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EVeryRNA™ cDNA Synthesis & Pre-Amplification Kit

Get your EV RNA ready for downstream analysis with this quick and easy cDNA synthesis kit optimized for use with the EVeryRNA EV RNA Purification System.

  • Get quick (<2 hrs) and easy cDNA synthesis optimized for use with the EVeryRNA EV RNA Purification System
  • Find low abundance RNAs with the optional pre-amplification step
  • Use with most downstream applications such as PCR, qPCR and miRNA profiling
  • Buy the kit bundled with the EVeryRNA EV Purification System for added convenience
Catalog Number
Description
Size
Price
Quantity
Add to Cart
EVery200A-1
EVeryRNA™ cDNA Synthesis & Pre-Amplification Kit
20 Preps
$ 295
EVery300A-1
EVeryRNA™ EV RNA Purification System & cDNA Synthesis Kit (includes EVery100A-1 and EVery200A-1)
20 Preps
$ 675

Overview

Accelerate your EV biomarker studies

Optimized for use with the EVeryRNA EV Purification System, the EVeryRNA cDNA Synthesis & Pre-amplification Kit provides high quality exosomal cDNA for your downstream applications such as PCR, qPCR, miRNA profiling, and RNA-seq. The kit generates cDNA through poly(A) tailing of exoRNA followed by reverse transcription with MMLV Reverse Transcriptase. This method uses the template-switching oligonucleotide property of MMLV RT to generate cDNA molecules with two adaptor primers from both 5’- and 3’- ends, which can then be amplified with the included DNA Polymerase, if desired.

  • Get quick (<2 hrs) and easy cDNA synthesis optimized for use with the EVeryRNA EV RNA Purification System
  • Find low abundance RNAs with the optional pre-amplification step
  • Use with most downstream applications such as PCR, qPCR and miRNA profiling
  • Buy the kit bundled with the EVeryRNA EV Purification System for added convenience

The EVeryRNA cDNA Synthesis & Pre-amplification Kit comes with sufficient reagents to perform 20 purification reactions. EVeryRNA technology is also available bundled with SBI’s powerful EV isolation technologies as well as a cDNA Synthesis and Pre-amplification Kit (Table 1).

Table 1. EVeryRNA EV RNA Purification Products

Cat. #Product description
EVery100A-1EVeryRNA™ EV RNA Purification System
EVery106EQ-1EVeryRNA™ EV RNA Purification System with ExoQuick EV Isolation
EVery106TC-1EVeryRNA™ EV RNA Purification System with ExoQuick-TC EV Isolation
EVery110SS-1EVeryRNA™ EV RNA Purification System with SmartSEC Single
EVery200A-1EVeryRNA™ cDNA Synthesis & Pre-Amplification Kit
EVery300A-1EVeryRNA™ EV RNA Purification System & cDNA Synthesis Kit (includes EVery100A-1 and EVery200A-1)

 

 

Supporting Data

Get robust cDNA synthesis with EVeryRNA

To demonstrate the excellent RNA yields and robust cDNA synthesis obtained with EVeryRNA, we isolated EVs from 250 µL of serum using SmartSEC Single, spiked in 0.1 pmol of Cel-miR-39, and used both the EVeryRNA EV Purification System and a phenol-based kit to isolate RNA. The isolated RNA was reverse transcribed using the EVeryRNA cDNA Synthesis & Pre-amplification Kit and the copy number of Cel-miR-39 measured (Figure 1). The EVeryRNA EV Purification System delivered similar levels of Cel-miR-39 as the phenol-based method.

Figure 1. EVeryRNA is EVery bit as good as phenol.

EVeryRNA efficiently isolates mRNA

We used the EVeryRNA EV RNA Purification System and EVeryRNA cDNA Synthesis & Pre-amplification Kit to isolate mRNA and synthesize cDNA from cells overexpressing eGFP (Figure 2). Robust levels of eGFP mRNA are recovered and converted to cDNA when cells are overexpressing eGFP.

Figure 2. EVeryRNA efficiently isolates longer RNAs like mRNA.

miRNA isolated and converted to cDNA using EVeryRNA can be used for miRNA profiling

We isolated EVs from 250 µL of serum using SmartSEC Single and used the EVeryRNA EV RNA Purification System and EVeryRNA cDNA Synthesis & Pre-amplification System to isolate and reverse transcribe EV RNAs for miRNA profiling using the SeraMir Human Exosome RNA Profiling Plate. We were able to detect a number of miRNAs both with amplification (96 miRNAs) and without amplification (16 miRNAs, with 14 overlapping with the miRNAs detected with amplification, Figure 3).

Figure 3. EVeryRNA generates high-quality RNA suitable for miRNA profiling both with and without amplification.

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