EXOCET Standards

Standards for our fast and easy EXOCET assay

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EXOCET Standards

EXOCET-SD-1
$ 137
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Overview

Extra standards for EXOCET

While our fast and easy EXOCET Kit comes with standards, we also offer our EXOCET exosome standards as a stand-alone product for when you need more. The standards are calibrated using NanoSight analysis, enabling estimation of the number of exosomes in your samples.

The EXOCET Exosome Quantitation Kit is:

  • Fast—complete in 20 minutes
  • Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes
  • Quantitative—included calibration standards enable calculation of the number of exosome particles
  • Flexible—works with all mammalian species tested (human, mouse, rat)
  • Fully compatible—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography

Choose the exosome quantitation method that’s best for your studies

  ExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCET FluoroCet
Use For fast and sensitive antibody-based quantitation of exosomes For sensitive quantitation of exosomes when time and input sample are not limiting For fast quantitation of extracellular vesicles with moderate sample input requirements For the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection method Antibody Antibody Enzymatic Enzymatic
Quantitation chemistry Enzymatic (HRP) Enzymatic (HRP) Colorimetric Fluorescent
Total protocol time 4 hours (no overnight incubation) 24 hours 20 min 60 min
Input sample amount (protein equivalent) 1 – 200 µg >500 µg 50 µg <1 µg
Learn More ExoELISA-ULTRA CD63

ExoELISA-ULTRA CD81

ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCET FluoroCet

ExoELISA-ULTRA CD63 Detection ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCET FluoroCet
Use For fast and sensitive antibody-based quantitation of exosomes For sensitive quantitation of exosomes when time and input sample are not limiting For fast quantitation of extracellular vesicles with moderate sample input requirements For the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection method Antibody Antibody Enzymatic Enzymatic
Quantitation chemistry Enzymatic (HRP) Enzymatic (HRP) Colorimetric Fluorescent
Total protocol time 4 hours (no overnight incubation) 24 hours 20 min 60 min
Input sample amount (protein equivalent) 1 – 200 µg >500 µg 50 µg <1 µg

How It Works

Exosome quantitation with EXOCET is quick and easy

  1. Isolate exosomes (ExoQuick, ExoQuick-TC, ExoQuick PLUS, and ExoQuick-TC PLUS are all excellent methods)
  2. Lyse exosomes with the included Exosome Lysis Buffer
  3. Measure AChE activity—add the included buffer and incubate for 10–20 minutes. Readout is at 405 nm

Supporting Data

The fast, highly quantitative EXOCET exosome quantitation assay

Sample data and standard curve for EXOCET.

Exosome quantitation with EXOCET takes as little as 20 minutes and 50 µg (protein equivalent) of sample. Exosomes were isolated from either human serum (0.5 mL) or from MDA-MB-231 culture media (10 mL) using standard ExoQuick and ExoQuick-TC reagents. Exosome pellets were resuspended in PBS and total protein concentration measured using a BCA assay (~108 exosomes, 2 µg/µL). Exosomes were lysed using the EXOCET gentle lysis solution to preserve the enzymatic activity of the exosomal AChE enzyme. The standard curve was generated using known numbers of exosomes (as measured by NanoSight) and calibrated with a recombinant AChE enzyme standard solution provided in the kit.

Citations

  • Chen, CY, et al. (2019) Extracellular vesicles from human urine-derived stem cells prevent osteoporosis by transferring CTHRC1 and OPG. Bone Res. 2019 Jun 26; 7:18. PM ID: 31263627
  • Domenis, R, et al. (2019) Toll-like Receptor-4 Activation Boosts the Immunosuppressive Properties of Tumor Cells-derived Exosomes. Sci Rep. 2019 Jun 11; 9(1):8457. PM ID: 31186484
  • Kalimuthu, K, Kwon, WY & Park, KS. (2019) A simple approach for rapid and cost-effective quantification of extracellular vesicles using a fluorescence polarization technique. J Biol Eng. 2019 Apr 16; 13:31. PM ID: 31015861
  • Cornell, L, et al. (2019) MicroRNA-Mediated Suppression of the TGF-β Pathway Confers Transmissible and Reversible CDK4/6 Inhibitor Resistance. Cell Rep. 2019 Mar 5; 26(10):2667-2680.e7. PM ID: 30840889
  • Cornell, L, et al. (2019) MicroRNA-Mediated Suppression of the TGF-β Pathway Confers Transmissible and Reversible CDK4/6 Inhibitor Resistance. Cell Reports. 2019 Mar 1; 26(10):2667-2680.e7. Link: Cell Reports
  • Guay, C, et al. (2018) Lymphocyte-Derived Exosomal MicroRNAs Promote Pancreatic β Cell Death and May Contribute to Type 1 Diabetes Development. Cell Metab.. 2018 Oct 5;. PM ID: 30318337
  • Yang, R, et al. (2018) Tet1 and Tet2 maintain mesenchymal stem cell homeostasis via demethylation of the P2rX7 promoter. Nat Commun. 2018 Jun 1; 9(1):2143. PM ID: 29858571
  • Slowey, PD. (2017) Salivary Diagnostics Using Purified Nucleic Acids. Methods Mol. Biol.. 2017 Dec 7; 1537:3-15. PM ID: 27924585
  • Wu, X, et al. (2017) Tongxinluo Inhibits Renal Fibrosis in Diabetic Nephropathy: Involvement of the Suppression of Intercellular Transfer of TGF-ββ1-Containing Exosomes from GECs to GMCs Read More: http://www.worldscientific.com/doi/abs/10.1142/S0192415X17500586. The American Journal of Chinese Medicine. 2017 Jun 29;. Link: The American Journal of Chinese Medicine
  • Sharma, R, et al. (2017) Detection of phosphatidylserine-positive exosomes for the diagnosis of early-stage malignancies. Br. J. Cancer. 2017 Jun 22;. PM ID: 28641308
  • Domenis, R, et al. (2017) Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis. Mediators Inflamm.. 2017 Jun 21; 2017:4814987. PM ID: 28634420
  • Rody, WJ, et al. (2017) The use of cell culture platforms to identify novel markers of bone and dentin resorption. Orthod Craniofac Res. 2017 Jun 1;:89-94. PM ID: 28643914
  • Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology. Pediatr. Res.. 2017 Apr 5;. PM ID: 28157838
  • Jiang, X, et al. (2017) Exosomes from pediatric dilated cardiomyopathy patients modulate a pathological response in cardiomyocytes. Am. J. Physiol. Heart Circ. Physiol.. 2017 Apr 1; 312(4):H818-H826. PM ID: 28130338
  • Hurwitz, SN, et al. (2017) CD63 Regulates Epstein-Barr Virus LMP1 Exosomal Packaging, Enhancement of Vesicle Production, and Noncanonical NF-κB Signaling. J. Virol.. 2017 Mar 1; 91(5). PM ID: 27974566
  • Thomou, T, et al. (2017) Adipose-derived circulating miRNAs regulate gene expression in other tissues. Nature. 2017 Feb 23; 542(7642):450-455. PM ID: 28199304
  • Hovsepian, T, Saint-Auret, G & Lebot, C. (2017) A Skin Extracellular Signaling Pathway via Intercellular Exosomes Highlighted by Genetic and Epigenetic Analysis Applied to a Natural Ingredient. 29th IFSCC Congress. ;:23-28. Link: 29th IFSCC Congress
  • Lee, JY, et al. (2016) Circulating exosomes from patients with systemic lupus erythematosus induce an proinflammatory immune response. Arthritis Res. Ther.. 2016 Nov 16; 18(1):264. PM ID: 27852323
  • Rossaint, J, et al. (2016) Directed transport of neutrophil-derived extracellular vesicles enables platelet-mediated innate immune response. Nat Commun. 2016 Nov 15; 7:13464. PM ID: 27845343
  • Matsumoto, Y, et al. (2016) Quantification of plasma exosome is a potential prognostic marker for esophageal squamous cell carcinoma. Oncol. Rep.. 2016 Nov 1; 36(5):2535-2543. PM ID: 27599779