Home | Products | Exosome Research | Exosome Isolation | SmartSEC™ Single for EV Isolation

SmartSEC™ Single for EV Isolation

Achieve EV isolation with the high purity and yield of SBI’s SmartSEC size exclusion-based technology in a single tube format

  • Quick and easy isolation
  • Better purity and yield than ultracentrifugation
  • Higher EV concentration per fraction than competitors’ conventional size exclusion chromatography kits
  • Validated for human serum, plasma, and CSF as well as Aplysia californica hemolymph
  • Compatible with most downstream applications such as mass spectrometry, western blotting, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) and RNA-sequencing (NGS)
Catalog Number
Description
Size
Price
Quantity
Add to Cart
SSEC200A-1
SmartSEC Single EV Isolation System
10 Reactions
$ 375

Get outstanding SEC-based EV isolation with SmartSEC™ Single

SmartSEC Single uses the same proprietary chromatography-based extracellular vesicle (EV) isolation technology as our popular SmartSEC HT plate and SmartSEC Mini kit but in a single tube format. SmartSEC technology combines all the benefits of size exclusion chromatography (SEC)—purity, yield, reproducibility, and preservation of EV integrity—with a contaminant trapping feature that enhances the capabilities of conventional SEC. The result is best-in-class EV isolation that is fast, easy, and clean.

  • Quick and easy isolation
  • Better purity and yield than ultracentrifugation
  • Higher EV concentration per fraction than competitors’ conventional size exclusion chromatography kits
  • Validated for human serum, plasma, and CSF as well as Aplysia californica hemolymph
  • Compatible with most downstream applications such as mass spectrometry, western blotting, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) and RNA-sequencing (NGS)

Each SmartSEC Single Kit comes with sufficient individual SmartSEC columns and buffer to process 10 samples.

How it works

The SmartSEC Single workflow is fast and easy. Simply apply 100 – 250 µL of cleared serum or plasma with additional column buffer or up to 4 mL of other biofluids directly to the pre-washed column, incubate, and centrifuge to elute the EVs.

SmartSEC Single performs better than competitor’s q SEC technology

To understand how well SmartSEC Single performs compared to a competitor’s q SEC columns, we isolated EVs from 250 µL of human serum using both SmartSEC Single and q SEC columns and analyzed 1 µg of protein equivalent from each isolation method on a western blot (Figure 1) and using fluorescent nanoparticle tracking analysis (fNTA, Figure 2). EVs isolated using SmartSEC Single show lower levels of carry-over proteins such as albumin and IgGs (Figure 1) and a higher number of particles per mg of protein (Figure 2) than EVs isolated using the q SEC columns, demonstrating the higher purity delivered by SmartSEC Single.

In addition, SmartSEC Single delivered EVs at ~6-fold higher concentration than the q SEC columns (Figure 1), for overall superior performance.

Figure 1. Western blot analysis shows that SmartSEC Single delivers higher yields of cleaner EVs than a competitor’s q SEC columns.

Figure 2. fNTA show that SmartSEC Single delivers higher yields of cleaner EVs than a competitor’s q SEC column.

SmartSEC Single cleanly isolates EVs from CSF

SmartSEC Single can isolate EVs from cerebrospinal fluid (CSF) with no detectable cellular contamination, as shown in the ExoCheck Neuro Array in Figure 3. The isolated EVs possess both neuronal and exosomal markers, suggesting that the EV prep is enriched for vesicles of neuronal origin. Interestingly, EVs isolated from CSF tend to be smaller in size than EVs isolated from serum (Figure 4).

Figure 3. SmartSEC Single isolates neuronal EVs from CSF.

Figure 4. EVs isolated from CSF by SmartSEC Singles are smaller than EVs isolated from serum.

EVs isolated using SmartSEC Single possess typical EV morphology

Transmission electron microscopy (TEM) of EVs isolated from serum using SmartSEC Single possess typical EV morphology—intact vesicles with a double layer of membranes—with little visible background debris (Figure 5).

Figure 5. EVs isolated using SmartSEC Single possess typical EV morphology.

EVs isolated with SmartSEC Single and a competitor’s q SEC columns have very similar proteomic profiles

We analyzed EVs isolated from human serum using SmartSEC Single and a competitor’s q SEC columns using mass spectrometry. Both methods yielded very similar proteomes, with 86% overlap (Figure 6).

Figure 6. EVs isolated with SmartSEC Single and a competitor’s q SEC columns have very similar proteomic profiles

SmartSEC Single can be used to isolate EVs for downstream proteomic studies

To demonstrate the versatility and power of SmartSEC Single for enabling EV research, we used SmartSEC Single technology to isolate EVs from normal serum and serum from prostate cancer patients and used mass spectrometry to analyze the respective proteomes. A selection of identified proteins is shown in the table below, and the full list can be downloaded from here.

UniProt Accession Number
Protein
Peptide count:
serum from healthy donors
Peptide count:
serum from donors with prostate cancer
P04275
von Willebrand factor; vWF; Contains: von Willebrand antigen 2; von Willebrand antigen II; Flags: Precursor;
1
259
A2NUT2
Lambda-chain (AA -20 to 215) {ECO:0000313|EMBL:CAA32725.1}; Flags: ...
209
110
B2RMS9
Inter-alpha (Globulin) inhibitor H4 (Plasma Kallikrein-sensitive ...
25
206
Q08380
Galectin-3-binding protein; Basement membrane autoantigen p105; ...
144
135
O43866
CD5 antigen-like; Apoptosis inhibitor expressed by macrophages ...
183
73
P07225
Vitamin K-dependent protein S; Flags: Precursor;
3
258
P00450
Ceruloplasmin; EC 1.16.3.1; Ferroxidase; Flags: Precursor;
91
110
Q1HP67
Lipoprotein, Lp(A) {ECO:0000313|EMBL:ABF47086.1};
1
124
P02790
Hemopexin; Beta-1B-glycoprotein; Flags: Precursor;
63
23
P07996
Thrombospondin-1; Glycoprotein G {ECO:0000303|PubMed:6777381}; ...
61

Have Questions?

A System Biosciences technical expert is happy to help!

(888) 266-5066 or Contact Us

Sign up to receive technical advice and exclusive deals directly to your inbox.