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“N” SARS-CoV-2 Nucleocapsid mRNA

Transiently express the SARS-CoV-2 nucleocapsid protein (N protein) in the cells of your choice with this ready-to-transfect mRNA.

  • Speed your studies with ready-to-transfect mRNA
  • Transiently express the native (not codon optimized) SARS-CoV-2 nucleocapsid protein
  • Stable SARS-CoV-2 nucleocapsid protein expression is also available as a stable cell line, pre-packaged transduction-ready lentivirus, and ready-to-package lentivector
Catalog Number
Description
Size
Price
Quantity
Add to Cart
CVD19-420A-1
“N” SARS-CoV-2 Nucleocapsid mRNA (10 µg, Transfection-ready)
10 µg
$ 315
CVD19-425A-1
“N” SARS-CoV-2 Nucleocapsid mRNA (20 µg, Transfection-ready)
20 µg
$ 449

Overview

Advance your COVID-19 studies with SBI

With transfection-ready “N” SARS-CoV-2 Nucleocapsid mRNA, you can go right to studying the native SARS-CoV-2 nucleocapsid protein from the original Wuhan-1 strain. The “N” SARS-CoV-2 Nucleocapsid mRNA delivers robust transient expression to support a range of nucleocapsid protein studies.

 

See all of our COVID-19-related Expression Options

Catalog NumberConstruct
CVD19-100PA/VA-1ACE2 Expression Lentivector, pCDH-CMV-ACE2-EF1α-Puro (Plasmid or Pre-packaged Lentivirus)
CVD19-120PA/VA-1SARS-CoV-2 Nucleocapsid (N protein) Expression Lentivector, pCDH-CMV-SARS-CoV-2-N-EF1α-Puro (Plasmid or Pre-packaged Lentivirus)
CVD19-130PA/VA-1SARS-CoV-2 Spike Glycoprotein (S) Expression Lentivector, pCDH-CMV-SARS-CoV-2-S-EF1α-Puro (Plasmid or Pre-packaged Lentivirus)
CVD19-135PA/VA-1SARS-CoV-2 Humanized Spike Glycoprotein (S) Expression Lentivector, pCDH-CMV-SARS-CoV-2-hS-EF1α-Puro (Plasmid or Pre-packaged Lentivirus)
CVD19-200A-1HEK293 ACE2 Stable Cell Line
CVD19-220A-1HEK293 "N" SARS-CoV-2 Nucleocapsid Overexpression Stable Cell Line
CVD19-420A-1
CVD19-425A-1
"N" SARS-CoV-2 Nucleocapsid mRNA
CVD19-430A-1
CVD19-435A-1
"S" SARS-CoV-2 Spike Glycoprotein mRNA

 

Supporting Data

“N” SARS-CoV-2 Nucleocapsid mRNA is of high-quality

The high quality of the “N” SARS-CoV-2 Nucleocapsid mRNA can be seen in Figure 1, which shows that the in vitro transcribed mRNA migrates as a single species on a 1.5% denaturing agarose gel.

Figure 1. The “N” SARS-CoV-2 mRNA is a high-quality mRNA that migrates as a single species.

“N” SARS-CoV-2 Nucleocapsid mRNA is well expressed in HEK293T cells

To demonstrate the robust expression of the “N” SARS-CoV-2 Nucleocapsid mRNA, we transfected 1 µg into HEK293T cells and isolated total protein after two days. We analyzed 15 µg of total protein via western blot, probing for both the nucleocapsid protein and β-actin. The high expression of nucleocapsid protein can be seen in Figure 2.

Figure 2. The “N” SARS-CoV-2 Nucleocapsid mRNA is well expressed in HEK293T cells.

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