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pGreenFire 2.0 NFκB Reporter Lentivector & Virus

Study NF-κB signaling with the pGreenFire 2.0 NFκB Reporter (red firefly luciferase & GFP), which is engineered for reliable stable cell line generation.

  • Sort responsive cells with GFP
  • Measure activity with red firefly luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
Catalog Number
Description
Size
Price
Quantity
Add to Cart
TR412PA-P
pGreenFire 2.0 NFkB reporter plasmid (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro)
10 µg
$ 660
TR412VA-P
pGreenFire 2.0 NFkB reporter virus (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro)
>2 x10^6 IFUs
$ 660

Overview

Monitor signal transduction in real time with our re-engineered pGreenFire 2.0 Lentivectors

SBI has upgraded our popular pGreenFire signaling pathway reporter lentivectors with a design that leads to more reliable generation of stable cell lines. We’ve also swapped in the red firefly luciferase reporter (rFLuc), which opens up the possibility of performing a dual-spectral luciferase assay and delivers greater sensitivity for in vivo applications than conventional luciferase.

With the pGreenFire 2.0 NFκB Reporter Lentivector & Virus (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro), the core reporter functionality is similar to the original pGreenFire lentivector—NF-κB transcriptional response elements (TREs) are placed upstream of a minimal CMV promoter (mCMV) which together drive co-expression of rFLuc and GFP in response to NF-κB activity. The result is the ability to quantitatively measure NF-κB activity using both fluorescence and luciferase activity.

What makes our next-gen pGreenFire 2.0 vectors even better than other TRE reporter vectors is the smart design, which adds in a constitutive selection cassette for stable cell line generation while minimizing interference with the upstream TRE. By using a weak/moderate mPGK promoter to drive the antibiotic selection marker (puromycin resistance) and carefully arranging the conditional reporter genes, the selection marker is reliably expressed without compromising conditional expression of rFLuc and GFP.

As with our original pGreenFire1 vectors, all pGreenFire 2.0 lentivectors leverage our reliable lentivector technology and save you time with pre-built signal transduction pathway reporters that come as ready-to-transduce pre-packaged lentivirus and plasmid that can be transfected into the lentivirus producing system of your choice*.

  • Sort responsive cells with GFP
  • Measure activity with red firefly luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines

*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

Supporting Data

See our pGreenFire 2.0 transcriptional response element reporters in action

Figure 1. The pGreenFire 2.0 NFκB Reporter efficiently and quantitatively reports on NFκB activity in MDA-MB-213 cells. Relative luciferase activity (A) and GFP activity (B) both increase in response to TNFα, and NFκB inducer. (C) Like all pGreenFire 2.0 lentivectors, the pGreenFire 2.0 NFκB Reporter contains an mPGK-Puro cassette to streamline creation of stable reporters integrated into the cell lines of your choice.

Citations

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  • Cen, X, et al. (2019) TLR1/2 Specific Small‐Molecule Agonist Suppresses Leukemia Cancer Cell Growth by Stimulating Cytotoxic T Lymphocytes. Adv. Sci.. 2019 Mar 27;:1802042. Link: Adv. Sci.
  • Wang, J, et al. (2019) Siglec-15 as an immune suppressor and potential target for normalization cancer immunotherapy. Nat. Med.. 2019 Mar 4;. PM ID: 30833750
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  • Ma, Y, et al. (2019) Inhibition of pancreatic cancer stem cell characteristics by α-Mangostin: Molecular mechanisms involving Sonic hedgehog and Nanog. J. Cell. Mol. Med.. 2019 Feb 3;. PM ID: 30712329
  • Yuan, S, et al. (2019) SREBP-dependent lipidomic reprogramming as a broad-spectrum antiviral target. Nat Commun. 2019 Jan 10; 10(1):120. PM ID: 30631056
  • Lin, YS, et al. (2019) Liver X Receptor/Retinoid X Receptor Pathway Plays a Regulatory Role in Pacing-Induced Cardiomyopathy. J Am Heart Assoc. 2019 Jan 8; 8(1):e009146. PM ID: 30612502
  • Heun, Y, et al. (2017) HIF-1α Dependent Wound Healing Angiogenesis In Vivo Can Be Controlled by Site-Specific Lentiviral Magnetic Targeting of SHP-2. Mol. Ther.. 2017 Jul 5; 25(7):1616-1627. PM ID: 28434868
  • Phua, KKL, Liu, Y & Sim, SH. (2017) Non-linear enhancement of mRNA delivery efficiencies by influenza A derived NS1 protein engendering host gene inhibition property. Biomaterials. 2017 Jul 1; 133:29-36. PM ID: 28426973
  • Takada, S, et al. (2017) Pluripotent stem cell models of Blau syndrome reveal an IFN-γ-dependent inflammatory response in macrophages. J. Allergy Clin. Immunol.. 2017 Jun 3;. PM ID: 28587749
  • Li, X, et al. (2017) Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy. Mol. Cell. 2017 Jun 1; 66(5):684-697.e9. PM ID: 28552616
  • Chen, YC, et al. (2017) Selective Photomechanical Detachment and Retrieval of Divided Sister Cells from Enclosed Microfluidics for Downstream Analyses. ACS Nano. 2017 May 23; 11(5):4660-4668. PM ID: 28480715
  • Choi, EJ, et al. (2017) A clinical drug library screen identifies clobetasol propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer. Oncogene. 2017 May 15;. PM ID: 28504720
  • Krabbe, G, et al. (2017) Microglial NFκB-TNFα hyperactivation induces obsessive-compulsive behavior in mouse models of progranulin-deficient frontotemporal dementia. Proc. Natl. Acad. Sci. U.S.A.. 2017 May 9; 114(19):5029-5034. PM ID: 28438992
  • Schmidt, C, et al. (2017) Pre-clinical drug screen reveals topotecan, actinomycin D and volasertib as potential new therapeutic candidates for ETMR brain tumor patients. Neuro-oncology. 2017 May 8;. PM ID: 28482026
  • Pryke, KM, et al. (2017) A Novel Agonist of the TRIF Pathway Induces a Cellular State Refractory to Replication of Zika, Chikungunya, and Dengue Viruses. MBio. 2017 May 2; 8(3). PM ID: 28465426
  • Gryder, BE, et al. (2017) PAX3-FOXO1 Establishes Myogenic Super Enhancers and Confers BET Bromodomain Vulnerability. Cancer Discov. 2017 Apr 26;. PM ID: 28446439
  • Jin, C, et al. (2017) Over-expression of ASIC1a promotes proliferation via activation of the β-catenin/LEF-TCF axis and is associated with disease outcome in liver cancer. Oncotarget. 2017 Apr 18; 8(16):25977-25988. PM ID: 27462920
  • Wang, W, et al. (2017) Biological or pharmacological activation of protein kinase C alpha constrains hepatitis E virus replication. Antiviral Res.. 2017 Apr 1; 140:1-12. PM ID: 28077314
  • Teng, CT, et al. (2017) Development of Novel Cell Lines for High-Throughput Screening to Detect Estrogen-Related Receptor Alpha Modulators. SLAS Discov. 2017 Jan 1;:2472555216689772. PM ID: 28346099

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