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miRZip™ Pooled Anti-miRNA Virus Library

Efficiently conduct high-throughput phenotypic screens by quickly building stable cell lines where a specific miR is knocked down.

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MZIPPLVA-1
miRZip™ Pooled Anti-miRNA Virus Library
1 Virus Aliquot
$ 1010

Overview

Simplifying high-throughput phenotypic screening for miR function

Take your miR studies to the next level with SBI’s pooled miRZip™ anti-miRNA Lentivirus Library. Quickly and efficiently create stable miR knockdowns in the cell line of your choice for high-throughput phenotypic screening. SBI’s miRZip constructs leverage our powerful and well-regarded third-generation lentivector technology to deliver short hairpin RNAs (shRNA) that, after processing, preferentially produce an anti-sense miRNA. The hairpin is rationally designed to be asymmetric, ensuring that the sense strand does not contain the endogenous miRNA sequence and enabling accumulation of the anti-miRNA. The result is robust derepression of the transcripts targeted by the microRNA being “zipped,” and elevation of the corresponding protein levels.

Study miRs with SBI’s powerful miRZip technology:

  • Stable and permanent anti-microRNA expression from a constitutive H1 promoter
  • Rationally designed, asymmetric hairpins optimized for anti-sense microRNA production
  • Efficient suppression of specific endogenous microRNAs
  • Reliable delivery to dividing or non-dividing cells
  • Selection/sorting for transfected/transduced cells with either copGFP or puromycin

Conduct high-throughput phenotypic screens with our pool of pre-packaged lentiviral particles containing our entire miRZip collection (Cat.# MZIPPLVA-1).

How It Works

Screening with the miRZip Library is straightforward

  • Transduce the miRZip Library into target cells
  • Treat to induce phenotype
  • Use a functional assay (invasion assay, differentiation assay, proliferation assay, apoptosis assay, etc.) to select for cells exhibiting the desired phenotype
  • Identify miR effectors by amplifying effector miR and sequencing

Supporting Data

See SBI’s mirZip technology in action

miRZip constructs express high levels of anti-microRNAs

Figure 1. miRZip constructs express high levels of anti-microRNAs. miRZip constructs were transfected into HEK293 cells, total RNA isolated, and miRZip levels measured using SBI’s QuantiMir Assay (Cat.# RA420A-1). Compared to QuantiMir assays performed on RNA isolated from non-transfected cells, the miRZip-transfected cells delivered high expression levels of the specific miRZip anti-miRNAs.

Efficient modulation of target protein levels by SBI’s Lenti-miR and miRZip technologies

Figure 2. Target protein levels are efficiently modulated by SBI’s Lenti-miR-29a and miRZip-29a microRNA constructs.

miRZip technology is effective in a functional assay

Figure 3. miRZip-21 and miRZip-145 exhibit anti-oncogenic and oncogenic activity, respectively, in a cell invasion assay using MDA-MB-231 breast cancer cells. (Top panel) miR-21 is an oncogenic miRNA and miR-145 is a tumor suppressing miRNA. Transduction of miRZip-21 (the anti-miR21 lentiviral construct) and miRZip-145 (the anti-miR145 lentiviral construct), inhibited the respective miRs, resulting in a lower percentage of invading cells in miRZip-21-transduced cells and a higher percentage of invading cells in miRZip-145-transduced cells. (Bottom panel) Additionally, the miRZip-145 lentivirus inhibited endogenous miR-145 and elevated protein expression levels of the miR-145 target oncogene c-Myc.

Citations

  • Liu, A, et al. (2017) Antagonizing miR-455-3p inhibits chemoresistance and aggressiveness in esophageal squamous cell carcinoma. Mol. Cancer. 2017 Jun 21; 16(1):106. PM ID: 28633632
  • Wu, Q, et al. (2017) Downregulation of microRNA-448 improves isoflurane-induced learning and memory impairment in rats. Molecular Medicine Reports. 2017 Jun 8;:1578-1583. Link: Molecular Medicine Reports
  • Sun, J, et al. (2017) Mir-367 is downregulated in coronary artery disease and its overexpression exerts anti-inflammatory effect via inhibition of the NF-κB-activated inflammatory pathway. International Journal of Clinical and Experimental Pathology. 2017 Apr 15; 10(4):4047-4057. Link: International Journal of Clinical and Experimental Pathology
  • Guo, GC, et al. (2017) microRNA-761 induces aggressive phenotypes in triple-negative breast cancer cells by repressing TRIM29 expression. Cell Oncol (Dordr). 2017 Apr 1; 40(2):157-166. PM ID: 28054302
  • Yang, TB, et al. (2017) Mutual reinforcement between telomere capping and canonical Wnt signalling in the intestinal stem cell niche. Nat Commun. 2017 Mar 17; 8:14766. PM ID: 28303901
  • Su, K, et al. (2017) miR-940 upregulation contributes to human cervical cancer progression through p27 and PTEN inhibition.. Int. J. Oncol.. 2017 Mar 7;. PM ID: 28350106
  • Moyal, L, et al. (2017) Oncogenic role of microRNA-155 in mycosis fungoides: an in vitro and xenograft mouse model study. Br. J. Dermatol.. 2017 Mar 3;. PM ID: 28256712
  • Li, G, et al. (2017) The microRNA-182-PDK4 axis regulates lung tumorigenesis by modulating pyruvate dehydrogenase and lipogenesis. Oncogene. 2017 Feb 16; 36(7):989-998. PM ID: 27641336
  • Teteloshvili, N, et al. (2017) Argonaute 2 immunoprecipitation revealed large tumor suppressor kinase 1 as a novel proapoptotic target of miR-21 in T cells. FEBS J.. 2017 Feb 1; 284(4):555-567. PM ID: 28075055
  • Spitschak, A, et al. (2017) MiR-182 promotes cancer invasion by linking RET oncogene activated NF-κB to loss of the HES1/Notch1 regulatory circuit. Mol. Cancer. 2017 Jan 26; 16(1):24. PM ID: 28122586
  • Beezhold, K, Klei, LR & Barchowsky, A. (2017) Regulation of cyclin D1 by arsenic and microRNA inhibits adipogenesis. Toxicol. Lett.. 2017 Jan 4; 265:147-155. PM ID: 27932253
  • Poon, VY, et al. (2016) miR-27b shapes the presynaptic transcriptome and influences neurotransmission by silencing the polycomb group protein Bmi1. BMC Genomics. 2016 Oct 4; 17(1):777. PM ID: 27716060
  • Shen, X, et al. (2016) miR-322/-503 cluster is expressed in the earliest cardiac progenitor cells and drives cardiomyocyte specification. Proc. Natl. Acad. Sci. U.S.A.. 2016 Aug 23; 113(34):9551-6. PM ID: 27512039
  • Langsch, S, et al. (2016) miR-29b Mediates NF-κB Signaling in KRAS-Induced Non-Small Cell Lung Cancers.. Cancer Res.. 2016 Jul 15; 76(14):4160-9. PM ID: 27199349
  • Wang, S, et al. (2016) MicroRNA-mediated epigenetic targeting of Survivin significantly enhances the antitumor activity of paclitaxel against non-small cell lung cancer. Oncotarget. 2016 Jun 21; 7(25):37693-37713. PM ID: 27177222
  • Zheng, D, et al. (2016) Inhibition of MicroRNA 195 Prevents Apoptosis and Multiple-Organ Injury in Mouse Models of Sepsis. J. Infect. Dis.. 2016 May 15; 213(10):1661-70. PM ID: 26704614
  • Tan, Z, et al. (2016) MicroRNA-1229 overexpression promotes cell proliferation and tumorigenicity and activates Wnt/β-catenin signaling in breast cancer. Oncotarget. 2016 Apr 26; 7(17):24076-87. PM ID: 26992223
  • Lin, SC, et al. (2016) Dysregulation of miRNAs-COUP-TFII-FOXM1-CENPF axis contributes to the metastasis of prostate cancer. Nat Commun. 2016 Apr 25; 7:11418. PM ID: 27108958
  • Hong, X, et al. (2016) MiR-448 promotes glycolytic metabolism of gastric cancer by downregulating KDM2B. Oncotarget. 2016 Apr 19; 7(16):22092-102. PM ID: 26989077
  • Liang, ML, et al. (2016) Downregulation of miR-137 and miR-6500-3p promotes cell proliferation in pediatric high-grade gliomas. Oncotarget. 2016 Apr 12; 7(15):19723-37. PM ID: 26933822

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