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SparQ™ Cumate Switch Starter Pack

Get started with the SparQ Cumate Switch System with this kit that includes a cloning and expression lentivector, CymR lentivector, and cumate solution

  • Robust—increase expression up to 32-fold
  • Adjustable—tune expression levels by titrating the amount of cumate
  • Reversible—turn expression on, then off, then on again
  • Versatile—choose from all-in-one formats that co-express CymR and your gene-of-interest, or two-vector systems where CymR is expressed from a different plasmid
  • Powerful—suitable for in vivo applications
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Cumate Starter Pack: Your choice of inducible vector below, + CymR-T2A-Puro (packaged virus, QM200VA-1) and Cumate solution (1000x, QM100A-1). Indicate your inducible lentivector catalog # when placing Order.
1 Kit
$ 1105


Get started with the SparQ™ Cumate Switch System

We’ve made it easy to get started with the SparQ™ Cumate Switch System, with the SparQ Cumate Switch Starter Pack. The Starter Pack includes all three components of the SparQ Cumate Switch System so you can quickly get cumate-inducible expression of your gene- or miRNA-of-interest into your lab.

  • Robust—increase expression up to 32-fold
  • Adjustable—tune expression levels by titrating the amount of cumate
  • Reversible—turn expression on, then off, then on again
  • Versatile—choose from all-in-one formats that co-express CymR and your gene-of-interest, or two-vector systems where CymR is expressed from a different plasmid
  • Powerful—suitable for in vivo applications

With SBI’s SparQ™ Cumate Switch System, you can get inducible gene expression in mammalian cells through the binding of cumate, a non-toxic small molecule, to CymR. Expression levels of your gene-of-interest are tightly controlled and increase with increasing cumate concentration until maximum induction is reached—see as much as a 32-fold increase in gene expression. Even better, induction is reversible, so you can turn expression levels on and off. Delivering negligible background expression in the absence of cumate, the SparQ System is an excellent choice for achieving controlled levels of gene expression.

What’s included in the SparQ Cumate Switch Starter Pack?

The Starter Pack includes:

  • CymR expression lentivector pCDH-EF1α-CymR-T2A-Puro as pre-packaged virus (# QM200VA-2)
  • Cumate Solution (1,000x; # QM100A-1)
  • And any one of our two-component SparQ Cloning and Expression Lentivectors (the All-in-one SparQ Lentivectors Cat.# QM800A-1 and QM812B-1 are not eligible to be included in the SparQ Cumate Switch Starter Pack)

How It Works

Tightly-controlled, inducible gene expression

SBI’s SparQ Cumate Switch System delivers robust, titratable gene expression with low background through three components:

  • Cumate, a non-toxic, small-molecule inducer
  • CymR, a repressor that binds to cumate operator sequences in the absence of cumate
  • SparQ Lentivector that contains an MCS to clone-in your gene-of-interest, the cumate inducible promoter with cumate operator sequences (CuO) upstream of the MCS, and one or more markers

CymR has a high binding affinity for cumate and, as more cumate is added, fewer CymR molecules bind to the CuO sequences in the promoter resulting in increased expression. Exhibiting much lower background expression than similar systems, SBI’s cumate-inducible vectors can provide up to 32-fold induction of gene expression.

Supporting Data

Tight expression control with low background with the SparQ Cumate Switch System

Figure 1. Get lower background and higher induction with the SparQ Cumate Switch System than other inducible systems.

Figure 2. Gene expression with the SparQ Cumate SwitchSystem can be turned on and off, then on again.

Figure 3. Gene expression with the SparQ Cumate Switch System is titratable, with increasing amounts of cumate inducing a linear increase in gene expression.

Figure 4. With the SparQ System, gene expression can also be titrated by increasing the amount of transduced SparQ lentivirus, even up to 30 MOI.


  • Shinmura, K, et al. (2017) WDR62 overexpression is associated with a poor prognosis in patients with lung adenocarcinoma. Mol. Carcinog.. 2017 Aug 1; 56(8):1984-1991. PM ID: 28277612
  • Maegawa, KI, et al. (2017) The Highly Dynamic Nature of ERdj5 Is Key to Efficient Elimination of Aberrant Protein Oligomers through ER-Associated Degradation. Structure. 2017 Jun 6; 25(6):846-857.e4. PM ID: 28479060
  • Park, TS, Kim, SW & Lee, JH. (2017) Efficient transgene expression system using a cumate-inducible promoter and Cre-loxP recombination in avian cells. Asian-australas. J. Anim. Sci.. 2017 Jun 1; 30(6):886-892. PM ID: 27764912
  • Herrington, KA, et al. (2017) Spatial analysis of Cdc42 activity reveals a role for plasma membrane-associated Cdc42 in centrosome regulation. Mol. Biol. Cell. 2017 May 24;. PM ID: 28539409
  • Qi, Z, et al. (2017) An optimized, broadly applicable piggyBac transposon induction system. Nucleic Acids Res.. 2017 Apr 20; 45(7):e55. PM ID: 28082389
  • Jain, A, et al. (2017) Abl kinase regulation by BRAF/ERK and cooperation with Akt in melanoma. Oncogene. 2017 Apr 3;. PM ID: 28368422
  • O'Hara, SP, et al. (2017) ETS Proto-oncogene 1 Transcriptionally Up-regulates the Cholangiocyte Senescence-associated Protein Cyclin-dependent Kinase Inhibitor 2A. J. Biol. Chem.. 2017 Mar 24; 292(12):4833-4846. PM ID: 28184004
  • Ikushima, S & Boeke, JD. (2017) New Orthogonal Transcriptional Switches Derived from Tet Repressor Homologues for Saccharomyces cerevisiae Regulated by 2,4-Diacetylphloroglucinol and Other Ligands. ACS Synth Biol. 2017 Mar 17; 6(3):497-506. PM ID: 28005347
  • Shinmura, K, et al. (2017) Reduced expression of the DNA glycosylase gene MUTYH is associated with an increased number of somatic mutations via a reduction in the DNA repair capacity in prostate adenocarcinoma. Mol. Carcinog.. 2017 Feb 1; 56(2):781-788. PM ID: 27253753
  • Liu, L, Huang, W & Huang, JD. (2017) Synthetic circuits that process multiple light and chemical signal inputs. BMC Syst Biol. 2017 Jan 19; 11(1):5. PM ID: 28103878
  • Ando, T, et al. (2017) Ameloblastin induces tumor suppressive phenotype and enhances chemosensitivity to Dox via Src-Stat3 inactivation in osteosarcoma. Sci Rep. 2017 Jan 5; 7:40187. PM ID: 28054649
  • Choi, JS, et al. (2016) cIAPs promote the proteasomal degradation of mutant SOD1 linked to familial amyotrophic lateral sclerosis. Biochem. Biophys. Res. Commun.. 2016 Nov 18; 480(3):422-428. PM ID: 27773815
  • Barta, T, Peskova, L & Hampl, A. (2016) miRNAsong: a web-based tool for generation and testing of miRNA sponge constructs in silico. Sci Rep. 2016 Nov 18; 6:36625. PM ID: 27857164
  • Ushioda, R, et al. (2016) Redox-assisted regulation of Ca2+ homeostasis in the endoplasmic reticulum by disulfide reductase ERdj5. Proc. Natl. Acad. Sci. U.S.A.. 2016 Oct 11; 113(41):E6055-E6063. PM ID: 27694578
  • Welti, J, et al. (2016) Analytical Validation and Clinical Qualification of a New Immunohistochemical Assay for Androgen Receptor Splice Variant-7 Protein Expression in Metastatic Castration-resistant Prostate Cancer. Eur. Urol.. 2016 Oct 1; 70(4):599-608. PM ID: 27117751
  • Narumi, S, et al. (2016) SAMD9 mutations cause a novel multisystem disorder, MIRAGE syndrome, and are associated with loss of chromosome 7. Nat. Genet.. 2016 Jul 1; 48(7):792-7. PM ID: 27182967
  • Park, BO, et al. (2016) Selective novel inverse agonists for human GPR43 augment GLP-1 secretion. Eur. J. Pharmacol.. 2016 Jan 15; 771:1-9. PM ID: 26683635
  • Herrington, KA. (2016) New technologies for a better understanding of the Golgi: FLIM-FRET and click chemistry. Thesis. ;. Link: Thesis
  • Shinmura, K, et al. (2015) NEIL1 p.Gln282Stop variant is predominantly localized in the cytoplasm and exhibits reduced activity in suppressing mutations. Gene. 2015 Oct 15; 571(1):33-42. PM ID: 26095805
  • Peterson, MF, et al. (2015) Integrated systems for exosome investigation. Methods. 2015 Oct 1; 87:31-45. PM ID: 25916618

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