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HEK293 ACE2 NRP1 Overexpression Stable Cell Line

Advance SARS-CoV-2 research with a ready-to-use stable cell line that overexpresses both ACE2 and NRP1, a facilitator of ACE2-mediated SARS-CoV-2 cell entry

  • Leverage SBI’s robust lentivector expression technology to safely study SARS-CoV-2 cell entry
  • Ready-to-use stable cell line overexpresses both the ACE2 receptor as well as neuropilin-1 (NRP1), a potentiator of ACE2-mediated SARS-CoV-2 cell entry
  • An NRP1 overexpression lentivector is also available as plasmid and pre-packaged virus
  • An ACE2 overexpression lentivector is also available as plasmid and pre-packaged virus
Catalog Number
Description
Size
Price
Quantity
Add to Cart
CVD19-260A-1
HEK293-ACE2-NRP1 Overexpression Stable Cell Line
2 x 10^6 Cells
$ 3499

Overview

Learn more about SARS-CoV-2 with SBI

SBI fuels your SARS-CoV-2 studies with tools that keep up with the latest scientific advances. The HEK293 ACE2 NRP1 Overexpression Stable Cell Line is one example, which we quickly developed in response to publications showing that neuropilin-1 (NRP1) enhances ACE2-mediated cell entry of SARS-CoV-2 [1, 2]. With the HEK293 Ace2 NRP1 Overexpression Stable Cell Line, you can quickly get similar studies running in your own lab, whether to further analyze binding and cell entry, discover inhibitors, or more.

The HEK293 ACE2 NRP1 Overexpression Stable Cell Line takes advantage of our our robust pCDH-CMV-MCS-EF1α-Neo Cloning and Expression Lentivector (Cat.# CD514B-1), delivering NRP1 expression from the CMV promoter with a separate EF1α promoter driving the expression of the neomycin resistance gene for selection.

  • Leverage SBI’s robust lentivector expression technology to safely study SARS-CoV-2 cell entry
  • Ready-to-use stable cell line overexpresses both the ACE2 receptor as well as neuropilin-1 (NRP1), a potentiator of ACE2-mediated SARS-CoV-2 cell entry
  • An NRP1 overexpression lentivector is also available as plasmid and pre-packaged virus
  • An ACE2 overexpression lentivector is also available as plasmid and pre-packaged virus

 

See all of our COVID-19-related Expression Products

Catalog NumberProduct name
CVD19-100PA/VA-1ACE2 Expression Lentivector, pCDH-CMV-ACE2-EF1α-Puro (Plasmid or Pre-packaged Lentivirus)
CVD19-140PA/VA-1NRP1 Expression Lentivector, pCDH-CMV-NRP1-EF1α-Neo (Plasmid or Pre-packaged Lentivirus)
CVD19-120PA/VA-1SARS-CoV-2 Nucleocapsid (N protein) Expression Lentivector, pCDH-CMV-SARS-CoV-2-N-EF1α-Puro (Plasmid or Pre-packaged Lentivirus)
CVD19-130PA/VA-1SARS-CoV-2 Spike Glycoprotein (S) Expression Lentivector, pCDH-CMV-SARS-CoV-2-S-EF1α-Puro (Plasmid or Pre-packaged Lentivirus)
CVD19-135PA/VA-1SARS-CoV-2 Humanized Spike Glycoprotein (S) Expression Lentivector, pCDH-CMV-SARS-CoV-2-hS-EF1α-Puro (Plasmid or Pre-packaged Lentivirus)
CVD19-200A-1HEK293 ACE2 Stable Cell Line
CVD19-260A-1HEK293-ACE2-NRP1 Overexpression Stable Cell Line
CVD19-220A-1HEK293 "N" SARS-CoV-2 Nucleocapsid Overexpression Stable Cell Line
CVD19-420A-1
CVD19-425A-1
"N" SARS-CoV-2 Nucleocapsid mRNA
CVD19-430A-1
CVD19-435A-1
"S" SARS-CoV-2 Spike Glycoprotein mRNA

References

  1. Cantuti-Castelvetri L, et al. Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity. Science. 2020 Nov 13;370(6518):856-860. PMCID: PMC7857391.
  2. Daly JL, et al. Neuropilin-1 is a host factor for SARS-CoV-2 infection. Science. 2020 Nov 13;370(6518):861-865. PMID: 33082294.

Supporting Data

 

Figure 1. Both ACE2 and NRP1 are well expressed in the HEK293 ACE2 NRP1 Overexpression Stable Cell Line.  To demonstrate the robust expression of ACE2 and NRP1 in the HEK293 ACE2 NRP1 Overexpression Stable Cell Line, we extracted total protein and analyzed ACE2 (A) and NRP1 (B) expression via western blot. The parental HEK293 cells display no ACE2 (A) or NRP1 (B) expression whereas the HEK293 ACE2 NRP1 Stable Cell Line possesses robust ACE2 expression (A) and NRP1 expression (B). The positive control in B is HEK293 cells transfected with the the NRP1 Expression Lentivector.

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