System, you get easy and consistent transgenesis for Foxp3 overexpression in infection-resistant cell lines.Pre-built Jurkat cell line to save time and labor
Quickly get your Treg studies off the ground with our pre-built Jurkat cell line that already has the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette integrated into the genome. We transduced Jurkat cells with the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette and have validated Foxp3 overexpression, so you don’t have to—just culture cells and start your studies.
*Customer Agreements
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.
For end user license information, see the following:
* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.
References
How It Works
How It Works
A quick introduction to SBI’s lentivector packaging and PiggyBac Transposon technologies
Start producing lentivirus
The PiggyBac Transposon System’s Cut-and-Paste Mechanism
The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.