Cytosol Cyto-Tracer™, pCT-Cyto-RFP (CMV)

Overview

Easily study the dynamics of cellular processes with SBI’s Cyto-Tracers™

Leverage SBI’s high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors—available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The Cytosol Cyto-Tracer, pCT-Cyto-RFP (CMV), expresses an untagged RFP, which naturally localizes to the cytosol. RFP is expressed from the CMV promoter for strong expression in common cell types such as HeLa, HEK293, and HT1080.

• Stable lentivector-based system
• Great for creating stable reporter cell lines
• Ideal for co-localization studies
• Monitor cellular dynamics and functional studies in real time
• Label cells with RFP for a broad range of studies

Choose the Cyto-Tracer that’s right for you

How It Works

Visualizing the cell with Cyto-Tracers

SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location.

Supporting Data

See some of our Cyto-Tracers in action

Resources

Citations

• Kessler, M, et al. (2019) Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation. Nat Commun. 2019 Mar 18; 10(1):1194. PM ID: 30886143
• Gonzalez-King, H, et al. (2017) Hypoxia Inducible Factor-1α Potentiates Jagged 1-Mediated Angiogenesis by Mesenchymal Stem Cell-Derived Exosomes. Stem Cells. 2017 Jul 1; 35(7):1747-1759. PM ID: 28376567
• Jawhari, S, et al. (2017) Autophagy and TrkC/NT-3 signaling joined forces boost the hypoxic glioblastoma cell survival. Carcinogenesis. 2017 Jun 1; 38(6):592-603. PM ID: 28402394
• Braganza, A, et al. (2017) UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J. Biol. Chem.. 2017 Feb 10; 292(6):2470-2484. PM ID: 28003368
• Soares, VYR. (2017) O papel das vesículas extracelulares na fisiopatologia da perda auditiva ocasionada pelo Schwannoma vestibular. Thesis. ;. Link: Thesis
• Yoon, C, et al. (2016) Delivery of miR-155 to retinal pigment epithelial cells mediated by Burkitt’s lymphoma exosomes. Tumour Biol.. 2016 Jan 1; 37(1):313-21. PM ID: 26211004
• Lo Cicero, A, et al. (2015) Exosomes released by keratinocytes modulate melanocyte pigmentation. Nat Commun. 2015 Jun 24; 6:7506. PM ID: 26103923
• Jawhari, S. (2015) Etude des signalisations autophagique et neurotrophique dans des lignées de glioblastome humain activées lors de l’hypoxie. Thesis. ;. Link: Thesis
• VARLET, P, PU-PH, P & DJAVAHERI-MERGNY, M. (2015) SOHA JAWHARI. Thesis. ;. Link: Thesis
• Liang, Y. (2015) Exosome/Microvesicle Cargo Contents Recruitment in a Glycosylation-dependent Pathway. Thesis. ;. Link: Thesis
• Fang, Q, et al. (2014) HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β. Nat Commun. 2014 Nov 26; 5:5513. PM ID: 25423885
• Liang, Y, et al. (2014) Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J. Biol. Chem.. 2014 Nov 21; 289(47):32526-37. PM ID: 25261472
• Sato, Y, et al. (2014) MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane. Nat Commun. 2014 Nov 4; 5:5266. PM ID: 25366663