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CMV-Luciferase-RFP-TK Lentivector for In Vivo Imaging

Use this vector to track cell fate after implantation, or for other applications requiring in vivo imaging – delivers CMV-driven RFP, luciferase, and TK

  • You’re working with difficult-to-transfect cells
  • You’d like to create stable reporter cell lines
  • You’d like to integrate your reporter into the genome
  • You’d like episomal expression
  • You’d like to avoid introducing foreign sequences
Catalog Number
Description
Size
Price
Quantity
Add to Cart
BLIV102PA-1
Lentiviral Triple Reporter: CMV-Luciferase-RFP-TK plasmid
10 µg
$ 693
BLIV102VA-1
Lentiviral Triple Reporter: CMV-Luciferase-RFP-TK pre-packaged virus
>2 x10^6 IFUs
$ 693

Overview

Clearly visualize cells in vivo and ex vivo for tracking cell fate and more

Easily label cell lines for tracking cell fate after implantation in an animal model or other imaging applications with SBI’s line of Bioluminescent, Fluorescent, and PET Imaging Vectors (BLIV). Available in a variety of promoter-reporter combinations that enable multiple imaging modalities—fluorescence with copGFP, bioluminescence with luciferase, and positron emission tomography (PET) with thymidine kinase (TK)—you can select from lentivector or minicircle technologies for both integrated and episomal expression.

SBI’s lentivector-based imaging systems offer safe, stable, and effective gene delivery in virtually all mammalian cells in tissue culture and in whole animal models.

The CMV-Luciferase-RFP-TK Lentivector for In Vivo Imaging leverages SBI’s well-regarded lentivector technology for either transduction or transfection of the reporter into target cells. Because the vector becomes integrated into the genome, you can use this vector to create stable reporter cell lines.

The vector expresses RFP, luciferase, and TK from a CMV promoter for strong expression in most common cell types, such as HeLa, HEK293, or HT1080. Available as either a plasmid or pre-packaged virus.

Which BLIV construct should you choose?

With a range of options, SBI’s vectors for in vivo imaging support a wide range of projects. Simply choose the vector that best fits your needs:

Choose a lentivector when:

  • You’re working with difficult-to-transfect cells
  • You’d like to create stable reporter cell lines
  • You’d like to integrate your reporter into the genome

Choose a minicircle vector* when you’re working with transfectable cells and:

  • You’d like episomal expression
  • You’d like to avoid introducing foreign sequences

Choose your promoter using the following table:

Promoter Expression Level Application
CMV High Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCV High Hematopoietic and stem cells
EF1α Medium Most cell types including primary cells and stem cells
PGK Medium Most cell types including primary cells and stem cells
UbC Low Most cell types including primary cells and stem cells

*Note that Minicircle Parental Vectors are only available to academic/non-profit customers. Researchers at commercial organization may only purchase the pre-made minicircle BLIVs.

Supporting Data

See SBI’s BLIV Vectors in action

Figure 1. Examples of how SBI’s BLIV lentivectors deliver strong luciferase activity after both transduction and transfection.

Figure 2. Examples of how SBI’s BLIV minicircle vectors deliver strong luciferase activity in small animal models.

Figure 3. Examples of how SBI’s BLIV lentivectors and minicircle vectors can be used to generate stable cell lines with strong luciferase activity.

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