ExoELISA-ULTRA Complete Kit (CD63 Detection)

Overview

Delivering ELISA-based exosome quantitation ULTRA fast

Improving on our popular ExoELISA^™ Kits, the ExoELISA-ULTRA CD63 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.

Currently configured for detection of CD63, a widely recognized and popular exosomal marker¹, ExoELISA-ULTRA CD63 is based on an ultra-sensitive, direct capture, colorimetric ELISA assay that is compatible with nearly all biofluids. The ExoELISA-ULTRA CD63 Kit comes with an internal standard calibrated to exosomes from a range of biofluids. Calibration is achieved by NanoSight analysis and enables quantitation of exosomes carrying CD63 in your target samples. One ExoELISA-ULTRA CD63 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.

• Sensitive—detect as little as 1 µg protein equivalent
• Fast—complete in less than 4-hours—no more overnight incubation
• Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick^®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
• Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
• Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications

1. Kowal, J., et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016. February 23. 113(8): E968–E977. PMCID: PMC4776515.