Home | Products | Exosome Research | Exosome Isolation | The Original ExoQuick

The Original ExoQuick

Fast, scalable exosome isolation from plasma, serum, and ascites fluid.

  • Saves time and labor
  • Is easily scalable
  • Conserves precious sample
  • Delivers high yields of functional, high quality exosomes
  • Can be used to isolate exosomes for a wide range of downstream applications, including biomarker studies, exosomal miRNA profiling, exosomal proteomics, exosomal lipidomics/metabolomics, functional studies, such as in cell-to-cell signaling, and basic biology, such as roles in tumorigenesis.
Catalog Number
Add to Cart
ExoQuick exosome precipitation solution
20 mL
$ 1060
ExoQuick exosome precipitation solution
5 mL
$ 328


A better way to isolate exosomes

“We therefore pursued the ExoQuick® method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models1.”
Need exosomes? SBI’s ExoQuick, original formulation, enables high-throughput, quantitative isolation of exosomes from low volumes (as little as 100 µl) of serum, plasma, or ascites fluid. Compatible with a wide variety of downstream applications, ExoQuick is an effective and proven alternative to ultracentrifugation1-3.

ExoQuick’s fast, ultracentrifugation-free method:

  • Saves time and labor
  • Is easily scalable
  • Conserves precious sample
  • Delivers high yields of functional, high quality exosomes
  • Can be used to isolate exosomes for a wide range of downstream applications, including biomarker studies, exosomal miRNA profiling, exosomal proteomics, exosomal lipidomics/metabolomics, functional studies, such as in cell-to-cell signaling, basic biology, such as role in tumorigenesis.

ExoQuick is a proprietary polymer that gently precipitates exosomes. Add the appropriate amount of ExoQuick to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.

Biofluid Sample volume ExoQuick Volume
Serum, plasma, and ascites fluid. 250 μl 63 μl

In electron microscopy studies, exosomes isolated with ExoQuick appear similar to exosomes isolated using ultracentrifugation1-2, and these exosomes are also active in numerous functional assays1-3.

Exosomes isolated with ExoQuick can be used for all types of protein profiling and protein characterization studies, such as mass spectrometry, Western blotting, ELISA, and more. Higher protein yields are achieved by ExoQuick purification than by chromatography, DynaBeads, or ultracentrifugation.

Exosomes isolated with ExoQuick also provide excellent samples for studying exosome-associated nucleic acids such as microRNAs, siRNAs, and even mRNA. Quantitative analytical techniques such as qPCR, microarray studies, and next-generation sequencing are all compatible with nucleic acids isolated from ExoQuick-purified exosomes.

Backed by a growing number of publications, ExoQuick is often the best option for researchers working with low sample volumes, such as clinical research samples or small animal models.

ExoQuick exosome isolation methods are patented technologies4.

Choose the right ExoQuick for your application:

Application Product Catalog #
Purest EV isolation ExoQuick ULTRA and
General purpose EV isolation ExoQuick and
EV isolation for pre-clinical/in vivo studies ExoQuick-CG EXOCG50A-1
EV isolation that removes contaminating lipoprotein particles from plasma or serum ExoQuick-LP EXOLP5A-1
EV isolation that includes a de-fibrinating plasma step prior to isolation ExoQuick Plasma Prep with Thrombin EXOQ5TM-1


  1. Chugh PE, et al. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies. PLoS Pathog. 2013. 9(7): e1003484. PMCID: PMC3715412.
  1. Umezu T, et al. Leukemia cell to endothelial cell communication via exosomal miRNAs. Oncogene. 2013 May 30. 32(22):2747-55. PMID: 22797057.
  1. Sohel MM, et al. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence. PLoS One. 2013 Nov 4. 8(11):e78505. PMCID: PMC3817212.
  1. Antes T, et al. Methods for Microvesicle Isolation and Selective Removal. Patent No.: US 9,005,888 B2.

How It Works

High-throughput, quantitative exosome recovery

ExoQuick can be used to purify exosomes from plasma1, serum2, and malignant ascites3. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick is an excellent option for researchers who need to purify multiple exosome samples and/or samples from small animal models or clinical research samples.

To isolate exosomes from cleared serum, plasma, or ascites fluid, simply:

  • Add an appropriate volume of ExoQuick to as little as 100 µl sample
  • Incubate for at least one hour at 4°C
  • Isolate exosomes with a 30-minute low-speed spin (1500 g).

Isolated exosomes can be found in the pellet and resuspended in an appropriate solution.

You can verify the presence of exosomes with a number of different methods, including Western blotting for general exosome markers (CD63, CD9, CD81, and HSP70), NanoSight analysis, or EM (learn about different ways to detect exosomes and more in our Exosome Basics Guide).

The Bottom Line
With ExoQuick, you can obtain high-quality exosomes from most biofluids using a protocol that can easily be performed on multiple samples and requires very low volumes of input sample.


  1. Chugh PE, et al. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies. PLoS Pathog. 2013. 9(7): e1003484. PMCID: PMC3715412.
  1. Epple LM, et al. Medulloblastoma Exosome Proteomics Yield Functional Roles for Extracellular Vesicles. PLoS ONE. 2012. 7(7): e42064. PMCID: PMC3407172.
  1. As featured in: Exosome Isolation for Proteomic Analyses and RNA Profiling Douglas D. Taylor, Wolfgang Zacharias and Cicek Gercel-Taylor, Serum/Plasma Proteomics, Methods in Molecular Biology, 2011, Volume 728, Part 4, 235-246.

Supporting Data

Use ExoQuick to isolate exosomes for proteomics and miRNA profiling studies

ExoQuick helps researchers discover protein and RNA biomarkers as well as study exosome biology by enabling fast and quantitative isolation of exosomes.

ExoQuick supports exosomal protein analysis from ascites
Exosomes were isolated from ovarian tumor ascites fluid using ExoQuick, chromatography, DynaBeads, or ultracentrifugation. The ExoQuick method consistently delivered higher concentrations of protein than the other three isolation methods used (Figure 1).


Figure 1. (Top panel) Exosomal proteins were extracted from recovered exosomes, and the amount of protein determined by the Bradford microassay method (Bio-Rad Laboratories), using BSA as a standard. Proteins from each exosome isolate were standardized to the original sample volume and equal volumes were applied per lane of a 12.5% SDS-PAGE gel. (Bottom panel) Western Blotting was performed to analyze the presence of the specific marker protein, placental alkaline phosphatase (PLAP). The bound immune complexes were visualized by enhanced chemiluminescence (ECL, Amersham Life Sciences) and quantitated by densitometry (Un-Scan-it Software, Silk Scientific Corp.).

ExoQuick supports high exosomal miRNA yields
With ExoQuick, you can quickly and easily isolate high quality exosomes for miRNA analysis (Figure 2).

Exosomal microRNAs were recovered from ovarian tumor ascites fluid using either ExoQuick isolation of exosomes followed by Trizol extraction of RNA, Trizol extraction of ovarian tumor ascites fluid with no exosome isolation, or exosome purification using DynaBeads followed by Trizol extraction of RNA. The samples where exosomes were purified using ExoQuick showed the highest yields of microRNAs (Figure 2).

Figure 2. Recovered RNA quality and yield was assessed using a GeneQuant II. Small RNAs were analyzed with the Agilent 2100 Bioanalyzer Lab-on-a-Chip instrument system (Agilent Technologies, Santa Clara, CA), using the Agilent Small RNA chip and reagent kit. Approximately 100 ng of isolated total RNA in 1 µl was applied to each run. The manufacturer’s recommended protocol was strictly followed to obtain Bioanalyzer profiles for the size range 6 to 150 nucleotides (nt). The profiles were calibrated for size (nt) using the small RNA ladder supplied with the kit, containing markers of 20, 40, 60, 80, and 150 nt in size, as reference. The instrument software quantitated the peak area between 0 and 150 nt as small RNA region, the area within 10 to 40 nt as miRNA region, and provides percentages of miRNA detected for each sample.

Characterizing ExoQuick exosomes with NanoSight

Exosomes purified with ExoQuick from serum show the expected particle size distribution and high concentration yields when analyzed using NanoSight’s Nanoparticle Tracking Analysis (NTA, Figure 3).

Figure 3.Exosome size distribution and yields from serum. Exosomes were purified from 50 pooled samples of normal human serum. 250 µl of serum was combined with 63 µl of ExoQuick, incubated at 4°C for thirty minutes, and pelleted by a 1500g spin for thirty minutes. The exosome pellet was resuspended in 100 µl of PBS, diluted 1:10,000, and visualized on the NanoSight LM10 instrument. The analysis shows that the ExoQuick isolation method recovered 90 nm exosomes at a concentration of of 2.74 x 1012 particles/ml.


  • Wang, J, et al. (2018) Determination of Serum Exosomal H19 as a Noninvasive Biomarker for Bladder Cancer Diagnosis and Prognosis. Med. Sci. Monit.. 2018 Dec 21; 24:9307-9316. PM ID: 30576305
  • Zhang, J, et al. (2018) Mouse serum protects against total body irradiation-induced hematopoietic system injury by improving the systemic environment after radiation. Free Radic. Biol. Med.. 2018 Dec 19; 131:382-392. PM ID: 30578918
  • Li, L, et al. (2018) Microenvironmental oxygen pressure orchestrates an anti- and pro-tumoral γδ T cell equilibrium via tumor-derived exosomes. Oncogene. 2018 Dec 13;. PM ID: 30546089
  • Frey, UH, et al. (2018) Remote ischaemic preconditioning increases serum extracellular vesicle concentrations with altered micro-RNA signature in CABG patients. Acta Anaesthesiol Scand. 2018 Dec 11;. PM ID: 30548252
  • Wang, Y, et al. (2018) miR-125a/b inhibits tumor-associated macrophages mediated in cancer stem cells of hepatocellular carcinoma by targeting CD90. J. Cell. Biochem.. 2018 Dec 9;. PM ID: 30536969
  • Zhou, J, et al. (2018) Increased serum exosomal miR-134 expression in the acute ischemic stroke patients. BMC Neurol. 2018 Dec 4; 18(1):198. PM ID: 30514242
  • Abraham, J, Singh, S & Joshi, S. (2018) Liquid biopsy – emergence of a new era in personalized cancer care. Appl Cancer Res. 2018 Dec 1; 38(1). Link: Appl Cancer Res
  • Gao, T, et al. (2018) Exosomal lncRNA 91H is associated with poor development in colorectal cancer by modifying HNRNPK expression. Cancer Cell Int. 2018 Dec 1; 18(1). Link: Cancer Cell Int
  • Yoo, YK, et al. (2018) Toward Exosome-Based Neuronal Diagnostic Devices. Micromachines (Basel). 2018 Nov 29; 9(12). PM ID: 30501125
  • Li, L, et al. (2018) γδTDEs: An Efficient Delivery System for miR-138 with Anti-tumoral and Immunostimulatory Roles on Oral Squamous Cell Carcinoma. Mol Ther Nucleic Acids. 2018 Nov 24; 14:101-113. PM ID: 30594069
  • Barrachina, MN, et al. (2018) Application of Extracellular Vesicles Proteomics to Cardiovascular Disease: Guidelines, Data Analysis, and Future Perspectives. Proteomics. 2018 Nov 23;:e1800247. PM ID: 30467982
  • Matsuura, Y, et al. (2018) Exosomal miR-155 Derived from Hepatocellular Carcinoma Cells Under Hypoxia Promotes Angiogenesis in Endothelial Cells. Dig. Dis. Sci.. 2018 Nov 22;. PM ID: 30465177
  • Zhang, H, et al. (2018) A panel of seven-miRNA signature in plasma as potential biomarker for colorectal cancer diagnosis. Gene. 2018 Nov 17; 687:246-254. PM ID: 30458288
  • Skottvoll, F, et al. (2018) Ultracentrifugation versus kit exosome isolation: nanoLC–MS and other tools reveal similar performance biomarkers, but also contaminations. Future Science OA. 2018 Nov 9;:FSO359. Link: Future Science OA
  • Zhang, W, et al. (2018) Noninvasive prenatal detection of fetal trisomy and single gene disease by shotgun sequencing of placenta originated exosome DNA: a proof-of-concept validation. bioRxiv. 2018 Nov 8;. Link: bioRxiv
  • Xu, H, et al. (2018) Exosomal microRNA-21 derived from bronchial epithelial cells is involved in aberrant epithelium-fibroblast cross-talk in COPD induced by cigarette smoking. Theranostics. 2018 Nov 4; 8(19):5419-5433. Link: Theranostics
  • Nordgren, TM, et al. (2018) Bovine milk-derived extracellular vesicles enhance inflammation and promote M1 polarization following agricultural dust exposure in mice. J. Nutr. Biochem.. 2018 Nov 3; 64:110-120. PM ID: 30476878
  • Raoof, R, et al. (2018) Dual-center, dual-platform microRNA profiling identifies potential plasma biomarkers of adult temporal lobe epilepsy. EBioMedicine. 2018 Nov 2;. PM ID: 30396857
  • Lee, CH, et al. (2018) Discovery of a diagnostic biomarker for colon cancer through proteomic profiling of small extracellular vesicles. BMC Cancer. 2018 Nov 1; 18(1):1058. PM ID: 30382917
  • Brenner, A, Su, G & Momen-Heravi, F. (2018) Isolation of Extracellular Vesicles for Cancer Diagnosis and Functional Studies. Methods in Molecular Biology. 2018 Oct 31;:229-237. Link: Methods in Molecular Biology

Have Questions?

A System Biosciences technical expert is happy to help!

(888) 266-5066 or Contact Us

Sign up to receive technical advice and exclusive deals directly to your inbox.