pSIH1-H1-siLuc-copGFP Positive Transduction Control

Overview

Gain the confidence to move forward with informative controls While lentiviral transduction can be a powerful and effective method for delivering expression cassettes to target cells, enabling stable expression of genes, miRNAs, shRNAs, etc., some cell lines are transduced less efficiently than others. To help with lentiviral transduction optimization and troubleshooting, SBI offers a series of positive transduction controls that come as pre-packaged, ready-to-transduce lentiviral particles as well as ready-to-transfect and package lentivector plasmids. The pSIH1-H1-siLuc-copGFP Positive Transduction Control enables quantitation of HIV-based lentiviral transduction efficiency using the bright copGFP protein.

• Easily visualize and quantify transduction efficiency with copGFP
• Optimize transduction conditions for your cell line
• Test the sensitivity of target cells to HIV-based lentiviral transduction

pSIH1-H1-siLuc-copGFP expresses the full-length copGFP gene with human-optimized codons from the CMV promoter for high levels of expression in mammalian cells. The copGFP marker is a novel natural green monomeric GFP-like protein from copepods (Pontellina sp.). The copGFP protein is a non-toxic, non-aggregating protein with fast protein maturation and high stability at a wide range of pH (pH 4 – 12). copGFP does not require any additional cofactors or substrates and has very bright fluorescence that exceeds at least 1.3 times the brightness of EGFP, the widely used Aequorea victoria GFP mutant. The copGFP protein emits green fluorescence with the following characteristics:

• Maximum emission wavelength: 502 nm
• Maximum excitation wavelength: 482 nm
• Quantum yield: 0.6
• Extinction coefficient: 70,000 M^-1 cm^-1