XStamp™-CD40L Lentivector for Targeting Exosomes to Antigen-presenting cells

Target exosomes to antigen-presenting cells with the pre-built XStamp-CD40L Lentivector Construct.

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XStamp CD40 receptor targeting lentivector – CD40L exosomes target cells with surface CD40 receptor

10 µg
$ 697
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Putting exosomes to work: Target exosomes to antigen-presenting cells

Use exosomes to deliver protein, RNA, DNA, or small molecule cargo to antigen-presenting cells with XStamp-CD40L. The patented XStamp technology is an optimized, exosome surface display system that efficiently places protein sequences on exosomal surfaces. The XStamp-CD40L Lentivector fuses CD40L sequences to the XStamp tag, ensuring that exosomes display the CD40L on their outer surface. Exosomes made from cells transfected or transduced with the XStamp-CD40L Lentivector will now be targeted to cells expressing CD40, primarily antigen-presenting cells.

  • Display CD40L sequences on exosome surfaces
  • Target antigen-presenting cells
  • Create stable XStamp-CD40L cell lines

XStamp-CD40L places CD40L sequences on the surface of your exosomes, targeting them to antigen-presenting cells

SBI also offers a selection of pre-built targeting XStamp Lentivectors, as well as a clone-your-own XStamp Cloning and Expression Vector:

Cat.# Specificity Targeting Ligand Used
XSTP710PA-1 Clone-your-own You decide
XSTP720PA-1 GI tract cells Motilin
XSTP721PA-1 Neural cells NCAM
XSTP722PA-1 Brain cells BHP1
XSTP723PA-1 Cancer cells GE11
XSTP724PA-1 Breast cancer cells HER2
XSTP725PA-1 Antigen-presenting cells CD40L/CD154
XSTP726PA-1 Immune cells IL-2

How It Works

Engineering exosomes with specific surface proteins

XStamp is based upon a C-terminal fusion of the C1C2 domain from the protein MFG-E8, which is targeted to the exosome surface. Protein sequences that are fused to the XStamp tag will efficiently display the protein ligand fusion on the surfaces of secreted exosomes.

Supporting Data

See XStamp targeting in action

XStamp-motilin targets exosomes to GI cells.

Figure 1. XStamp with motilin specifically targets exosomes to GI cells. Motilin is a 22-amino acid polypeptide hormone that binds to the motilin receptor, which is exclusively expressed in the intestine. XStamp with motilin can be used to target exosomes to GI cells. METHODS: The XStamp-Motilin construct (Cat.# XSTP720PA-1) was transfected into HEK293 cells and after 48 hours, the exosomes were isoalted using ExoQuick-TC. The next day, the XStamp-Motilin exosomes were Exo-Fected with a Texas-Red-labeled siRNA to monitor exosome docking and delivery. The transfected XStamp-Motilin exosomes were then added to MDA-MB-231 Breast Cancer Cells (motilin receptor negative) and to HT-29 Colon Cancer Cells (motilin receptor positive). The cells were imaged after 24 hours for uptake of the Texas-Red-labeled siRNA delivery from the XStamped exosomes. The HT-29 colon cancer cells that are motilin receptor positive took up the XStamp-Motilin exosomes at a much higher rate than the MDA-MB-231 Breast Cancer (motilin receptor negative) cells.