pCMV-CRE, Cre Recombinase Expression Plasmid
Transiently express Cre recombinase to remove selection cassettes from your genome engineering projects—use with HR Targeting Vectors that contain LoxP sites
Get rid of selection cassettes and other unwanted sequences in your genome engineering projects
With the Cre-LoxP site-specific recombination system used in most of our HR Targeting Vectors, you can get rid of extra sequences, such as selection cassettes, by transiently expressing the Cre recombinase from the pCMV-CRE Cre Recombinase Expression Plasmid.
How It Works
Removing a selection cassette with Cre recombinase
Cre recombinase catalyzes site-specific recombination between two LoxP sites. After recombination occurs, the sequences between the two LoxP sites are excised from the genome and a single LoxP site remains (Figure 1). Thus, with our HR Targeting Vectors that contain two LoxP sites, all the vector sequences that lie between the LoxP sites can be removed through transient expression of the Cre recombinase (Figure 1).