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PB-Cuo-shMCS-IRES-GFP-EF1α-CymR-Puro Inducible shRNA Cloning and Expression Vector

Combining easy PiggyBac transgenesis with our robust and titratable cumate-inducible expression system, this PiggyBac Vector is designed for shRNA expression

  • Make transgenic cell lines with a single transfection
  • Integrate multiple PiggyBac Vectors in a single transfection
  • Insert an expression cassette into human, mouse, and rat cells
  • Deliver virtually any-sized DNA insert, from 10 – 100 kb
  • Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
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PBQMSH812A-1
PB-Cuo-shMCS-IRES-GFP-EF1α-CymR-Puro Inducible shRNA Cloning and Expression Vector
10 µg
$ 962

Overview

Robust, titratable shRNA expression delivered using the PiggyBac Transposon System

More than just easy, consistent transgenesis with PiggyBac, the PB-Cuo-shMCS-IRES-GFP-EF1α-CymR-Puro Inducible shRNA Cloning and Expression Vector (Cat.# PBQMSH812A-1) adds in the robust and titratable gene expression control of SBI’s cumate-inducible expression system. Clone your shRNA-of-interest into the shMCS for cumate-inducible expression, which you can quantitatively monitor with the co-expressed GFP. This vector also co-expresses the cumate repressor, CymR, and puromycin resistance from the EF1α promoter.

With the PiggyBac Transposon System, you can:

  • Make transgenic cell lines with a single transfection
  • Integrate multiple PiggyBac Vectors in a single transfection
  • Insert an expression cassette into human, mouse, and rat cells
  • Deliver virtually any-sized DNA insert, from 10 – 100 kb
  • Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
  • Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)

Customer Agreements
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a four-month, limited-use license to evaluate the technology.
For end user license information, see the following:

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.

How It Works

The PiggyBac Transposon System’s Cut-and-Paste Mechanism

The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.

Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.

  • The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
  • The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
  • The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal

Tightly-controlled, inducible gene expression

Get robust, titratable gene expression with low background using SBI’s cumate-inducible vectors. These vectors take advantage of CymR, a repressor that binds to cumate operator sequences (CuO) with high affinity in the absence of cumate, a non-toxic small molecule. Providing much lower background expression than similar systems, SBI’s cumate-inducible vectors can provide up to 32-fold induction of gene expression.

  • Robust—increase expression up to 32-fold
  • Adjustable—tune expression levels by titrating the amount of cumate
  • Reversible—turn expression on, then off, then on again
  • Powerful—suitable for in vivo applications

Supporting Data

Tight expression control with low background

Figure 2. In the absence of cumate, the cumate-inducible PiggyBac Vector shows undetectable levels of expression.

Figure 3. The PiggyBac cumate switch is titratable and can be turned off.

Citations

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  • Denes, LT, et al. (2019) Culturing C2C12 myotubes on micromolded gelatin hydrogels accelerates myotube maturation. Skelet Muscle. 2019 Jun 7; 9(1):17. PM ID: 31174599
  • Richter, JF, et al. (2019) Occludin knockdown is not sufficient to induce transepithelial macromolecule passage. Tissue Barriers. 2019 Jun 4; 7(2):1612661. PM ID: 31161924
  • Mao, X, et al. (2019) Schedule-dependent potentiation of chemotherapy drugs by the hypoxia-activated prodrug SN30000. Cancer Biol. Ther.. 2019 May 26;:1-12. PM ID: 31131698
  • Chapnick, DA, et al. (2019) Temporal Metabolite, Ion, and Enzyme Activity Profiling Using Fluorescence Microscopy and Genetically Encoded Biosensors. Methods Mol. Biol.. 2019 May 24; 1978:343-353. PM ID: 31119673
  • Shinmura, K, et al. (2019) POLQ Overexpression Is Associated with an Increased Somatic Mutation Load and PLK4 Overexpression in Lung Adenocarcinoma. Cancers (Basel). 2019 May 24; 11(5). PM ID: 31137743
  • Li, F, et al. (2019) A piggyBac-based TANGO GFP assay for high throughput screening of GPCR ligands in live cells. Cell Commun. Signal. 2019 May 23; 17(1):49. PM ID: 31122241
  • Shrestha, M, et al. (2019) The transition of tissue inhibitor of metalloproteinases from -4 to -1 induces aggressive behavior and poor patient survival in dedifferentiated liposarcoma via YAP/TAZ activation. Carcinogenesis. 2019 May 10;. PM ID: 31074490
  • Gan, L, et al. (2019) The lysosomal GPCR-like protein GPR137B regulates Rag and mTORC1 localization and activity. Nat. Cell Biol.. 2019 May 1; 21(5):614-626. PM ID: 31036939
  • Ahmad, ST, et al. (2019) Capicua regulates neural stem cell proliferation and lineage specification through control of Ets factors. Nat Commun. 2019 May 1; 10(1):2000. PM ID: 31043608
  • Rivera, FJ, et al. (2019) Aging restricts the ability of mesenchymal stem cells to promote the generation of oligodendrocytes during remyelination. Glia. 2019 Apr 30;. PM ID: 31038798
  • Inoue, M, et al. (2019) Structural Basis of Sarco/Endoplasmic Reticulum Ca2+-ATPase 2b Regulation via Transmembrane Helix Interplay. Cell Rep. 2019 Apr 23; 27(4):1221-1230.e3. PM ID: 31018135
  • Bielczyk-Maczyńska, E, et al. (2019) Loss of adipocyte identity through synergistic repression of PPARγ by TGF-β and mechanical stress. bioRxiv. 2019 Apr 11;. Link: bioRxiv
  • Sakahara, M, et al. (2019) IFN/STAT signaling controls tumorigenesis and the drug response in colorectal cancer. Cancer Sci.. 2019 Apr 1; 110(4):1293-1305. PM ID: 30724425
  • Paydarnia, N, et al. (2019) Synergistic effect of granzyme B-azurin fusion protein on breast cancer cells. Mol. Biol. Rep.. 2019 Apr 1;. PM ID: 30937652
  • Jones, EM, et al. (2019) A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing. Cell Syst. 2019 Mar 27; 8(3):254-260.e6. PM ID: 30904378
  • Sauter, EJ, et al. (2019) Induced Neurons for the Study of Neurodegenerative and Neurodevelopmental Disorders. Methods Mol. Biol.. 2019 Mar 23; 1942:101-121. PM ID: 30900179
  • Laugsch, M, et al. (2019) Modeling the Pathological Long-Range Regulatory Effects of Human Structural Variation with Patient-Specific hiPSCs. Cell Stem Cell. 2019 Mar 21;. PM ID: 30982769
  • Farhadi, A, et al. (2019) Ultrasound Imaging of Gene Expression in Mammalian Cells. bioRxiv. 2019 Mar 18;. Link: bioRxiv
  • Garivet, G, et al. (2019) Small-Molecule Inhibition of the UNC-Src Interaction Impairs Dynamic Src Localization in Cells. Cell Chem Biol. 2019 Mar 13;. PM ID: 30956149

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