ExoELISA-ULTRA Complete Kit (CD63 Detection)

With a 4-hour total assay time, this sensitive ELISA-based assay speeds quantitation of exosomes from most biofluids

Description
Size
Catalog Number
Price
Quantity
Add to Cart

ExoELISA-ULTRA Complete Kit (CD63 detection)

96 Reactions
EXEL-ULTRA-CD63-1
$ 595
Contact Us Speak to a specialist
1-888-266-5066

Overview

Delivering ELISA-based exosome quantitation ULTRA fast

Improving on our popular ExoELISA Kits, the ExoELISA-ULTRA CD63 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.

Currently configured for detection of CD63, a widely recognized and popular exosomal marker1, ExoELISA-ULTRA CD63 is based on an ultra-sensitive, direct capture, colorimetric ELISA assay that is compatible with nearly all biofluids. The ExoELISA-ULTRA CD63 Kit comes with an internal standard calibrated to exosomes from a range of biofluids. Calibration is achieved by NanoSight analysis and enables quantitation of exosomes carrying CD63 in your target samples. One ExoELISA-ULTRA CD63 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.

  • Sensitive—detect as little as 1 µg protein equivalent
  • Fast—complete in less than 4-hours—no more overnight incubation
  • Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human, mouse, and rat
  • Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
  • Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications

Choose the exosome quantitation method that’s best for your studies

  ExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCET FluoroCet
Use For fast and sensitive antibody-based quantitation of exosomes For sensitive quantitation of exosomes when time and input sample are not limiting For fast quantitation of extracellular vesicles with moderate sample input requirements For the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection method Antibody Antibody Enzymatic Enzymatic
Quantitation chemistry Enzymatic (HRP) Enzymatic (HRP) Colorimetric Fluorescent
Total protocol time 4 hours (no overnight incubation) 24 hours 20 min 60 min
Input sample amount (protein equivalent) 1 – 200 µg >500 µg 50 µg <1 µg
Learn More ExoELISA-ULTRA CD63

ExoELISA-ULTRA CD81

ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCET FluoroCet


REFERENCES

  1. Kowal, J., et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016. February 23. 113(8): E968–E977. PMCID: PMC4776515.

Supporting Data

The standard curve for ExoELISA-ULTRA CD63 shows robust linearity down to ~1 x 109 exosomes.

The standard curve for ExoELISA-ULTRA CD63 provides robust linearity down to ~1 x 10^9 exosomes.


Citations

  • Kim, KS, et al. (2019) ELK3 expressed in lymphatic endothelial cells promotes breast cancer progression and metastasis through exosomal miRNAs. Sci Rep. 2019 Jun 10; 9(1):8418. PM ID: 31182803
  • Perut, F, et al. (2019) Extracellular Nanovesicles Secreted by Human Osteosarcoma Cells Promote Angiogenesis. Cancers (Basel). 2019 Jun 5; 11(6). PM ID: 31195680
  • Freitas, D, et al. (2019) Different isolation approaches lead to diverse glycosylated extracellular vesicle populations. J Extracell Vesicles. 2019 Jun 3; 8(1):1621131. PM ID: 31236201
  • Sung, PS, Huang, TF & Hsieh, SL. (2019) Extracellular vesicles from CLEC2-activated platelets enhance dengue virus-induced lethality via CLEC5A/TLR2. Nat Commun. 2019 Jun 3; 10(1):2402. PM ID: 31160588
  • Cui, Y, et al. (2019) EPC-derived exosomes promote osteoclastogenesis through LncRNA-MALAT1. J. Cell. Mol. Med.. 2019 Jun 1; 23(6):3843-3854. PM ID: 31025509
  • Guo, DF, et al. (2019) The BBSome in POMC and AgRP Neurons is Necessary for Body Weight Regulation and Sorting of Metabolic Receptors. Diabetes. 2019 May 24;. PM ID: 31127052
  • Maurel, M, et al. (2019) Control of anterior GRadient 2 (AGR2) dimerization links endoplasmic reticulum proteostasis to inflammation. EMBO Mol Med. 2019 Apr 30;. PM ID: 31040128
  • Parry, HA, et al. (2019) Bovine Milk Extracellular Vesicles (EVs) Modification Elicits Skeletal Muscle Growth in Rats. Front Physiol. 2019 Apr 16; 10:436. PM ID: 31040795
  • Chen, P, et al. (2019) Desktop-stereolithography 3D printing of a radially oriented extracellular matrix/mesenchymal stem cell exosome bioink for osteochondral defect regeneration. Theranostics. 2019 Apr 13; 9(9):2439-2459. PM ID: 31131046
  • Reiter, K, et al. (2019) Separation of virus-like particles and extracellular vesicles by flow-through and heparin affinity chromatography. J Chromatogr A. 2019 Mar 15; 1588:77-84. PM ID: 30616980
  • Tobón-Arroyave, SI, et al. (2019) Decreased salivary concentration of CD9 and CD81 exosome-related tetraspanins may be associated with the periodontal clinical status. J. Clin. Periodontol.. 2019 Mar 2;. PM ID: 30825338
  • Kim, JH, Lee, CH & Lee, SW. (2019) Exosomal Transmission of MicroRNA from HCV Replicating Cells Stimulates Transdifferentiation in Hepatic Stellate Cells. Mol Ther Nucleic Acids. 2019 Mar 1; 14:483-497. PM ID: 30753992
  • Capello, M, et al. (2019) Exosomes harbor B cell targets in pancreatic adenocarcinoma and exert decoy function against complement-mediated cytotoxicity. Nat Commun. 2019 Jan 16; 10(1):254. PM ID: 30651550
  • Zlotogorski-Hurvitz, A, et al. (2019) FTIR-based spectrum of salivary exosomes coupled with computational-aided discriminating analysis in the diagnosis of oral cancer. J. Cancer Res. Clin. Oncol.. 2019 Jan 3;. PM ID: 30603907
  • Baryeh, K. (2019) Development of Quantitative Lateral Flow Strip Biosensors for the Detection of Cancer Biomarkers. Thesis. ;. Link: Thesis
  • Cheema, AK, et al. (2018) Plasma Derived Exosomal Biomarkers of Exposure to Ionizing Radiation in Nonhuman Primates. Int J Mol Sci. 2018 Nov 1; 19(11). PM ID: 30388807
  • La Shu, S, et al. (2018) Metabolic reprogramming of stromal fibroblasts by melanoma exosome microRNA favours a pre-metastatic microenvironment. Sci Rep. 2018 Aug 27; 8(1):12905. PM ID: 30150674
  • Ruiz-de-León, MJ, et al. (2018) Lower expression of plasma-derived exosome miR-21 levels in HIV-1 elite controllers with decreasing CD4 T cell count. J Microbiol Immunol Infect. 2018 Aug 24;. PM ID: 30193823
  • Lin, M, et al. (2018) Exosomal neutral sphingomyelinase 1 suppresses hepatocellular carcinoma via decreasing the ratio of sphingomyelin/ceramide. FEBS J.. 2018 Aug 14;. PM ID: 30106227
  • Zhou, Y, et al. (2018) Exosomes from Endothelial Progenitor Cells Improve the Outcome of a Murine Model of Sepsis. Mol. Ther.. 2018 Feb 27;. PM ID: 29599080