XPack CMV-XP-Luciferase-EF1α-Puro Expression Lentivector
- Powerful—enables a wealth of applications, including
- Exosome labeling
- Exosome cargo tracking
- Exosomes as cargo delivery vehicles
- Exosomes as biotherapeutics
- Exosome-generating stable cell lines with protein cargo-of-interest
- Optimized—SBI developed the XPack peptide tag for robust exosome targeting
- Reliable—based on SBI’s highly-cited, well-proven lentivector technology
- Versatile
- Two promoter options for expression in a wide range of cells
- Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| XPAK532CL-1 | XPack CMV-XP-Luciferase-EF1α-PuroStable HEK293 Producer Cell Line | 2 x 10^6 Cells | $1364 |
|
||||
| XPAK532PA-1 | XPack CMV-XP-Luciferase-EF1α-Puro Expression Lentivector | 10 µg | $787 |
|
||||
| XPAK532VA-1 | XPack CMV-XP-Luciferase-EF1α-Puro Expression Lentivirus | >1 x 10^6 IFUs | $787 |
|
||||
Overview
Overview
Label exosomes with luciferase using XPack™
Easily create stable cell lines that produce luciferase-labeled exosomes with the XPack CMV-XP-Luciferase-EF1α-Puro Expression Lentivector. Take advantage of SBI’s well-proven lentivector technology to express luciferase from the strong CMV promoter (also available with an MSCV promoter for expression in hematopoietic and stem cells) and the optimized XPack exosome targeting tag to package luciferase into exosomes.
The XPack System at a glance:
- Powerful—enables a wealth of applications, including
- Exosome labeling
- Exosome cargo tracking
- Exosomes as cargo delivery vehicles
- Exosomes as biotherapeutics
- Exosome-generating stable cell lines with protein cargo-of-interest
- Optimized—SBI developed the XPack peptide tag for robust exosome targeting
- Reliable—based on SBI’s highly-cited, well-proven lentivector technology
- Versatile
- Two promoter options for expression in a wide range of cells
- Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors
| Reporter | CMV Promoter (For most cell lines) | MSCV Promoter (For hematopoietic and stem cell lines) | ||
|---|---|---|---|---|
Customize with our cloning vector |
||||
| XPAK510PA-1 | XPack CMV-XP-MCS-EF1α-Puro | XPAK710PA-1 | XPack MSCV-XP-MCS-EF1α-Puro | |
Label exosomes with pre-built reporters |
||||
| GFP | XPAK530CL-1 XPAK530PA-1 XPAK530VA-1 | XPack CMV-XP-GFP-EF1α-Puro | XPAK730PA-1 XPAK730VA-1 | XPack MSCV-XP-GFP-EF1α-Puro |
| RFP | XPAK531PA-1 | XPack CMV-XP-RFP-EF1α-Puro | ||
| Luciferase | XPAK532CL-1 XPAK532PA-1 XPAK532VA-1 | XPack CMV-XP-Luciferase-EF1α-Puro | XPAK732PA-1 XPAK732VA-1 | XPack MSCV-XP-Luciferase-EF1α-Puro |
References
How It Works
How It Works
Optimized for success
With XPack, SBI has developed an optimized N-terminal peptide sequence that targets the peptide—and any protein fused to it—to the inner surface of the exosome membrane. Our pre-built XPack-reporter constructs come in GFP, RFP, and luciferase options to cover a wide range of labeling needs.
Supporting Data
Supporting Data
XPack efficiently packages luciferase into exosomes which can be used to deliver GFP to target cells
Part 1: Packaging luciferase into exosomes with XPack
The XPack-Luciferase fusion protein is efficiently packaged as exosome cargo and delivered to target cells. HEK-293 cells were transfected with a plasmid encoding an N-terminal XPack-Luciferase fusion protein (Cat.# XPAK532PA-1). Forty-eight hours later, exosomes were isolated using ExoQuick-TC® and luciferase levels quantified and compared to exosomes isolated from untransfected cells (Figure 1). Only XPack-Luciferase exosomes show robust luminescence indicating efficient packaging of luciferase into exosomes.
Figure 1. XPack efficiently packages luciferase into exosomes.
Part 2: XPack-Luciferase Exosome Delivery to Target Cells
The XPack-Luciferase exosomes isolated as described above were then added to recipient HEK-293 cells to test for luciferase delivery. After 24-hours, robust luminescence from cells exposed to XPack-Luciferase exosomes shows delivery of the luciferase cargo to the target cells (Figure 2). In contrast, untreated cells or cells treated with exosomes from untransfected cells show no luminescence.
Figure 2. XPack-Luciferase exosomes deliver luciferase to target cells.
FAQs
Resources
Citations
Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| XPAK532CL-1 | XPack CMV-XP-Luciferase-EF1α-PuroStable HEK293 Producer Cell Line | 2 x 10^6 Cells | $1364 |
|
||||
| XPAK532PA-1 | XPack CMV-XP-Luciferase-EF1α-Puro Expression Lentivector | 10 µg | $787 |
|
||||
| XPAK532VA-1 | XPack CMV-XP-Luciferase-EF1α-Puro Expression Lentivirus | >1 x 10^6 IFUs | $787 |
|
||||
Overview
Overview
Label exosomes with luciferase using XPack™
Easily create stable cell lines that produce luciferase-labeled exosomes with the XPack CMV-XP-Luciferase-EF1α-Puro Expression Lentivector. Take advantage of SBI’s well-proven lentivector technology to express luciferase from the strong CMV promoter (also available with an MSCV promoter for expression in hematopoietic and stem cells) and the optimized XPack exosome targeting tag to package luciferase into exosomes.
The XPack System at a glance:
- Powerful—enables a wealth of applications, including
- Exosome labeling
- Exosome cargo tracking
- Exosomes as cargo delivery vehicles
- Exosomes as biotherapeutics
- Exosome-generating stable cell lines with protein cargo-of-interest
- Optimized—SBI developed the XPack peptide tag for robust exosome targeting
- Reliable—based on SBI’s highly-cited, well-proven lentivector technology
- Versatile
- Two promoter options for expression in a wide range of cells
- Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors
| Reporter | CMV Promoter (For most cell lines) | MSCV Promoter (For hematopoietic and stem cell lines) | ||
|---|---|---|---|---|
Customize with our cloning vector |
||||
| XPAK510PA-1 | XPack CMV-XP-MCS-EF1α-Puro | XPAK710PA-1 | XPack MSCV-XP-MCS-EF1α-Puro | |
Label exosomes with pre-built reporters |
||||
| GFP | XPAK530CL-1 XPAK530PA-1 XPAK530VA-1 | XPack CMV-XP-GFP-EF1α-Puro | XPAK730PA-1 XPAK730VA-1 | XPack MSCV-XP-GFP-EF1α-Puro |
| RFP | XPAK531PA-1 | XPack CMV-XP-RFP-EF1α-Puro | ||
| Luciferase | XPAK532CL-1 XPAK532PA-1 XPAK532VA-1 | XPack CMV-XP-Luciferase-EF1α-Puro | XPAK732PA-1 XPAK732VA-1 | XPack MSCV-XP-Luciferase-EF1α-Puro |
References
How It Works
How It Works
Optimized for success
With XPack, SBI has developed an optimized N-terminal peptide sequence that targets the peptide—and any protein fused to it—to the inner surface of the exosome membrane. Our pre-built XPack-reporter constructs come in GFP, RFP, and luciferase options to cover a wide range of labeling needs.
Supporting Data
Supporting Data
XPack efficiently packages luciferase into exosomes which can be used to deliver GFP to target cells
Part 1: Packaging luciferase into exosomes with XPack
The XPack-Luciferase fusion protein is efficiently packaged as exosome cargo and delivered to target cells. HEK-293 cells were transfected with a plasmid encoding an N-terminal XPack-Luciferase fusion protein (Cat.# XPAK532PA-1). Forty-eight hours later, exosomes were isolated using ExoQuick-TC® and luciferase levels quantified and compared to exosomes isolated from untransfected cells (Figure 1). Only XPack-Luciferase exosomes show robust luminescence indicating efficient packaging of luciferase into exosomes.
Figure 1. XPack efficiently packages luciferase into exosomes.
Part 2: XPack-Luciferase Exosome Delivery to Target Cells
The XPack-Luciferase exosomes isolated as described above were then added to recipient HEK-293 cells to test for luciferase delivery. After 24-hours, robust luminescence from cells exposed to XPack-Luciferase exosomes shows delivery of the luciferase cargo to the target cells (Figure 2). In contrast, untreated cells or cells treated with exosomes from untransfected cells show no luminescence.
Figure 2. XPack-Luciferase exosomes deliver luciferase to target cells.