Exo-FlowMag96 96-well Magnetic Plate
96-well magnetic plate for Exo-Flow IP Kits—high-throughput, plate-based immunopurification of exosomes from serum, plasma, media, and other biofluids
Overview
Simplify your bead-based immunopurifications
Validated to work with any of our Exo-Flow32 or Exo-Flow96 IP Kits, the Exo-FlowMag96 Magnetic Plate simplifies plate-based exosome isolation using magnetic beads.
Use the Exo-Flow Multifunctional Magnetic Stand with any of our Exo-Flow Capture Kits:
| Cat.# | Kit |
|---|---|
| EXOFLOW100A-1 | CD9 Exo-Flow Capture Kit |
| EXOFLOW150A-1 | Tetraspanin Exo-Flow Combo Capture Kit |
| EXOFLOW200A-1 | CD31 Exo-Flow Capture Kit |
| EXOFLOW210A-1 | CD44 Exo-Flow Capture Kit |
| EXOFLOW300A-1 | CD63 Exo-Flow Capture Kit |
| EXOFLOW400A-1 | CD81 Exo-Flow Capture Kit |
| EXOFLOW500A-1 | Rab5b Exo-Flow Capture Kit |
| EXOFLOW600A-1 | HLA-G Exo-Flow Capture Kit |
| EXOFLOW610A-1 | CD14 Exo-Flow Capture Kit |
| EXOFLOW620A-1 | CD68 Exo-Flow Capture Kit |
| EXOFLOW630A-1 | EpCAM Exo-Flow Capture Kit |
| EXOFLOW660A-1 | CD73 Exo-Flow Capture Kit |
How It Works
Take a closer look at the Exo-FlowMag96 96-well Magnetic Plate
Once the Exo-Flow32 or Exo-Flow96 clear plastic plate is placed into the Exo-FlowMag96 96-well Magnetic Plate, the magnetic beads stick to the bottom of each well simplifying removal of buffer for washing and elution.
Supporting Data
See isolation data using Exo-FLOW IP Kits
For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.
Figure 1. CD63 and CD9, two exosome markers, are readily detected in samples purified using Exo-Flow Kits. Approximately 1 µg of protein was loaded per well on a 4-20% gradient protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein markers.
Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes.
Citations
- Sheller-Miller, S, et al. (2019) Cre-Reporter Mouse Model to Determine Exosome Communication and Function during Pregnancy. Am. J. Obstet. Gynecol.. 2019 Jun 14;. PM ID: 31207235
- Sheller-Miller, S, et al. (2019) Exosomes Cause Preterm Birth in Mice: Evidence for Paracrine Signaling in Pregnancy. Sci Rep. 2019 Jan 24; 9(1):608. PM ID: 30679631
- Genschmer, KR, et al. (2019) Activated PMN Exosomes: Pathogenic Entities Causing Matrix Destruction and Disease in the Lung. Cell. 2019 Jan 10; 176(1-2):113-126.e15. PM ID: 30633902
- Domenis, R, et al. (2017) Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis. Mediators Inflamm.. 2017 Jun 21; 2017:4814987. PM ID: 28634420
- Meyer, C, et al. (2017) Pseudotyping exosomes for enhanced protein delivery in mammalian cells.. Int J Nanomedicine. 2017 May 1; 12:3153-3170. PM ID: 28458537
- Chen, C, et al. (2017) Mesenchymal stem cell transplantation in tight-skin mice identifies miR-151-5p as a therapeutic target for systemic sclerosis. Cell Res.. 2017 Apr 1; 27(4):559-577. PM ID: 28106077
- Etzerodt, A, et al. (2017) Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of ‘soluble CD163’ in plasma. Sci Rep. 2017 Jan 13; 7:40286. PM ID: 28084321
- Protocol, IIEI. (2017) List of Components. Product. ;. Link: Product
- Smith, JA & Daniel, R. (2016) Human vaginal fluid contains exosomes that have an inhibitory effect on an early step of the HIV-1 life cycle. AIDS. 2016 Nov 13; 30(17):2611-2616. PM ID: 27536982
- Rim, KT & Kim, SJ. (2016) Quantitative Analysis of Exosomes From Murine Lung Cancer Cells by Flow Cytometry. J Cancer Prev. 2016 Sep 1; 21(3):194-200. PM ID: 27722146
- Junker, K, et al. (2016) Extracellular Vesicles and Their Role in Urologic Malignancies.. Eur. Urol.. 2016 Aug 1; 70(2):323-31. PM ID: 26924769
- Mukherjee, K, et al. (2016) Reversible HuR-microRNA binding controls extracellular export of miR-122 and augments stress response. EMBO Rep.. 2016 Aug 1; 17(8):1184-203. PM ID: 27402548
- Wen, Z, et al. (2016) NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs. J. Clin. Invest.. 2016 May 2; 126(5):1953-67. PM ID: 27088800
- Raimondo, F, et al. (2016) Urinary proteomics for the study of genetic kidney diseases.. Expert Rev Proteomics. 2016 Mar 2; 13(3):309-24. PM ID: 26698090
- Nudel, K. (2016) Neisseria gonorrhoeae modulates epithelial cell responses via the induction and release of the inhibitor of apoptosis protein cIAP2 in exosomes. Thesis. ;. Link: Thesis
- Peterson, MF, et al. (2015) Integrated systems for exosome investigation. Methods. 2015 Oct 1; 87:31-45. PM ID: 25916618
- Nudel, K, Massari, P & Genco, CA. (2015) Neisseria gonorrhoeae Modulates Cell Death in Human Endocervical Epithelial Cells through Export of Exosome-Associated cIAP2. Infect. Immun.. 2015 Sep 1; 83(9):3410-7. PM ID: 26077759
- Bhattarai, N, et al. (2015) Conserved Motifs within Hepatitis C Virus Envelope (E2) RNA and Protein Independently Inhibit T Cell Activation. PLoS Pathog.. 2015 Sep 1; 11(9):e1005183. PM ID: 26421924
- Bednarczyk, M, Biryło, M & DAWIDOWICZ, A. (2015) Modern Geodetic Techniques in Spatial Measurement. Thesis. ;. Link: Thesis
- Santana, SM, et al. (2014) Cancerous epithelial cell lines shed extracellular vesicles with a bimodal size distribution that is sensitive to glutamine inhibition. Phys Biol. 2014 Nov 26; 11(6):065001. PM ID: 25426818
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