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Mouse RORγt Promoter GreenFire Lentivector

Study the balance between tolerance and inflammation with this vector that uses the mouse RORγt promoter to drive both GFP and luciferase expression

Catalog Number
Description
Size
Price
Quantity
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TCL800A-1
Mouse RORyt Promoter GreenFire lentivector plasmid
10 µg
$ 618

Overview

Monitor the balance of T cell dynamics in autoimmunity, inflammation, and cancer

Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines. With the Mouse RORγt Promoter GreenFire Lentivector you can combine SBI’s well-regarded and high quality lentivector technology with a RORγt promoter-reporter architecture that enables easy and quantitative monitoring of RORγt expression. The RORγt promoter drives coordinated expression of GFP and luciferase reporters.

The promoter sequences and design are based upon Dang, et al.1, Lazarevic, et al.2, and Ruan, et al3.

Supporting Data

Luciferase activation in response to RORγt transcriptional activators

Figure 1. SBI’s GreenFire Lentivector reporters for mFoxp3, mRORγt, and mIL-17 show strong luciferase activity in response to known transcriptional activators. METHODS: Human Jurkat T cells were transduced with lentivirus for the Foxp3, RORγt and IL-17 promoter reporters. Transcription activation was tested using stimulation through the addition of with PMA (5 ng/ml) and Ionomycin (500 ng/ml). Cells were harvested after 24 hours and luciferase activity measured for transcription activation responses.

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