ExoELISA-ULTRA Complete Kit (CD9 Detection)
- Sensitive—detect as little as 1 µg protein equivalent
- Fast—complete in less than 4-hours—no more overnight incubation
- Flexible—compatible with all major exosome isolation methods (e.g. SmartSEC, ExoQuick, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD9
- Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXEL-ULTRA-CD9-1 | ExoELISA-ULTRA Complete Kit (CD9 Detection) | 96 Reactions | $673 |
|
||||
Overview
Overview
Delivering ELISA-based exosome quantitation ULTRA fast
Improving on our popular ExoELISA Kits, the ExoELISA-ULTRA CD9 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.
Configured for detection of CD9, a common exosomal marker, ExoELISA-ULTRA CD9 is based on an ultra-sensitive direct capture colorimetric ELISA that is compatible with common biofluids. To enable quantitation of exosomes carrying CD9 in your target sample, the kit includes an internal standard calibrated to exosomes from a range of biofluids that have been analyzed by NTA analysis.
One ExoELISA-ULTRA CD9 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.
- Sensitive—detect as little as 1 µg protein equivalent
- Fast—complete in less than 4-hours—no more overnight incubation
- Flexible—compatible with all major exosome isolation methods (e.g. SmartSEC, ExoQuick, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD9
- Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
Choose the exosome quantitation method that’s best for your studies
| ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
|---|---|---|---|
| Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
| Detection method | Antibody | Enzymatic | Enzymatic |
| Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
| Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
| Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
| Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 ExoELISA-ULTRA GroEL | EXOCET | FluoroCet |
References
How It Works
Supporting Data
Supporting Data
The standard curve for ExoELISA-ULTRA CD9 shows robust linearity down to ~2 x 109 exosomes.
Figure 1. ExoELISA-ULTRA CD9 standard curve shows robust linearity down to ~2 x 109 exosomes. OD450 nm values of commonly used samples fall well within the standard curve for the assay.
FAQs
Resources
Citations
Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXEL-ULTRA-CD9-1 | ExoELISA-ULTRA Complete Kit (CD9 Detection) | 96 Reactions | $673 |
|
||||
Overview
Overview
Delivering ELISA-based exosome quantitation ULTRA fast
Improving on our popular ExoELISA Kits, the ExoELISA-ULTRA CD9 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.
Configured for detection of CD9, a common exosomal marker, ExoELISA-ULTRA CD9 is based on an ultra-sensitive direct capture colorimetric ELISA that is compatible with common biofluids. To enable quantitation of exosomes carrying CD9 in your target sample, the kit includes an internal standard calibrated to exosomes from a range of biofluids that have been analyzed by NTA analysis.
One ExoELISA-ULTRA CD9 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.
- Sensitive—detect as little as 1 µg protein equivalent
- Fast—complete in less than 4-hours—no more overnight incubation
- Flexible—compatible with all major exosome isolation methods (e.g. SmartSEC, ExoQuick, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD9
- Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
Choose the exosome quantitation method that’s best for your studies
| ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
|---|---|---|---|
| Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
| Detection method | Antibody | Enzymatic | Enzymatic |
| Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
| Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
| Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
| Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 ExoELISA-ULTRA GroEL | EXOCET | FluoroCet |
References
How It Works
Supporting Data
Supporting Data
The standard curve for ExoELISA-ULTRA CD9 shows robust linearity down to ~2 x 109 exosomes.
Figure 1. ExoELISA-ULTRA CD9 standard curve shows robust linearity down to ~2 x 109 exosomes. OD450 nm values of commonly used samples fall well within the standard curve for the assay.