Move quickly with SBI’s fastest exosome quantitation method
With the EXOCET Exosome Quantitation Kit, you can find out just how many exosomes you’ve isolated in as little as 20 minutes. This antibody-free, colorimetric assay (OD405) measures the activity of Acetyl-CoA Acetylcholinesterase (AChE), an enzyme shown to be highly enriched in exosomes1, 2 (using mass spectrometry and Western blotting, SBI has verified the presence of AChE in exosomes from serum, stem cells, cancer cell lines, and MSCs—contact email@example.com to learn more).
EXOCET is a highly quantitative assay and the standards included with each kit have been calibrated using NanoSight analysis, enabling calculation of the number of exosomes in your samples. The EXOCET Kit contains all of the necessary assay reagents and an optical 96-well plate to perform 96 reactions.
- Fast—complete in 20 minutes
- Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes
- Quantitative—included calibration standards enable calculation of the number of exosome particles
- Flexible—works with all mammalian species tested (human, mouse, rat)
- Fully compatible—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography
Choose the exosome quantitation method that’s best for your studies
|Use||For fast and sensitive antibody-based quantitation of exosomes||For sensitive quantitation of exosomes when time and input sample are not limiting||For fast quantitation of extracellular vesicles with moderate sample input requirements||For the most sensitive quantitation of extracellular vesicles with very low sample input requirements|
|Quantitation chemistry||Enzymatic (HRP)||Enzymatic (HRP)||Colorimetric||Fluorescent|
|Total protocol time||4 hours (no overnight incubation)||24 hours||20 min||60 min|
|Input sample amount (protein equivalent)||1 – 200 µg||>500 µg||50 µg||<1 µg|
|Learn More||ExoELISA-ULTRA CD63||ExoELISA CD9
- Savina A, et al. The exosome pathway in K562 cells is regulated by Rab11. J Cell Sci. 2002 Jun 15. 115(Pt 12):2505-15. PMID: 12045221.
- Gupta S, and Knowlton AA. HSP60 trafficking in adult cardiac myocytes: role of the exosomal pathway. Am J Physiol Heart Circ Physiol. 2007 Jun. 292(6):H3052-6. PMID: 17307989.