ExoELISA-ULTRA BamA Complete Kit
(For Gram-negative Bacterial OMV Detection)
The first and only kit for easy quantitation and characterization of bacterial outer membrane vesicles (OMVs) isolated from Gram-negative bacteria. Quickly quantify bacterial OMVs using BamA detection with this sensitive ELISA-based assay suitable for common OMV isolation methods.- Sensitive - detect as little as 1 µg protein equivalent
- Fast - complete in less than 4-hours; no more overnight incubation
- Flexible - compatible with common OMV isolation methods (e.g. ExoBacteria™ OMV Isolation Kit, ultracentrifugation) from bacterial culture media
- Quantitative - calibrated internal standards enable quantitation of OMVs carrying BamA
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXEL-ULTRA-BamA-1 | ExoELISA-ULTRA BamA Complete Kit (For Gram-neg. Bacterial OMV Detection) | 96 Reactions | $699 |
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Overview
Completing the collection of our popular ExoELISA-ULTRA Kits, the ExoELISA-ULTRA BamA Kit is brought to users as the first and only kit for easy quantitation and characterization of OMVs from Gram-negative bacteria, with supreme sensitivity of OMV detection - as low as 1 µg protein equivalent - and a total assay time of only 4 hours.
Bacterial outer membrane vesicles (OMVs) play crucial roles in pathogenesis and intercellular interactions, yet a universal set of OMV marker proteins remains elusive. BamA, an essential subunit of the β-barrel assembly machine (BAM), is a highly conserved outer membrane protein found in all Gram-negative bacterial species and consistently enriched in purified bacterial OMVs (1-4). To facilitate the detection of OMVs from Gram-negative bacteria, BamA was chosen as the OMV marker. A custom antibody targeting the conserved C-terminal peptide of BamA was developed to ensure reactivity across all Gram-negative bacterial species (5-6). The ExoELISA-ULTRA OMV detection assay employs an ultra-sensitive direct capture colorimetric ELISA method compatible with OMVs isolated by common methods. For quantifying BamA-carrying OMVs in your sample, the kit includes an internal standard calibrated to OMVs isolated with various methods, validated by nanoparticle tracking analysis (NTA).
One ExoELISA-ULTRA OMV Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.
- Sensitive - detect as little as 1 µg protein equivalent
- Fast - complete in less than 4-hours; no more overnight incubation
- Flexible - compatible with common OMV isolation methods (e.g. ExoBacteria™ OMV Isolation Kit, ultracentrifugation) from bacterial culture media
- Quantitative - calibrated internal standards enable quantitation of OMVs carrying BamA
References
- Doyle MT, Bernstein HD. BamA forms a translocation channel for polypeptide export across the bacterial outer membrane. Mol Cell. 2021 May 6; 81(9):2000-2012.e3. doi: 10.1016/j.molcel.2021.02.023.
- Li Y, et al. Identification of a Compound That Inhibits the Growth of Gram-Negative Bacteria by Blocking BamA-BamD Interaction. Front Microbiol. 2020 Jun 19; 11:1252. doi: 10.3389/fmicb.2020.01252.
- Voulhoux R, et al. Role of a highly conserved bacterial protein in outer membrane protein assembly. Science. 2003 Jan 10; 299(5604):262-5. doi: 10.1126/science.1078973.
- Hong J, Dauros-Singorenko P, Whitcombe A, Payne L, Blenkiron C, Phillips A, Swift S. Analysis of the Escherichia coli extracellular vesicle proteome identifies markers of purity and culture conditions. J Extracell Vesicles. 2019 Jun 24;8(1):1632099. doi: 10.1080/20013078.2019.1632099.
- Pavlova O, et al. Mechanistic link between β barrel assembly and the initiation of autotransporter secretion. Proc Natl Acad Sci U S A. 2013 Mar 5; 110(10):E938-47. doi: 10.1073/pnas.1219076110.
- Vieira de Araujo AE, et al. Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii. Microbes Infect. 2021 May-Jun; 23(4-5):104801. doi: 10.1016/j.micinf.2021.104801.
Associated Kits
| ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
|---|---|---|---|
| Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
| Detection method | Antibody | Enzymatic | Enzymatic |
| Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
| Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
| Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
| Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 ExoELISA-ULTRA GroEL | EXOCET | FluoroCet |
How It Works
Supporting Data
Figure 1. ExoELISA-ULTRA BamA standard curve shows robust linearity down to 3.61 x 109 OMVs. OD 450 nm values of OMVs isolated with common OMV isolation methods (Ultracentrifuge and ExoBacteria™ OMV Isolation Kit) fall well within the standard curve for the assay.
FAQs
Resources
Citations
Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXEL-ULTRA-BamA-1 | ExoELISA-ULTRA BamA Complete Kit (For Gram-neg. Bacterial OMV Detection) | 96 Reactions | $699 |
|
||||
Overview
Completing the collection of our popular ExoELISA-ULTRA Kits, the ExoELISA-ULTRA BamA Kit is brought to users as the first and only kit for easy quantitation and characterization of OMVs from Gram-negative bacteria, with supreme sensitivity of OMV detection - as low as 1 µg protein equivalent - and a total assay time of only 4 hours.
Bacterial outer membrane vesicles (OMVs) play crucial roles in pathogenesis and intercellular interactions, yet a universal set of OMV marker proteins remains elusive. BamA, an essential subunit of the β-barrel assembly machine (BAM), is a highly conserved outer membrane protein found in all Gram-negative bacterial species and consistently enriched in purified bacterial OMVs (1-4). To facilitate the detection of OMVs from Gram-negative bacteria, BamA was chosen as the OMV marker. A custom antibody targeting the conserved C-terminal peptide of BamA was developed to ensure reactivity across all Gram-negative bacterial species (5-6). The ExoELISA-ULTRA OMV detection assay employs an ultra-sensitive direct capture colorimetric ELISA method compatible with OMVs isolated by common methods. For quantifying BamA-carrying OMVs in your sample, the kit includes an internal standard calibrated to OMVs isolated with various methods, validated by nanoparticle tracking analysis (NTA).
One ExoELISA-ULTRA OMV Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.
- Sensitive - detect as little as 1 µg protein equivalent
- Fast - complete in less than 4-hours; no more overnight incubation
- Flexible - compatible with common OMV isolation methods (e.g. ExoBacteria™ OMV Isolation Kit, ultracentrifugation) from bacterial culture media
- Quantitative - calibrated internal standards enable quantitation of OMVs carrying BamA
References
- Doyle MT, Bernstein HD. BamA forms a translocation channel for polypeptide export across the bacterial outer membrane. Mol Cell. 2021 May 6; 81(9):2000-2012.e3. doi: 10.1016/j.molcel.2021.02.023.
- Li Y, et al. Identification of a Compound That Inhibits the Growth of Gram-Negative Bacteria by Blocking BamA-BamD Interaction. Front Microbiol. 2020 Jun 19; 11:1252. doi: 10.3389/fmicb.2020.01252.
- Voulhoux R, et al. Role of a highly conserved bacterial protein in outer membrane protein assembly. Science. 2003 Jan 10; 299(5604):262-5. doi: 10.1126/science.1078973.
- Hong J, Dauros-Singorenko P, Whitcombe A, Payne L, Blenkiron C, Phillips A, Swift S. Analysis of the Escherichia coli extracellular vesicle proteome identifies markers of purity and culture conditions. J Extracell Vesicles. 2019 Jun 24;8(1):1632099. doi: 10.1080/20013078.2019.1632099.
- Pavlova O, et al. Mechanistic link between β barrel assembly and the initiation of autotransporter secretion. Proc Natl Acad Sci U S A. 2013 Mar 5; 110(10):E938-47. doi: 10.1073/pnas.1219076110.
- Vieira de Araujo AE, et al. Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii. Microbes Infect. 2021 May-Jun; 23(4-5):104801. doi: 10.1016/j.micinf.2021.104801.
Associated Kits
| ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
|---|---|---|---|
| Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
| Detection method | Antibody | Enzymatic | Enzymatic |
| Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
| Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
| Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
| Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 ExoELISA-ULTRA GroEL | EXOCET | FluoroCet |
How It Works
Supporting Data
Figure 1. ExoELISA-ULTRA BamA standard curve shows robust linearity down to 3.61 x 109 OMVs. OD 450 nm values of OMVs isolated with common OMV isolation methods (Ultracentrifuge and ExoBacteria™ OMV Isolation Kit) fall well within the standard curve for the assay.