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pLL-EF1a-BFP-T2A-Puro Lenti-Labeler™ Lentivector Plasmid & Pre-packaged Virus

Reliably label cells with BFP driven by a moderate EF1α promoter (and with puro selection) – great for tracking cells, HTS assays, and more

  • Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
  • Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
  • Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)
Catalog Number
Description
Size
Price
Quantity
Add to Cart
LL220PA-1
pLL-EF1a-BFP-T2A-Puro (Lenti-Labeler™ plasmid)
10 µg
$ 575
LL220VA-1
pLL-EF1a-BFP-T2A-Puro (Lenti-Labeler™ virus)
>2 x10^6 IFUs
$ 575

Overview

Reliable cell labeling, delivered

SBI’s family of Lenti-Labeler™ constructs facilitate a wide range of studies—including cell tracking, high-throughput assays, and more—by enabling efficient and reliable labeling of your cells. The pLL-EF1α-BFP-T2A-Puro Lenti-Labeler™ construct expresses BFP from the EF1α promoter, which delivers moderate expression in most cell types (including primary and stem cells), and co-expresses the puromycin resistance gene for selection in vitro prior to in vivo use. Available as either fully propagatable, sequence-verified plasmid DNA (Cat.# LL220PA-1) or ready-to-transduce pre-packaged lentivirus (Cat.# LL220VA-1), SBI’s pLL-EF1α-BFP-T2A-Puro Lenti-Labeler™ is designed for reliability, so you can get to valuable insights faster

  • Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
  • Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
  • Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)

While the pLL-EF1α-BFP-T2A-Puro Lenti-Labeler construct is compatible with most second and third generation packaging plasmid mixes, SBI recommends the use of pPACKH1 (Cat #LV500A-1) and TransDux MAX Transduction Reagent (Cat #LV860A-1) to achieve optimal virus titers and infection of target cells.

Find the right Lenti-Labeler construct for your studies

SBI offers Lenti-Labeler constructs with a range of selection markers, reporter genes, and two different promoters.

Table 1. Available Lenti-Labeler Constructs

Cat. # Construct Promoter Reporter Selection
LL100PA-1/LL100VA-1 pLL-CMV-GFP-T2A-Puro CMV GFP Puromycin
LL105PA-1/LL105VA-1 pLL-CMV-GFP-T2A-Blast

CMV GFP Blasticidin
LL110PA-1/LL110VA-1 pLL-CMV-RFP-T2A-Puro CMV RFP Puromycin
LL115PA-1/LL115VA-1 pLL-CMV-RFP-T2A-Blast CMV RFP Blasticidin
LL120PA-1/LL120VA-1 pLL-CMV-BFP-T2A-Puro CMV BFP Puromycin
LL125PA-1/LL125VA-1 pLL-CMV-BFP-T2A-Blast CMV BFP Blasticidin
LL150PA-1/LL150VA-1 pLL-CMV-Luciferase-T2A-Puro CMV Luciferase Puromycin
LL200PA-1/LL200VA-1 pLL-EF1α-GFP-T2A-Puro EF1α GFP Puromycin
LL205PA-1/LL205VA-1 pLL-EF1α-GFP-T2A-Blast EF1α GFP Blasticidin
LL210PA-1/LL210VA-1 pLL-EF1α-RFP-T2A-Puro EF1α RFP Puromycin
LL215PA-1/LL215VA-1 pLL-EF1α-RFP-T2A-Blast EF1α RFP Blasticidin
LL220PA-1/LL220VA-1 pLL-EF1α-BFP-T2A-Puro EF1α BFP Puromycin
LL225PA-1/LL225VA-1 pLL-EF1α-BFP-T2A-Blast EF1α BFP Blasticidin
LL250PA-1/LL250VA-1 pLL-EF1α-Luciferase-T2A-Puro EF1α Luciferase Puromycin
LL300PA-1/LL300VA-1 pLL-CMV-rFLuc-T2A-GFP CMV Luciferase & GFP N/A
LL310PA-1/LL310VA-1 pLL-CMV-rFLuc-T2A-GFP-mPGK-Puro CMV Luciferase & GFP Puromycin
LL320PA-1/LL320VA-1 pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro CMV Luciferase & RFP Puromycin
LL410PA-1/LL410VA-1 pLL-EF1a-rFLuc-T2A-GFP-mPGK-Puro EF1α Luciferase & GFP Puromycin
LL420PA-1/LL420VA-1

pLL-EF1a-rFLuc-T2A-mRFP-mPGK-Puro EF1α Luciferase & RFP Puromycin

Supporting Data

SBI’s Lenti-Labelers get your cells labeled efficiently, reliably

Figure 1. SBI Lenti-Labeler constructs reliably and efficiently label cells. Comparison of the number of fluorescently-labeled cells to the total number of cells seen in the corresponding phase contrast images reveals the high labeling efficiency of SBI’s Lenti-Labeler constructs.

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