pGreenFire Control Pack

Run your pGreenFire projects with confidence with all of the positive and negative pGreenFire1 controls bundled together

Products

Catalog Number Description Size Price Quantity Add to Cart
TR000PA-1 pGreenFire Control Pack- pGreenFire negative and positive control plasmids, only available with purchase of other pGF reporters 10 µg Each $386
- +

Overview

Overview

Supporting your studies with ready-to-go controls

No need to make positive or negative controls for your pGreenFire projects—SBI’s already got them built. With the pGreenFire Control Pack (available only with the purchase of any of our pGreenFire Reporter), you get the pGreenFire1-mCMV Negative Control (Cat.# TR010PA/VA-1), with both EF1α-neo and EF1α-puro versions (Cat.#s TR010PA/VA-N and Cat.# TR010PA/VA-P), and the pGreenFire1-CMV Positive Control Lentivector (Cat.# TR011PA/VA-1).

All of these vectors feature the GreenFire cassette—a destabilized copepod GFP (dscGFP; 2-hour half-life) co-expressed with luciferase. The negative controls contain only the minimal CMV promoter (mCMV) and deliver negligible expression, whereas the positive control includes the full CMV promoter for strong expression in common cell types, such as HeLa, HEK293, and HT1080.

pGreenFire1-CMV Positive Control LentivectorpGreenFire1-mCMV Negative Control LentivectorpGreenFire1-mCMV (EF1α-neo) Negative Control LentivectorpGreenFire1-mCMV (EF1α-puro) Negative Control Lentivector

How It Works

Supporting Data

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cellsTrack and measure the activity of oncogenic signal transduction pathways in live cells

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examplesSee SBI’s pGreenFire1 reporters in action

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in actionSee SBI’s pGreenFire1 reporters in action

FAQs

Resources

Citations

  • Gampala, S, et al. (2024) New Ref-1/APE1 targeted inhibitors demonstrating improved potency for clinical applications in multiple cancer types. Pharmacological Research. 2024;:107092. Link: Pharmacological Research
  • Liu, YN, et al. (2024) Immunosuppressive role of BDNF in therapy-induced neuroendocrine prostate cancer. Molecular oncology. 2024;. PM ID: 38381121
  • Ishino, T, et al. (2023) Somatic mutations can induce a noninflamed tumour microenvironment via their original gene functions, despite deriving neoantigens. British journal of cancer. 2023;. PM ID: 36732592
  • Pandi, K, et al. (2023) Porphyromonas gingivalis induction of TLR2 association with Vinculin enables PI3K activation and immune evasion. PLoS pathogens. 2023; 19(4):e1011284. PM ID: 37023213
  • Ramachandran, M, et al. (2023) Tailoring vascular phenotype through AAV therapy promotes anti-tumor immunity in glioma. Cancer cell. 2023;. PM ID: 37172581
  • Wen, YC, et al. (2023) CHRM4/AKT/MYCN upregulates interferon alpha-17 in the tumor microenvironment to promote neuroendocrine differentiation of prostate cancer. Cell death & disease. 2023; 14(5):304. PM ID: 37142586
  • Li, X, et al. (2023) Rosmarinic acid ameliorates autoimmune responses through suppression of intracellular nucleic acid-mediated type I interferon expression. Biochemical and Biophysical Research Communications. 2023;. Link: Biochemical and Biophysical Research Communications
  • Ibrahim, L, et al. (2023) Succinylation of a KEAP1 sensor lysine promotes NRF2 activation. bioRxiv : the preprint server for biology. 2023;. PM ID: 37215033
  • Park, CS, et al. (2023) Stromal-induced epithelial-mesenchymal transition induces targetable drug resistance in acute lymphoblastic leukemia. Cell reports. 2023; 42(7):112804. PM ID: 37453060
  • Ouyang, W, et al. (2023) Development of a New Cell-Based AP-1 Gene Reporter Potency Assay for Anti-Anthrax Toxin Therapeutics. Toxins. 2023; 15(9):528. Link: Toxins
  • Zhao, G, et al. (2023) Base editing of the mutated TERT promoter inhibits liver tumor growth. Hepatology (Baltimore, Md.). 2023;. PM ID: 38016019
  • You, S & Bollong, MJ. (2023) A high throughput screen for pharmacological inhibitors of the carbohydrate response element. Scientific data. 2023; 10(1):676. PM ID: 37794069
  • Melo, CL. (2023) LUMINALABREASTCANCER: INSIGHTS INTOCELLCYCLEREGULATIONAND ESTROGENSIGNALING. Thesis. 2023;. Link: Thesis
  • Labanieh, L, et al. (2022) Enhanced safety and efficacy of protease-regulated CAR-T cell receptors. Cell. 2022;. PM ID: 35483375
  • Teng, CT, et al. (2022) SUPPLEMENTARY MATERIAL: Development of novel cell lines for high throughput screening to detect estrogen-related receptor alpha modulators. slas-discovery.org. 2022;. Link: slas-discovery.org
  • Dane, EL, et al. (2022) STING agonist delivery by tumour-penetrating PEG-lipid nanodiscs primes robust anticancer immunity. Nature materials. 2022; 21(6):710-720. PM ID: 35606429
  • Liu, Y, et al. (2022) MCTP1 promotes SNAI1-driven neuroendocrine differentiation and epithelial-to- mesenchymal transition of prostate cancer enhancement by ZBTB46/FOXA2/HIF1A. Research Square. 2022;. Link: Research Square
  • Deng, Z, Lyu, W & Zhang, G. (2022) High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression. Antibiotics (Basel, Switzerland). 2022; 11(7). PM ID: 35884187
  • Chang, WM, et al. (2022) The aberrant cancer metabolic gene carbohydrate sulfotransferase 11 promotes non-small cell lung cancer cell metastasis via dysregulation of ceruloplasmin and intracellular iron balance. Translational oncology. 2022; 25:101508. PM ID: 35985204
  • Chen, C, et al. (2022) ATF4-dependent fructolysis fuels growth of glioblastoma multiforme. Nature communications. 2022; 13(1):6108. PM ID: 36245009

Products

Catalog Number Description Size Price Quantity Add to Cart
TR000PA-1 pGreenFire Control Pack- pGreenFire negative and positive control plasmids, only available with purchase of other pGF reporters 10 µg Each $386
- +

Overview

Overview

Supporting your studies with ready-to-go controls

No need to make positive or negative controls for your pGreenFire projects—SBI’s already got them built. With the pGreenFire Control Pack (available only with the purchase of any of our pGreenFire Reporter), you get the pGreenFire1-mCMV Negative Control (Cat.# TR010PA/VA-1), with both EF1α-neo and EF1α-puro versions (Cat.#s TR010PA/VA-N and Cat.# TR010PA/VA-P), and the pGreenFire1-CMV Positive Control Lentivector (Cat.# TR011PA/VA-1).

All of these vectors feature the GreenFire cassette—a destabilized copepod GFP (dscGFP; 2-hour half-life) co-expressed with luciferase. The negative controls contain only the minimal CMV promoter (mCMV) and deliver negligible expression, whereas the positive control includes the full CMV promoter for strong expression in common cell types, such as HeLa, HEK293, and HT1080.

pGreenFire1-CMV Positive Control LentivectorpGreenFire1-mCMV Negative Control LentivectorpGreenFire1-mCMV (EF1α-neo) Negative Control LentivectorpGreenFire1-mCMV (EF1α-puro) Negative Control Lentivector

How It Works

Supporting Data

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cellsTrack and measure the activity of oncogenic signal transduction pathways in live cells

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examplesSee SBI’s pGreenFire1 reporters in action

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in actionSee SBI’s pGreenFire1 reporters in action

FAQs

Citations

  • Gampala, S, et al. (2024) New Ref-1/APE1 targeted inhibitors demonstrating improved potency for clinical applications in multiple cancer types. Pharmacological Research. 2024;:107092. Link: Pharmacological Research
  • Liu, YN, et al. (2024) Immunosuppressive role of BDNF in therapy-induced neuroendocrine prostate cancer. Molecular oncology. 2024;. PM ID: 38381121
  • Ishino, T, et al. (2023) Somatic mutations can induce a noninflamed tumour microenvironment via their original gene functions, despite deriving neoantigens. British journal of cancer. 2023;. PM ID: 36732592
  • Pandi, K, et al. (2023) Porphyromonas gingivalis induction of TLR2 association with Vinculin enables PI3K activation and immune evasion. PLoS pathogens. 2023; 19(4):e1011284. PM ID: 37023213
  • Ramachandran, M, et al. (2023) Tailoring vascular phenotype through AAV therapy promotes anti-tumor immunity in glioma. Cancer cell. 2023;. PM ID: 37172581
  • Wen, YC, et al. (2023) CHRM4/AKT/MYCN upregulates interferon alpha-17 in the tumor microenvironment to promote neuroendocrine differentiation of prostate cancer. Cell death & disease. 2023; 14(5):304. PM ID: 37142586
  • Li, X, et al. (2023) Rosmarinic acid ameliorates autoimmune responses through suppression of intracellular nucleic acid-mediated type I interferon expression. Biochemical and Biophysical Research Communications. 2023;. Link: Biochemical and Biophysical Research Communications
  • Ibrahim, L, et al. (2023) Succinylation of a KEAP1 sensor lysine promotes NRF2 activation. bioRxiv : the preprint server for biology. 2023;. PM ID: 37215033
  • Park, CS, et al. (2023) Stromal-induced epithelial-mesenchymal transition induces targetable drug resistance in acute lymphoblastic leukemia. Cell reports. 2023; 42(7):112804. PM ID: 37453060
  • Ouyang, W, et al. (2023) Development of a New Cell-Based AP-1 Gene Reporter Potency Assay for Anti-Anthrax Toxin Therapeutics. Toxins. 2023; 15(9):528. Link: Toxins
  • Zhao, G, et al. (2023) Base editing of the mutated TERT promoter inhibits liver tumor growth. Hepatology (Baltimore, Md.). 2023;. PM ID: 38016019
  • You, S & Bollong, MJ. (2023) A high throughput screen for pharmacological inhibitors of the carbohydrate response element. Scientific data. 2023; 10(1):676. PM ID: 37794069
  • Melo, CL. (2023) LUMINALABREASTCANCER: INSIGHTS INTOCELLCYCLEREGULATIONAND ESTROGENSIGNALING. Thesis. 2023;. Link: Thesis
  • Labanieh, L, et al. (2022) Enhanced safety and efficacy of protease-regulated CAR-T cell receptors. Cell. 2022;. PM ID: 35483375
  • Teng, CT, et al. (2022) SUPPLEMENTARY MATERIAL: Development of novel cell lines for high throughput screening to detect estrogen-related receptor alpha modulators. slas-discovery.org. 2022;. Link: slas-discovery.org
  • Dane, EL, et al. (2022) STING agonist delivery by tumour-penetrating PEG-lipid nanodiscs primes robust anticancer immunity. Nature materials. 2022; 21(6):710-720. PM ID: 35606429
  • Liu, Y, et al. (2022) MCTP1 promotes SNAI1-driven neuroendocrine differentiation and epithelial-to- mesenchymal transition of prostate cancer enhancement by ZBTB46/FOXA2/HIF1A. Research Square. 2022;. Link: Research Square
  • Deng, Z, Lyu, W & Zhang, G. (2022) High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression. Antibiotics (Basel, Switzerland). 2022; 11(7). PM ID: 35884187
  • Chang, WM, et al. (2022) The aberrant cancer metabolic gene carbohydrate sulfotransferase 11 promotes non-small cell lung cancer cell metastasis via dysregulation of ceruloplasmin and intracellular iron balance. Translational oncology. 2022; 25:101508. PM ID: 35985204
  • Chen, C, et al. (2022) ATF4-dependent fructolysis fuels growth of glioblastoma multiforme. Nature communications. 2022; 13(1):6108. PM ID: 36245009