XStamp™ Cloning and Expression Lentivector
- Display proteins on exosome surfaces
- Coat exosomes with targeting ligands
- Target specific cellular destinations
- Create stable XStamp cell lines
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
XSTP710PA-1 | XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1α-Puro | 10 µg | $757 |
|
Overview
Overview
Putting exosomes to work: Target exosomes to specific tissues
Use exosomes to deliver protein, RNA, DNA, or small molecule cargo to specific tissues with XStamp. The patented XStamp technology is an optimized, exosome surface display system that efficiently places protein sequences on exosomal surfaces. When a tissue-specific protein ligand is used, the engineered exosomes become targeted to the specified tissue, enabling directed cargo delivery.
- Display proteins on exosome surfaces
- Coat exosomes with targeting ligands
- Target specific cellular destinations
- Create stable XStamp cell lines
Cat.# | Specificity | Targeting Ligand Used |
---|---|---|
XSTP710PA-1 | Clone-your-own | You decide |
XSTP720PA-1 | GI tract cells | Motilin |
XSTP721PA-1 | Neural cells | NCAM |
XSTP722PA-1 | Brain cells | BHP1 |
XSTP723PA-1 | Cancer cells | GE11 |
XSTP724PA-1 | Breast cancer cells | HER2 |
XSTP725PA-1 | Antigen-presenting cells | CD40L/CD154 |
XSTP726PA-1 | Immune cells | IL-2 |
References
How It Works
How It Works
Engineering exosomes with specific surface proteins
XStamp is based upon a C-terminal fusion of the C1C2 domain from the protein MFG-E8, which is targeted to the exosome surface. Protein sequences that are fused to the XStamp tag will efficiently display the protein ligand fusion on the surfaces of secreted exosomes.
To target your protein ligand to the exosome surface, simply clone the gene encoding your protein ligand-of-interest into the MCS site of the XStamp Cloning and Expression Vector. Your ligand will have a 5’ secretion signal sequence and a C-term XStamp. The XStamp Lentivector also features a downstream EF1-Puromycin cassette for positive selection and, when packaged into virus, the XStamp Lentivector can be used to create stable cell lines that secrete your engineered exosomes.
Supporting Data
FAQs
Resources
Citations
Related Products
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
XSTP710PA-1 | XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1α-Puro | 10 µg | $757 |
|
Overview
Overview
Putting exosomes to work: Target exosomes to specific tissues
Use exosomes to deliver protein, RNA, DNA, or small molecule cargo to specific tissues with XStamp. The patented XStamp technology is an optimized, exosome surface display system that efficiently places protein sequences on exosomal surfaces. When a tissue-specific protein ligand is used, the engineered exosomes become targeted to the specified tissue, enabling directed cargo delivery.
- Display proteins on exosome surfaces
- Coat exosomes with targeting ligands
- Target specific cellular destinations
- Create stable XStamp cell lines
Cat.# | Specificity | Targeting Ligand Used |
---|---|---|
XSTP710PA-1 | Clone-your-own | You decide |
XSTP720PA-1 | GI tract cells | Motilin |
XSTP721PA-1 | Neural cells | NCAM |
XSTP722PA-1 | Brain cells | BHP1 |
XSTP723PA-1 | Cancer cells | GE11 |
XSTP724PA-1 | Breast cancer cells | HER2 |
XSTP725PA-1 | Antigen-presenting cells | CD40L/CD154 |
XSTP726PA-1 | Immune cells | IL-2 |
References
How It Works
How It Works
Engineering exosomes with specific surface proteins
XStamp is based upon a C-terminal fusion of the C1C2 domain from the protein MFG-E8, which is targeted to the exosome surface. Protein sequences that are fused to the XStamp tag will efficiently display the protein ligand fusion on the surfaces of secreted exosomes.
To target your protein ligand to the exosome surface, simply clone the gene encoding your protein ligand-of-interest into the MCS site of the XStamp Cloning and Expression Vector. Your ligand will have a 5’ secretion signal sequence and a C-term XStamp. The XStamp Lentivector also features a downstream EF1-Puromycin cassette for positive selection and, when packaged into virus, the XStamp Lentivector can be used to create stable cell lines that secrete your engineered exosomes.