XPack MSCV-XP-Luciferase-EF1α-Puro Expression Lentivector

Label exosomes with luciferase and track uptake into target cells with XPack MSCV-XP-Luciferase-EF1α-Puro Expression Lentivector.
  • Powerful—enables a wealth of applications, including
    • Exosome labeling
    • Exosome cargo tracking
    • Exosomes as cargo delivery vehicles
    • Exosomes as biotherapeutics
    • Exosome-generating stable cell lines with protein cargo-of-interest
  • Optimized—SBI developed the XPack peptide tag for robust exosome targeting
  • Reliable—based on SBI’s highly-cited, well-proven lentivector technology
  • Versatile
    • Two promoter options for expression in a wide range of cells
    • Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors

Products

Catalog Number Description Size Price Quantity Add to Cart
XPAK732PA-1 XPack MSCV-XP-Luciferase-EF1α-Puro Expression Lentivector 10 µg $787
- +
XPAK732VA-1 XPack MSCV-XP-Luciferase-EF1α-Puro Expression Lentivirus >1 x 10^6 IFUs $787
- +

Overview

Overview

Label exosomes with luciferase using XPack™

Easily create stable cell lines that produce luciferase-labeled exosomes with the XPack MSCV-XP-Luciferase-EF1α-Puro Expression Lentivector. Take advantage of SBI’s well-proven lentivector technology to express luciferase from the strong MSCV promoter (also available with a CMV promoter for expression in hematopoietic and stem cells) and the optimized XPack exosome targeting tag to package luciferase into exosomes.

The XPack System at a glance:
  • Powerful—enables a wealth of applications, including
    • Exosome labeling
    • Exosome cargo tracking
    • Exosomes as cargo delivery vehicles
    • Exosomes as biotherapeutics
    • Exosome-generating stable cell lines with protein cargo-of-interest
  • Optimized—SBI developed the XPack peptide tag for robust exosome targeting
  • Reliable—based on SBI’s highly-cited, well-proven lentivector technology
  • Versatile
    • Two promoter options for expression in a wide range of cells
    • Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors
Choose the XPack Lentivector that’s right for you
 ReporterCMV Promoter (For most cell lines)MSCV Promoter (For hematopoietic and stem cell lines)
Customize with our cloning vector
XPAK510PA-1XPack CMV-XP-MCS-EF1α-PuroXPAK710PA-1 XPack MSCV-XP-MCS-EF1α-Puro
Label exosomes with pre-built reporters
GFPXPAK530CL-1

XPAK530PA-1

XPAK530VA-1
XPack CMV-XP-GFP-EF1α-PuroXPAK730PA-1

XPAK730VA-1
XPack MSCV-XP-GFP-EF1α-Puro
RFPXPAK531PA-1XPack CMV-XP-RFP-EF1α-Puro
LuciferaseXPAK532CL-1

XPAK532PA-1

XPAK532VA-1
XPack CMV-XP-Luciferase-EF1α-PuroXPAK732PA-1

XPAK732VA-1
XPack MSCV-XP-Luciferase-EF1α-Puro

References

How It Works

How It Works

Optimized for success

With XPack, SBI has developed an optimized N-terminal peptide sequence that targets the peptide—and any protein fused to it—to the inner surface of the exosome membrane. Our pre-built XPack-reporter constructs come in GFP, RFP, and luciferase options to cover a wide range of labeling needs.

Supporting Data

Supporting Data

XPack efficiently packages luciferase into exosomes which can be used to deliver GFP to target cells

Part 1: Packaging luciferase into exosomes with XPack

The XPack-Luciferase fusion protein is efficiently packaged as exosome cargo and delivered to target cells. HEK-293 cells were transfected with a plasmid encoding an N-terminal XPack-Luciferase fusion protein (Cat.# XPAK532PA-1). Forty-eight hours later, exosomes were isolated using ExoQuick-TC® and luciferase levels quantified and compared to exosomes isolated from untransfected cells (Figure 1). Only XPack-Luciferase exosomes show robust luminescence indicating efficient packaging of luciferase into exosomes.

XPack efficiently packages luciferase into exosomes

Figure 1. XPack efficiently packages luciferase into exosomes.

Part 2: XPack-Luciferase Exosome Delivery to Target Cells

The XPack-Luciferase exosomes isolated as described above were then added to recipient HEK-293 cells to test for luciferase delivery. After 24-hours, robust luminescence from cells exposed to XPack-Luciferase exosomes shows delivery of the luciferase cargo to the target cells (Figure 2). In contrast, untreated cells or cells treated with exosomes from untransfected cells show no luminescence.XPack-Luciferase exosomes deliver luciferase to target cells

Figure 2. XPack-Luciferase exosomes deliver luciferase to target cells.

FAQs

Resources

Citations

Products

Catalog Number Description Size Price Quantity Add to Cart
XPAK732PA-1 XPack MSCV-XP-Luciferase-EF1α-Puro Expression Lentivector 10 µg $787
- +
XPAK732VA-1 XPack MSCV-XP-Luciferase-EF1α-Puro Expression Lentivirus >1 x 10^6 IFUs $787
- +

Overview

Overview

Label exosomes with luciferase using XPack™

Easily create stable cell lines that produce luciferase-labeled exosomes with the XPack MSCV-XP-Luciferase-EF1α-Puro Expression Lentivector. Take advantage of SBI’s well-proven lentivector technology to express luciferase from the strong MSCV promoter (also available with a CMV promoter for expression in hematopoietic and stem cells) and the optimized XPack exosome targeting tag to package luciferase into exosomes.

The XPack System at a glance:
  • Powerful—enables a wealth of applications, including
    • Exosome labeling
    • Exosome cargo tracking
    • Exosomes as cargo delivery vehicles
    • Exosomes as biotherapeutics
    • Exosome-generating stable cell lines with protein cargo-of-interest
  • Optimized—SBI developed the XPack peptide tag for robust exosome targeting
  • Reliable—based on SBI’s highly-cited, well-proven lentivector technology
  • Versatile
    • Two promoter options for expression in a wide range of cells
    • Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors
Choose the XPack Lentivector that’s right for you
 ReporterCMV Promoter (For most cell lines)MSCV Promoter (For hematopoietic and stem cell lines)
Customize with our cloning vector
XPAK510PA-1XPack CMV-XP-MCS-EF1α-PuroXPAK710PA-1 XPack MSCV-XP-MCS-EF1α-Puro
Label exosomes with pre-built reporters
GFPXPAK530CL-1

XPAK530PA-1

XPAK530VA-1
XPack CMV-XP-GFP-EF1α-PuroXPAK730PA-1

XPAK730VA-1
XPack MSCV-XP-GFP-EF1α-Puro
RFPXPAK531PA-1XPack CMV-XP-RFP-EF1α-Puro
LuciferaseXPAK532CL-1

XPAK532PA-1

XPAK532VA-1
XPack CMV-XP-Luciferase-EF1α-PuroXPAK732PA-1

XPAK732VA-1
XPack MSCV-XP-Luciferase-EF1α-Puro

References

How It Works

How It Works

Optimized for success

With XPack, SBI has developed an optimized N-terminal peptide sequence that targets the peptide—and any protein fused to it—to the inner surface of the exosome membrane. Our pre-built XPack-reporter constructs come in GFP, RFP, and luciferase options to cover a wide range of labeling needs.

Supporting Data

Supporting Data

XPack efficiently packages luciferase into exosomes which can be used to deliver GFP to target cells

Part 1: Packaging luciferase into exosomes with XPack

The XPack-Luciferase fusion protein is efficiently packaged as exosome cargo and delivered to target cells. HEK-293 cells were transfected with a plasmid encoding an N-terminal XPack-Luciferase fusion protein (Cat.# XPAK532PA-1). Forty-eight hours later, exosomes were isolated using ExoQuick-TC® and luciferase levels quantified and compared to exosomes isolated from untransfected cells (Figure 1). Only XPack-Luciferase exosomes show robust luminescence indicating efficient packaging of luciferase into exosomes.

XPack efficiently packages luciferase into exosomes

Figure 1. XPack efficiently packages luciferase into exosomes.

Part 2: XPack-Luciferase Exosome Delivery to Target Cells

The XPack-Luciferase exosomes isolated as described above were then added to recipient HEK-293 cells to test for luciferase delivery. After 24-hours, robust luminescence from cells exposed to XPack-Luciferase exosomes shows delivery of the luciferase cargo to the target cells (Figure 2). In contrast, untreated cells or cells treated with exosomes from untransfected cells show no luminescence.XPack-Luciferase exosomes deliver luciferase to target cells

Figure 2. XPack-Luciferase exosomes deliver luciferase to target cells.

FAQs

Citations