pGreenFire1-mCMV (EF1α-neo) Negative Control Lentivector

Overview

Supporting your studies with ready-to-go controls

No need to make a negative control for your pGreenFire projects—SBI’s already built one for you. With the pGreenFire1-mCMV (EF1α-neo) Negative Control Lentivector, the GreenFire cassette is driven by a minimal CMV promoter with destabilized copepod GFP (dscGFP, 2-hour half-life) and luciferase co-expression mediated by a T2A element. The minimal CMV promoter delivers negligible expression, and this configuration provides a control for background levels of GFP and luciferase expression in the absence of enhancer elements, such as the ones used in our Signal Transduction Pathway Reporters/Transcriptional Response Element Reporters.

To simplify cell line construction, this pGreenFire1-mCMV (EF1α-neo) Negative Control Lentivector comes with a constitutively expressed neomycin marker expressed from the Ef1α promoter. Or choose an EF1α-puro (Cat.# TR010PA-P), or no marker at all (Cat.# TR010PA-1). All versions of this lentivector are available as plasmid or pre-packaged virus.

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examples

Monitoring NF-κB transactivation

Resources

Citations

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• Pryke, KM, et al. (2017) A Novel Agonist of the TRIF Pathway Induces a Cellular State Refractory to Replication of Zika, Chikungunya, and Dengue Viruses. MBio. 2017 May 2; 8(3). PM ID: 28465426
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