XStamp™-BHP1 Lentivector for Targeting Exosomes to Brain Cells

Target exosomes to brain cells with the pre-built XStamp-BHP1 Lentivector Construct.
  • Display BHP1 sequences on exosome surfaces
  • Target brain cells
  • Create stable XStamp-BHP1 cell lines

Products

Catalog Number Description Size Price Quantity Add to Cart
XSTP722PA-1 XStamp BHP1 brain targeting lentivector - uses BHP1 to target exosomes to the brain 10 µg $787
- +

Overview

Overview

Putting exosomes to work: Target exosomes to brain cells Use exosomes to deliver protein, RNA, DNA, or small molecule cargo to brain cells with XStamp-BHP1. The patented XStamp technology is an optimized, exosome surface display system that efficiently places protein sequences on exosomal surfaces. The XStamp-BHP1 Lentivector fuses the BHP1 peptide to the XStamp tag, ensuring that exosomes display BHP1 on their outer surface. Exosomes made from cells transfected or transduced with the XStamp-BHP1 Lentivector will now be targeted to brain cells.
  • Display BHP1 sequences on exosome surfaces
  • Target brain cells
  • Create stable XStamp-BHP1 cell lines
XStamp-BHP1 places BHP1 on the surface of your exosomes, targeting them to brain cells SBI also offers a selection of pre-built targeting XStamp Lentivectors, as well as a clone-your-own XStamp Cloning and Expression Vector:
Cat.#SpecificityTargeting Ligand Used
XSTP710PA-1Clone-your-ownYou decide
XSTP720PA-1GI tract cellsMotilin
XSTP721PA-1Neural cellsNCAM
XSTP722PA-1Brain cellsBHP1
XSTP723PA-1Cancer cellsGE11
XSTP724PA-1Breast cancer cellsHER2
XSTP725PA-1Antigen-presenting cellsCD40L/CD154
XSTP726PA-1Immune cellsIL-2

How It Works

How It Works

Engineering exosomes with specific surface proteins

XStamp is based upon a C-terminal fusion of the C1C2 domain from the protein MFG-E8, which is targeted to the exosome surface. Protein sequences that are fused to the XStamp tag will efficiently display the protein ligand fusion on the surfaces of secreted exosomes.

Supporting Data

Supporting Data

See XStamp targeting in action

 
XStamp-NCAM and XStamp-BHP1 both target exosomes to neurons.

Figure 1. Both XStamp-NCAM and XStamp-BHP1 can target exosomes to neurons. METHODS: The XStamp-NCAM Lentivector (Cat.# XSTP721PA-1) and the XStamp-BHP1 Lentivector (Cat.# XSTP722PA-1), were transfected separately into mouse MSCs along with SBI’s XPack-GFP construct (Cat.# XPAK530PA-1), which packages GFP into the interior of exosomes to enable fluorescent tracking. Exosomes were isolated after 48 hours, and added to Neuro2a neuroblastoma cells in culture. The neurons were monitored for GFP delivery using phase and fluorescence microscopy after a 24-hour incubation.

FAQs

Resources

Citations

XStamp™-BHP1 Lentivector for Targeting Exosomes to Brain Cells $787.00

Products

Catalog Number Description Size Price Quantity Add to Cart
XSTP722PA-1 XStamp BHP1 brain targeting lentivector - uses BHP1 to target exosomes to the brain 10 µg $787
- +

Overview

Overview

Putting exosomes to work: Target exosomes to brain cells Use exosomes to deliver protein, RNA, DNA, or small molecule cargo to brain cells with XStamp-BHP1. The patented XStamp technology is an optimized, exosome surface display system that efficiently places protein sequences on exosomal surfaces. The XStamp-BHP1 Lentivector fuses the BHP1 peptide to the XStamp tag, ensuring that exosomes display BHP1 on their outer surface. Exosomes made from cells transfected or transduced with the XStamp-BHP1 Lentivector will now be targeted to brain cells.
  • Display BHP1 sequences on exosome surfaces
  • Target brain cells
  • Create stable XStamp-BHP1 cell lines
XStamp-BHP1 places BHP1 on the surface of your exosomes, targeting them to brain cells SBI also offers a selection of pre-built targeting XStamp Lentivectors, as well as a clone-your-own XStamp Cloning and Expression Vector:
Cat.#SpecificityTargeting Ligand Used
XSTP710PA-1Clone-your-ownYou decide
XSTP720PA-1GI tract cellsMotilin
XSTP721PA-1Neural cellsNCAM
XSTP722PA-1Brain cellsBHP1
XSTP723PA-1Cancer cellsGE11
XSTP724PA-1Breast cancer cellsHER2
XSTP725PA-1Antigen-presenting cellsCD40L/CD154
XSTP726PA-1Immune cellsIL-2

How It Works

How It Works

Engineering exosomes with specific surface proteins

XStamp is based upon a C-terminal fusion of the C1C2 domain from the protein MFG-E8, which is targeted to the exosome surface. Protein sequences that are fused to the XStamp tag will efficiently display the protein ligand fusion on the surfaces of secreted exosomes.

Supporting Data

Supporting Data

See XStamp targeting in action

 
XStamp-NCAM and XStamp-BHP1 both target exosomes to neurons.

Figure 1. Both XStamp-NCAM and XStamp-BHP1 can target exosomes to neurons. METHODS: The XStamp-NCAM Lentivector (Cat.# XSTP721PA-1) and the XStamp-BHP1 Lentivector (Cat.# XSTP722PA-1), were transfected separately into mouse MSCs along with SBI’s XPack-GFP construct (Cat.# XPAK530PA-1), which packages GFP into the interior of exosomes to enable fluorescent tracking. Exosomes were isolated after 48 hours, and added to Neuro2a neuroblastoma cells in culture. The neurons were monitored for GFP delivery using phase and fluorescence microscopy after a 24-hour incubation.

FAQs

Citations