QuantiMir™ Kit
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
RA420A-1 | QuantiMir™ Kit | 20 Reactions | $609 |
|
Overview
Overview
Get your miRNA ready for qPCR profiling
When you’d like to profile miRNA with qPCR, SBI’s QuantiMir™ Kit can get your samples ready. By efficiently converting all of your miRNA into cDNA, you can get accurate, sensitive, and quantitative qPCR data on the miRs in your sample. Great for understanding differential miRNA expression, simultaneously profiling siRNA and mRNA, and more.
- Simple and robust procedure
- Rapidly and efficiently convert all small RNAs into cDNAs for qPCR
- Suitable for high-throughput screening of clinical samples
- Sensitive and accurate (see the data in the Supporting Data section below)
- Versatile—design your own miRNA assays
Catalog # | Amplified small RNA | Species compatibility |
---|---|---|
RA420A-hU6 | Human U6 | Human |
RA420A-miR-16 | miR-16 | Human, Mouse |
RA420A-mU6 | Mouse U6 | Mouse |
RA420A-RNU1 | RNU1 (U1) | Human, Mouse, Rat, Monkey |
RA420A-RNU43 | RNU43 | Human, Mouse, Rat |
RA420A-rU6 | Rat U6 | Rat |
References
How It Works
How It Works
The QuantiMir Kit enables robust miRNA quantitation through a simple and efficient workflow
Highly efficient poly-A tailing and reverse transcription in a single reaction tube provides uniform cDNA synthesis of miRNAs. The optimized reaction conditions and buffer components maximize cDNA yield when starting with several micrograms down to picograms of input total RNA. The universal 3′ tag sequence incorporated during reverse transcription enables easily scalable and accurate miRNA expression analysis by qPCR—profile thousands of different miRNAs from a single reverse transcription reaction.
- Tag all small RNAs with a poly-A tail
- Anneal an oligo-dT adaptor
- Reverse transcribe to create first-strand cDNA
The result is a cDNA pool of anchor-tailed miRNAs that are ready for qPCR.
Create custom miRNA assays
To design your own miRNA assays, simply synthesize an oligo using the sequence of the mature miRNA you’d like to profile as the forward primer in your miRNA qPCR assay, and use with the universal reverse primer included in the QuantiMir Kit.
Supporting Data
FAQs
Resources
Citations
Related Products
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
RA420A-1 | QuantiMir™ Kit | 20 Reactions | $609 |
|
Overview
Overview
Get your miRNA ready for qPCR profiling
When you’d like to profile miRNA with qPCR, SBI’s QuantiMir™ Kit can get your samples ready. By efficiently converting all of your miRNA into cDNA, you can get accurate, sensitive, and quantitative qPCR data on the miRs in your sample. Great for understanding differential miRNA expression, simultaneously profiling siRNA and mRNA, and more.
- Simple and robust procedure
- Rapidly and efficiently convert all small RNAs into cDNAs for qPCR
- Suitable for high-throughput screening of clinical samples
- Sensitive and accurate (see the data in the Supporting Data section below)
- Versatile—design your own miRNA assays
Catalog # | Amplified small RNA | Species compatibility |
---|---|---|
RA420A-hU6 | Human U6 | Human |
RA420A-miR-16 | miR-16 | Human, Mouse |
RA420A-mU6 | Mouse U6 | Mouse |
RA420A-RNU1 | RNU1 (U1) | Human, Mouse, Rat, Monkey |
RA420A-RNU43 | RNU43 | Human, Mouse, Rat |
RA420A-rU6 | Rat U6 | Rat |
References
How It Works
How It Works
The QuantiMir Kit enables robust miRNA quantitation through a simple and efficient workflow
Highly efficient poly-A tailing and reverse transcription in a single reaction tube provides uniform cDNA synthesis of miRNAs. The optimized reaction conditions and buffer components maximize cDNA yield when starting with several micrograms down to picograms of input total RNA. The universal 3′ tag sequence incorporated during reverse transcription enables easily scalable and accurate miRNA expression analysis by qPCR—profile thousands of different miRNAs from a single reverse transcription reaction.
- Tag all small RNAs with a poly-A tail
- Anneal an oligo-dT adaptor
- Reverse transcribe to create first-strand cDNA
The result is a cDNA pool of anchor-tailed miRNAs that are ready for qPCR.
Create custom miRNA assays
To design your own miRNA assays, simply synthesize an oligo using the sequence of the mature miRNA you’d like to profile as the forward primer in your miRNA qPCR assay, and use with the universal reverse primer included in the QuantiMir Kit.