Home | Products | Gene Expression Systems | Minicircle Technology | Mouse IL-23 Pre-made Minicircle DNA (RSV->FLAG-mP40-mP19-pA)

Mouse IL-23 Pre-made Minicircle DNA (RSV->FLAG-mP40-mP19-pA)

Study mouse models of inflammation with this ready-to-transfect Mouse IL-23 Pre-made RSV->FLAG-mP40-mP19-pA Minicircle DNA

  • Episomal expression sustained over weeks
  • Foreign DNA-free
  • More efficient transfections from small plasmid size
  • Unlimited insert size
  • Optimized coli minicircle production strain
Catalog Number
Add to Cart
Mouse IL-23 pre-made Minicircle DNA (RSV->FLAG-mP40-mP19-pA)
100 µg
$ 814


Study inflammation in a mouse model

With the ready-to-transfect/inject Mouse IL-23 Pre-made RSV->FLAG-mP40-mP19-pA Minicircle DNA (Cat.# MN651MC-1), you can get your mouse inflammation studies off the ground fast. Get sustained, low level expression of the IL-23 protein in serum for studies where you want controlled levels of IL-23.

About Minicircle Technology

  • Episomal expression sustained over weeks
  • Foreign DNA-free
  • More efficient transfections from small plasmid size
  • Unlimited insert size
  • Optimized coli minicircle production strain
  • Works in vitro and in vivo

When you want sustained transgene expression without introducing any foreign DNA—such as for model animal and gene therapy development—Minicircle Technology is a great gene expression option. Produced as small excised, circular DNA fragments from a parental plasmid, the non-viral, episomal Minicircle expression cassette is free of any bacterial plasmid DNA sequences, and comes with a variety of promoter and reporter combinations. Their small size facilitates more efficient transfection than what’s possible with standard-sized plasmids, and, while Minicircles do not replicate with the host cell, expression lasts for 14 days or longer in dividing cells, and can continue for months in non-dividing cells.

Product Note:

Parental minicircle plasmids and the ZYCY10P3S2T Producer Bacterial Strain are available for purchase by not-for-profit researchers only. Commercial users may purchase pre-made, ready-to-transfect minicircle DNA only. SBI also offers custom parental plasmid cloning and minicircle DNA production to both not-for-profit and commercial end users—contact services@systembio.com for additional details. For any other purposes, including the ability to buy the parental MC production system, commercial users should contact SBI at tech@systembio.com for further information.

How It Works

Generating minicircles from the parental cloning vector

To generate minicircles that are ready for transfection, you need your Minicircle Cloning Vector with your insert (gene, promoter-gene cassette, small RNA, etc.), SBI’s optimized, ready-to-transform ZYCY10P3S2T E. coli Minicircle Producer Strain (Cat.# MN900A-1), and arabinose (Cat.# MN850A-1).

Minicircles are produced from the full-sized Parental Minicircle using PhiC31 Integrase, which mediates a recombination event between the PhiC321 attB and attP sites on the parental plasmid (Figure 1). This reaction results in two products—the minicircle, which is now free from any bacterial DNA sequences—and the parental plasmid. To get rid of the parental plasmid, the I-SceI endonuclease recognizes and acts on the I-SceI sites on the parental plasmid, resulting in degradation of the parental plasmid.

Figure 1. Generating minicircle DNA from the Parental Minicircle Plasmid.

More about the ZYCY10P3S2T E. coli Minicircle Producer Strain

The Minicircle Producer Strain harbors an arabinose-inducible system to express the PhiC31 integrase and the I-SceI endonuclease simultaneously. The ZYCY10P3S2T strain also contains a robust arabinose transporter LacY A177C gene. Adding arabinose to the media turns on expression of the PhiC31 integrase and endonuclease genes, resulting in separation of the Parental Minicircle Plasmid into the individual minicircle and parental plasmids (from the PhiC31 Integrase activity), and the degradation of the parental plasmid (from Sce-1 endonuclease activity).

Supporting Data

Achieve sustained expression from minicircles after transfection in vitro and in vivo

Figure 1. Easy, sustained transfection in most cell types. Transfection of 1 μg of minicircle DNA (pMC.CMV-MCS-EF1-GFPSV40PolyA, Cat.# MN511A-1) into HEK293 cells delivers over one week of robust gene expression.

Figure 2. Express transgenes for weeks in animal models. (A) Hydrodynamic tail vein injection of 2 µg and 4 µg of minicircle DNA (CMV-GFP-Luc) into mice shows excellent expression after 48 hours. (B) Minicircle-delivered transgenes retain robust expression that can last for weeks compared to transgenes that are delivered using plasmid DNA, where expression is rapidly lost. In this study, 40 µg of minicircle DNA was introduced into mice via hydrodynamic tail vein injection.


  • Johnston, CD, et al. (2019) Systematic evasion of the restriction-modification barrier in bacteria. Proc. Natl. Acad. Sci. U.S.A.. 2019 May 16;. PM ID: 31097593
  • Han, D, et al. (2019) Activation of Melatonin Receptor 2 But Not Melatonin Receptor 1 Mediates Melatonin-conferred Cardio-protection Against Myocardial Ischemia/Reperfusion Injury. J. Pineal Res.. 2019 Mar 22;:e12571. PM ID: 30903623
  • Traub, S, et al. (2017) Pharmaceutical Characterization of Tropomyosin Receptor Kinase B-Agonistic Antibodies on Human Induced Pluripotent Stem (hiPS) Cell-Derived Neurons. J. Pharmacol. Exp. Ther.. 2017 Jun 1; 361(3):355-365. PM ID: 28351853
  • Petrini, S, et al. (2017) Aged induced pluripotent stem cell (iPSCs) as a new cellular model for studying premature aging. Aging (Albany NY). 2017 May 31; 9(5):1453-1469. PM ID: 28562315
  • Henno, L, et al. (2017) Analysis of Human Papillomavirus Genome Replication Using Two- and Three-Dimensional Agarose Gel Electrophoresis. Curr Protoc Microbiol. 2017 May 16; 45:14B.10.1-14B.10.37. PM ID: 28510360
  • Zhang, Z, et al. (2017) Gene delivery of TIPE2 inhibits breast cancer development and metastasis via CD8(+) T and NK cell-mediated antitumor responses.. Mol. Immunol.. 2017 May 1; 85:230-237. PM ID: 28314212
  • Tidd, N, et al. (2017) Minicircle Mediated Gene Delivery to Canine and Equine Mesenchymal Stem Cells. Int J Mol Sci. 2017 Apr 12; 18(4). PM ID: 28417917
  • Kelton, W, et al. (2017) Reprogramming MHC specificity by CRISPR-Cas9-assisted cassette exchange. Sci Rep. 2017 Apr 4; 7:45775. PM ID: 28374766
  • Wu, H, et al. (2017) MicroRNA-206 prevents hepatosteatosis and hyperglycemia by facilitating insulin signaling and impairing lipogenesis. J. Hepatol.. 2017 Apr 1; 66(4):816-824. PM ID: 28025059
  • Liu, N, et al. (2017) PIM1-minicircle as a therapeutic treatment for myocardial infarction. PLoS ONE. 2017 Mar 21; 12(3):e0173963. PM ID: 28323876
  • Jaafar, L, et al. (2017) SFPQ•NONO and XLF function separately and together to promote DNA double-strand break repair via canonical nonhomologous end joining. Nucleic Acids Res.. 2017 Feb 28; 45(4):1848-1859. PM ID: 27924002
  • Brett, E, et al. (2017) Magnetic Nanoparticle-Based Upregulation of B-Cell Lymphoma 2 Enhances Bone Regeneration. Stem Cells Transl Med. 2017 Jan 1; 6(1):151-160. PM ID: 28170185
  • Colluru, VT, Zahm, CD & McNeel, DG. (2016) Mini-intronic plasmid vaccination elicits tolerant LAG3(+) CD8(+) T cells and inferior antitumor responses. Oncoimmunology. 2016 Nov 17; 5(10):e1223002. PM ID: 27853647
  • Li, F, et al. (2016) Minicircle HBV cccDNA with a Gaussia luciferase reporter for investigating HBV cccDNA biology and developing cccDNA-targeting drugs. Sci Rep. 2016 Nov 7; 6:36483. PM ID: 27819342
  • Tockner, B, et al. (2016) Construction and validation of an RNA trans-splicing molecule suitable to repair a large number of COL7A1 mutations. Gene Ther.. 2016 Nov 1; 23(11):775-784. PM ID: 27434145
  • Gaspar, VM, et al. (2016) Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate. Separation and Purification Technology. 2016 Oct 31; 174:417–424. Link: Separation and Purification Technology
  • Fernandes, AR & Chari, DM. (2016) Part II: Functional delivery of a neurotherapeutic gene to neural stem cells using minicircle DNA and nanoparticles: Translational advantages for regenerative neurology. J Control Release. 2016 Sep 28; 238:300-10. PM ID: 27369863
  • Fernandes, AR & Chari, DM. (2016) Part I: Minicircle vector technology limits DNA size restrictions on ex vivo gene delivery using nanoparticle vectors: Overcoming a translational barrier in neural stem cell therapy. J Control Release. 2016 Sep 28; 238:289-99. PM ID: 27317366
  • Mun, JY, et al. (2016) Minicircle microporation-based non-viral gene delivery improved the targeting of mesenchymal stem cells to an injury site.. Biomaterials. 2016 Sep 1; 101:310-20. PM ID: 27315214
  • Diamantino, T, et al. (2016) Minicircle DNA purification using a CIM® DEAE-1 monolithic support. J Sep Sci. 2016 Sep 1; 39(18):3544-9. PM ID: 27600622

Have Questions?

A System Biosciences technical expert is happy to help!

(888) 266-5066 or Contact Us

Sign up to receive technical advice and exclusive deals directly to your inbox.