EXOCET Exosome Quantitation Kit
- Fast—complete in 20 minutes
- Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes
- Quantitative—included calibration standards enable calculation of the number of exosome particles
- Flexible—works with all mammalian species tested (human, mouse, rat)
- Fully compatible—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXOCET96A-1 | EXOCET Exosome Quantitation Kit | 96 Reactions | $479 |
|
Overview
Overview
Move quickly with SBI’s fastest exosome quantitation method
With the EXOCET Exosome Quantitation Kit, you can find out just how many exosomes you’ve isolated in as little as 20 minutes. This antibody-free, colorimetric assay (OD405) measures the activity of Acetyl-CoA Acetylcholinesterase (AChE), an enzyme shown to be highly enriched in exosomes1, 2 (using mass spectrometry and Western blotting, SBI has verified the presence of AChE in exosomes from serum, stem cells, cancer cell lines, and MSCs—contact tech@systembio.com to learn more).
EXOCET is a highly quantitative assay and the standards included with each kit have been calibrated using NanoSight analysis, enabling calculation of the number of exosomes in your samples. The EXOCET Kit contains all of the necessary assay reagents and an optical 96-well plate to perform 96 reactions.
- Fast—complete in 20 minutes
- Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes
- Quantitative—included calibration standards enable calculation of the number of exosome particles
- Flexible—works with all mammalian species tested (human, mouse, rat)
- Fully compatible—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography
ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
---|---|---|---|
Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
Detection method | Antibody | Enzymatic | Enzymatic |
Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 | EXOCET | FluoroCet |
- Savina A, et al. The exosome pathway in K562 cells is regulated by Rab11. J Cell Sci. 2002 Jun 15. 115(Pt 12):2505-15. PMID: 12045221.
- Gupta S, and Knowlton AA. HSP60 trafficking in adult cardiac myocytes: role of the exosomal pathway. Am J Physiol Heart Circ Physiol. 2007 Jun. 292(6):H3052-6. PMID: 17307989.
How It Works
How It Works
Exosome quantitation with EXOCET is quick and easy
- Isolate exosomes (ExoQuick, ExoQuick-TC, ExoQuick PLUS, and ExoQuick-TC PLUS are all excellent methods)
- Lyse exosomes with the included Exosome Lysis Buffer
- Measure AChE activity—add the included buffer and incubate for 10–20 minutes. Readout is at 405 nm
Supporting Data
FAQs
Resources
Related Products
Citations
-
Irep, N, et al. (2024) Exosome inhibition improves response to first-line therapy in small cell lung cancer. Journal of cellular and molecular medicine. 2024; 28(4):e18138. PM ID: 38353469
-
Karacam, B, et al. (2024) Role of cell-free DNA and extracellular vesicles for diagnosis and surveillance in patients with glioma. The Journal of Liquid Biopsy. 2024; 4:100142. Link: The Journal of Liquid Biopsy
-
Ock, J, et al. (2023) Heme-binding protein 1 delivered via pericyte-derived extracellular vesicles improves neurovascular regeneration in a mouse model of cavernous nerve injury. International Journal of Biological Sciences. 2023; 19(9):2663-2677. Link: International Journal of Biological Sciences
-
Yang, Z, et al. (2023) Human umbilical cord mesenchymal stem cell-derived extracellular vesicles loaded with TFCP2 activate Wnt/β-catenin signaling to alleviate preeclampsia. International Immunopharmacology. 2023; 115:109732. Link: International Immunopharmacology
-
Graça, AL, et al. (2023) Platelet-Derived Extracellular Vesicles Promote Tenogenic Differentiation of Stem Cells on Bioengineered Living Fibers. International journal of molecular sciences. 2023; 24(4). PM ID: 36834925
-
Wang, X, et al. (2023) RIP1 Mediates Manzamine-A-Induced Secretory Autophagy in Breast Cancer. Marine Drugs. 2023; 21(3):151. Link: Marine Drugs
-
Kawanishi, N, Tominaga, T & Suzuki, K. (2023) Electrical pulse stimulation-induced muscle contraction alters the microRNA and mRNA profiles of circulating extracellular vesicles in mice. American journal of physiology. Regulatory, integrative and comparative physiology. 2023;. PM ID: 37092746
-
Rodríguez‐Comas, J, et al. (2023) Immunoaffinity‐Based Microfluidic Platform for Exosomal MicroRNA Isolation from Obese and Lean Mouse Plasma. Advanced Materials Technologies. 2023;. Link: Advanced Materials Technologies
-
Arcuri, S, et al. (2023) 3D ECM-Based Scaffolds Boost Young Cell Secretome-Derived EV Rejuvenating Effects in Senescent Cells. International journal of molecular sciences. 2023; 24(9). PM ID: 37175996
-
WINSTON, T. (2023) Targeted mesenchymal stem cell differentiation from induced pluripotent stem cells for therapeutic application. Thesis. 2023;. Link: Thesis
-
Pedrosa, M, et al. (2023) Extracellular Vesicles and Their Renin-Angiotensin Cargo as a Link between Metabolic Syndrome and Parkinson’s Disease. Antioxidants. 2023; 12(12):2045. Link: Antioxidants
-
Sun, T, et al. (2023) Novel 3D-printing bilayer GelMA-based hydrogel containing BP,β-TCP and exosomes for cartilage-bone integrated repair. Biofabrication. 2023; 16(1). PM ID: 37857284
-
Jung, YK & Son, MH. (2023) Polydiacetylene-based aptasensors for rapid and specific colorimetric detection of malignant exosomes. Talanta. 2023; 268(Pt 1):125342. PM ID: 37918246
-
Toledo-Guardiola, SM, et al. (2023) Different seminal ejaculated fractions in artificial insemination condition the protein cargo of oviductal and uterine extracellular vesicles in pig. Frontiers in cell and developmental biology. 2023; 11:1231755. PM ID: 37868907
-
Ilaltdinov, AW. (2022) Exosome-and MicroRNA-Based Therapeutic Approach for Tendinopathy. Thesis. 2022;. Link: Thesis
-
Yin, GN, et al. (2022) Pericyte‑derived extracellular vesicles‑mimetic nanovesicles improves peripheral nerve regeneration in mouse models of sciatic nerve transection. International journal of molecular medicine. 2022; 49(2). PM ID: 34935051
-
Durur, DY, et al. (2022) Alteration of miRNAs in Small Neuron-Derived Extracellular Vesicles of Alzheimer’s Disease Patients and the Effect of Extracellular Vesicles on Microglial Immune Responses. Journal of molecular neuroscience : MN. 2022;. PM ID: 35488079
-
Anita, L, et al. (2022) Pericyte-derived extracellular vesicle-mimetic nanovesicles ameliorate erectile dysfunction via lipocalin 2 in diabetic mice. International Journal of Biological Sciences. 2022; 18(9):3653-3667. Link: International Journal of Biological Sciences
-
Rudraprasad, D, et al. (2022) Characterization and proteome profiling of extracellular vesicles in a murine model of Staphylococcus aureus endophthalmitis. Microbes and infection. 2022;:105014. PM ID: 35609864
-
Heale, K. (2022) A Biophysical and Molecular Characterization of Human Embryonic Stem Cell-Derived Exosomes. Thesis. 2022;. Link: Thesis
- See More
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXOCET96A-1 | EXOCET Exosome Quantitation Kit | 96 Reactions | $479 |
|
Overview
Overview
Move quickly with SBI’s fastest exosome quantitation method
With the EXOCET Exosome Quantitation Kit, you can find out just how many exosomes you’ve isolated in as little as 20 minutes. This antibody-free, colorimetric assay (OD405) measures the activity of Acetyl-CoA Acetylcholinesterase (AChE), an enzyme shown to be highly enriched in exosomes1, 2 (using mass spectrometry and Western blotting, SBI has verified the presence of AChE in exosomes from serum, stem cells, cancer cell lines, and MSCs—contact tech@systembio.com to learn more).
EXOCET is a highly quantitative assay and the standards included with each kit have been calibrated using NanoSight analysis, enabling calculation of the number of exosomes in your samples. The EXOCET Kit contains all of the necessary assay reagents and an optical 96-well plate to perform 96 reactions.
- Fast—complete in 20 minutes
- Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes
- Quantitative—included calibration standards enable calculation of the number of exosome particles
- Flexible—works with all mammalian species tested (human, mouse, rat)
- Fully compatible—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography
ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
---|---|---|---|
Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
Detection method | Antibody | Enzymatic | Enzymatic |
Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 | EXOCET | FluoroCet |
- Savina A, et al. The exosome pathway in K562 cells is regulated by Rab11. J Cell Sci. 2002 Jun 15. 115(Pt 12):2505-15. PMID: 12045221.
- Gupta S, and Knowlton AA. HSP60 trafficking in adult cardiac myocytes: role of the exosomal pathway. Am J Physiol Heart Circ Physiol. 2007 Jun. 292(6):H3052-6. PMID: 17307989.
How It Works
How It Works
Exosome quantitation with EXOCET is quick and easy
- Isolate exosomes (ExoQuick, ExoQuick-TC, ExoQuick PLUS, and ExoQuick-TC PLUS are all excellent methods)
- Lyse exosomes with the included Exosome Lysis Buffer
- Measure AChE activity—add the included buffer and incubate for 10–20 minutes. Readout is at 405 nm
FAQs
Citations
-
Irep, N, et al. (2024) Exosome inhibition improves response to first-line therapy in small cell lung cancer. Journal of cellular and molecular medicine. 2024; 28(4):e18138. PM ID: 38353469
-
Karacam, B, et al. (2024) Role of cell-free DNA and extracellular vesicles for diagnosis and surveillance in patients with glioma. The Journal of Liquid Biopsy. 2024; 4:100142. Link: The Journal of Liquid Biopsy
-
Ock, J, et al. (2023) Heme-binding protein 1 delivered via pericyte-derived extracellular vesicles improves neurovascular regeneration in a mouse model of cavernous nerve injury. International Journal of Biological Sciences. 2023; 19(9):2663-2677. Link: International Journal of Biological Sciences
-
Yang, Z, et al. (2023) Human umbilical cord mesenchymal stem cell-derived extracellular vesicles loaded with TFCP2 activate Wnt/β-catenin signaling to alleviate preeclampsia. International Immunopharmacology. 2023; 115:109732. Link: International Immunopharmacology
-
Graça, AL, et al. (2023) Platelet-Derived Extracellular Vesicles Promote Tenogenic Differentiation of Stem Cells on Bioengineered Living Fibers. International journal of molecular sciences. 2023; 24(4). PM ID: 36834925
-
Wang, X, et al. (2023) RIP1 Mediates Manzamine-A-Induced Secretory Autophagy in Breast Cancer. Marine Drugs. 2023; 21(3):151. Link: Marine Drugs
-
Kawanishi, N, Tominaga, T & Suzuki, K. (2023) Electrical pulse stimulation-induced muscle contraction alters the microRNA and mRNA profiles of circulating extracellular vesicles in mice. American journal of physiology. Regulatory, integrative and comparative physiology. 2023;. PM ID: 37092746
-
Rodríguez‐Comas, J, et al. (2023) Immunoaffinity‐Based Microfluidic Platform for Exosomal MicroRNA Isolation from Obese and Lean Mouse Plasma. Advanced Materials Technologies. 2023;. Link: Advanced Materials Technologies
-
Arcuri, S, et al. (2023) 3D ECM-Based Scaffolds Boost Young Cell Secretome-Derived EV Rejuvenating Effects in Senescent Cells. International journal of molecular sciences. 2023; 24(9). PM ID: 37175996
-
WINSTON, T. (2023) Targeted mesenchymal stem cell differentiation from induced pluripotent stem cells for therapeutic application. Thesis. 2023;. Link: Thesis
-
Pedrosa, M, et al. (2023) Extracellular Vesicles and Their Renin-Angiotensin Cargo as a Link between Metabolic Syndrome and Parkinson’s Disease. Antioxidants. 2023; 12(12):2045. Link: Antioxidants
-
Sun, T, et al. (2023) Novel 3D-printing bilayer GelMA-based hydrogel containing BP,β-TCP and exosomes for cartilage-bone integrated repair. Biofabrication. 2023; 16(1). PM ID: 37857284
-
Jung, YK & Son, MH. (2023) Polydiacetylene-based aptasensors for rapid and specific colorimetric detection of malignant exosomes. Talanta. 2023; 268(Pt 1):125342. PM ID: 37918246
-
Toledo-Guardiola, SM, et al. (2023) Different seminal ejaculated fractions in artificial insemination condition the protein cargo of oviductal and uterine extracellular vesicles in pig. Frontiers in cell and developmental biology. 2023; 11:1231755. PM ID: 37868907
-
Ilaltdinov, AW. (2022) Exosome-and MicroRNA-Based Therapeutic Approach for Tendinopathy. Thesis. 2022;. Link: Thesis
-
Yin, GN, et al. (2022) Pericyte‑derived extracellular vesicles‑mimetic nanovesicles improves peripheral nerve regeneration in mouse models of sciatic nerve transection. International journal of molecular medicine. 2022; 49(2). PM ID: 34935051
-
Durur, DY, et al. (2022) Alteration of miRNAs in Small Neuron-Derived Extracellular Vesicles of Alzheimer’s Disease Patients and the Effect of Extracellular Vesicles on Microglial Immune Responses. Journal of molecular neuroscience : MN. 2022;. PM ID: 35488079
-
Anita, L, et al. (2022) Pericyte-derived extracellular vesicle-mimetic nanovesicles ameliorate erectile dysfunction via lipocalin 2 in diabetic mice. International Journal of Biological Sciences. 2022; 18(9):3653-3667. Link: International Journal of Biological Sciences
-
Rudraprasad, D, et al. (2022) Characterization and proteome profiling of extracellular vesicles in a murine model of Staphylococcus aureus endophthalmitis. Microbes and infection. 2022;:105014. PM ID: 35609864
-
Heale, K. (2022) A Biophysical and Molecular Characterization of Human Embryonic Stem Cell-Derived Exosomes. Thesis. 2022;. Link: Thesis
- See More