QuantiMir™ Human U6 Reference Assays
Extend your QuantiMir Kit with additional human U6 reference assays.
- Ready-to-use reference assay
- Designed for use with the QuantiMir Kit
- Suitable for high-throughput screening of clinical samples
Supporting your studies with ready-to-go reference assays
QuantiMir™ is SBI’s simple and robust technology for profiling miRNAs. For labs using our QuantiMir Kit who need additional human U6 reference assays, we offer this stand-alone set of 50 assays.
Visit the QuantiMir page to order the complete kit.
See all of our reference assays:
|Catalog #||Amplified small RNA||Species compatibility|
|RA420A-RNU1||RNU1 (U1)||Human, Mouse, Rat, Monkey|
|RA420A-RNU43||RNU43||Human, Mouse, Rat|
How the QuantiMir Kit Works
The QuantiMir Kit enables robust miRNA quantitation through a simple and efficient workflow
Highly efficient poly-A tailing and reverse transcription in a single reaction tube provides uniform cDNA synthesis of miRNAs. The optimized reaction conditions and buffer components maximize cDNA yield when starting with several micrograms down to picograms of input total RNA. The universal 3′ tag sequence incorporated during reverse transcription enables easily scalable and accurate miRNA expression analysis by qPCR—profile thousands of different miRNAs from a single reverse transcription reaction.
- Tag all small RNAs with a poly-A tail
- Anneal an oligo-dT adaptor
- Reverse transcribe to create first-strand cDNA
The result is a cDNA pool of anchor-tailed miRNAs that are ready for qPCR.
Create custom miRNA assays
To design your own miRNA assays, simply synthesize an oligo using the sequence of the mature miRNA you’d like to profile as the forward primer in your miRNA qPCR assay, and use with the universal reverse primer included in the QuantiMir Kit.
Supporting Data for the QuantiMir Kit
Converting miRNA into cDNA for accurate qPCR profiling and quantitation
The QuantiMir Kit is sensitive
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