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pLL-CMV-GFP-T2A-Puro Lenti-Labeler™ Lentivector Plasmid & Pre-packaged Virus

Reliably label the cells of your choice with copGFP driven by the strong CMV promoter (and with puro selection) – great for tracking cells, HTS assays, and more

  • Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
  • Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
  • Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)
Catalog Number
Add to Cart
10 µg
$ 575
>2 x10^6 IFUs
$ 575


Reliable cell labeling, delivered

SBI’s family of Lenti-Labeler™ constructs facilitate a wide range of studies—including cell tracking, high-throughput assays, and more—by enabling efficient and reliable labeling of your cells. The pLL-CMV-GFP-T2A-Puro Lenti-Labeler™ construct expresses copepod GFP from the CMV promoter, which delivers strong expression in most commonly-used cell lines (HeLa, HEK293, HT-1080, etc.), and co-expresses the puromycin resistance gene for selection in vitro prior to in vivo use. Available as either fully propagatable, sequence-verified plasmid DNA (Cat.# LL100A-1) or ready-to-transduce pre-packaged lentivirus (Cat.# LL100VA-1), SBI’s pLL-CMV-GFP-T2A-Puro Lenti-Labeler™ is designed for reliability, so you can get to valuable insights faster.

  • Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
  • Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
  • Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)

While the pLL-CMV-GFP-T2A-Puro Lenti-Labeler construct is compatible with most second and third generation packaging plasmid mixes, SBI recommends the use of pPACKH1 (Cat #LV500A-1) and TransDux MAX Transduction Reagent (Cat #LV860A-1) to achieve optimal virus titers and infection of target cells.

Find the right Lenti-Labeler construct for your studies

SBI offers a Lenti-Labeler constructs with a range of selection markers, reporter genes, and two different promoters.

Table 1. Available Lenti-Labeler Constructs

Cat. # Construct Promoter Reporter Selection
LL100PA-1/LL100VA-1 pLL-CMV-GFP-T2A-Puro CMV GFP Puromycin
LL105PA-1/LL105VA-1 pLL-CMV-GFP-T2A-Blast

CMV GFP Blasticidin
LL110PA-1/LL110VA-1 pLL-CMV-RFP-T2A-Puro CMV RFP Puromycin
LL115PA-1/LL115VA-1 pLL-CMV-RFP-T2A-Blast CMV RFP Blasticidin
LL120PA-1/LL120VA-1 pLL-CMV-BFP-T2A-Puro CMV BFP Puromycin
LL125PA-1/LL125VA-1 pLL-CMV-BFP-T2A-Blast CMV BFP Blasticidin
LL150PA-1/LL150VA-1 pLL-CMV-Luciferase-T2A-Puro CMV Luciferase Puromycin
LL200PA-1/LL200VA-1 pLL-EF1α-GFP-T2A-Puro EF1α GFP Puromycin
LL205PA-1/LL205VA-1 pLL-EF1α-GFP-T2A-Blast EF1α GFP Blasticidin
LL210PA-1/LL210VA-1 pLL-EF1α-RFP-T2A-Puro EF1α RFP Puromycin
LL215PA-1/LL215VA-1 pLL-EF1α-RFP-T2A-Blast EF1α RFP Blasticidin
LL220PA-1/LL220VA-1 pLL-EF1α-BFP-T2A-Puro EF1α BFP Puromycin
LL225PA-1/LL225VA-1 pLL-EF1α-BFP-T2A-Blast EF1α BFP Blasticidin
LL250PA-1/LL250VA-1 pLL-EF1α-Luciferase-T2A-Puro EF1α Luciferase Puromycin
LL300PA-1/LL300VA-1 pLL-CMV-rFLuc-T2A-GFP CMV Luciferase & GFP N/A
LL310PA-1/LL310VA-1 pLL-CMV-rFLuc-T2A-GFP-mPGK-Puro CMV Luciferase & GFP Puromycin
LL320PA-1/LL320VA-1 pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro CMV Luciferase & RFP Puromycin
LL410PA-1/LL410VA-1 pLL-EF1a-rFLuc-T2A-GFP-mPGK-Puro EF1α Luciferase & GFP Puromycin

pLL-EF1a-rFLuc-T2A-mRFP-mPGK-Puro EF1α Luciferase & RFP Puromycin

Supporting Data

SBI’s Lenti-Labelers get your cells labeled efficiently, reliably

Figure 1. SBI Lenti-Labeler constructs reliably and efficiently label cells. Comparison of the number of fluorescently-labeled cells to the total number of cells seen in the corresponding phase contrast images reveals the high labeling efficiency of SBI’s Lenti-Labeler constructs.


  • Winston, C, et al. (2019) Complement protein levels in plasma astrocyte-derived exosomes are abnormal in conversion from mild cognitive impairment to Alzheimer’s disease dementia. Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring. 2019 Dec 1; 11:61-66. Link: Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring
  • Kim, Y & Mok, H. (2019) Citraconylated exosomes for improved internalization into macrophages. Appl Biol Chem. 2019 Dec 1; 62(1). Link: Appl Biol Chem
  • Saunders, CA, et al. (2019) Genetic manipulation of PLB-985 cells and quantification of chemotaxis using the underagarose assay. Methods Cell Biol.. 2019 Oct 29; 149:31-56. PM ID: 30616826
  • Sekiba, K, et al. (2019) Inhibition of HBV Transcription From cccDNA With Nitazoxanide by Targeting the HBx-DDB1 Interaction. Cell Mol Gastroenterol Hepatol. 2019 Oct 24; 7(2):297-312. PM ID: 30704981
  • Yang, JS, et al. (2019) Investigation of lipidomic perturbations in oxidatively stressed subcellular organelles and exosomes by asymmetrical flow field-flow fractionation and nanoflow ultrahigh performance liquid chromatography-tandem mass spectrometry. Anal. Chim. Acta. 2019 Sep 27; 1073:79-89. PM ID: 31146839
  • Ishihara, R, et al. (2019) Design of a surface-functionalized power-free microchip for extracellular vesicle detection utilizing UV grafting. Reactive and Functional Polymers. 2019 Sep 1; 142:183-188. Link: Reactive and Functional Polymers
  • Gamboa, L, et al. (2019) Heat-triggered remote control of CRISPR-dCas9 for tunable transcriptional modulation. bioRxiv. 2019 Aug 14;. Link: bioRxiv
  • Xu, Q, et al. (2019) miR‑132 inhibits high glucose‑induced vascular smooth muscle cell proliferation and migration by targeting E2F5. Mol Med Rep. 2019 Aug 1; 20(2):2012-2020. PM ID: 31257477
  • Liu, L, et al. (2019) IL‑17A promotes CXCR2‑dependent angiogenesis in a mouse model of liver cancer. Mol Med Rep. 2019 Aug 1; 20(2):1065-1074. PM ID: 31173199
  • Hisano, Y, et al. (2019) Lysolipid receptor cross-talk regulates lymphatic endothelial junctions in lymph nodes. J. Exp. Med.. 2019 Jul 1; 216(7):1582-1598. PM ID: 31147448
  • Choi, YW, et al. (2019) Abrogation of B-RafV600E induced senescence by FoxM1 expression. Biochem. Biophys. Res. Commun.. 2019 Jun 30;. PM ID: 31270027
  • Madhu, LN, et al. (2019) Neuroinflammation in Gulf War Illness is linked with HMGB1 and complement activation, which can be discerned from brain-derived extracellular vesicles in the blood. Brain Behav. Immun.. 2019 Jun 27;. PM ID: 31255677
  • Amado, F, et al. (2019) Sample Treatment for Saliva Proteomics. Adv. Exp. Med. Biol.. 2019 Jun 26; 1073:23-56. PM ID: 31236838
  • Tian, L, et al. (2019) Long-read sequencing unveils IGH-DUX4 translocation into the silenced IGH allele in B-cell acute lymphoblastic leukemia. Nat Commun. 2019 Jun 26; 10(1):2789. PM ID: 31243274
  • Chen, CY, et al. (2019) Extracellular vesicles from human urine-derived stem cells prevent osteoporosis by transferring CTHRC1 and OPG. Bone Res. 2019 Jun 26; 7:18. PM ID: 31263627
  • Zhang, L & Li, D. (2019) MORC2 regulates DNA damage response through a PARP1-dependent pathway. Nucleic Acids Research. 2019 Jun 25;. Link: Nucleic Acids Research
  • Ariyoshi, K, et al. (2019) Radiation-Induced Bystander Effect is Mediated by Mitochondrial DNA in Exosome-Like Vesicles. Sci Rep. 2019 Jun 24; 9(1):9103. PM ID: 31235776
  • Lee, H, et al. (2019) Caveolin-1 selectively regulates microRNA sorting into microvesicles after noxious stimuli. J. Exp. Med.. 2019 Jun 24;. PM ID: 31235510
  • Zhao, J, et al. (2019) Tumor-Derived Extracellular Vesicles Inhibit Natural Killer Cell Function in Pancreatic Cancer. Cancers (Basel). 2019 Jun 22; 11(6). PM ID: 31234517
  • Wang, X, et al. (2019) Prostate carcinoma cell-derived exosomal MicroRNA-26a modulates the metastasis and tumor growth of prostate carcinoma. Biomed. Pharmacother.. 2019 Jun 20; 117:109109. PM ID: 31229922

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