ExoELISA-ULTRA Complete Kit (CD63 Detection)
- Sensitive—detect as little as 1 µg protein equivalent
- Fast—complete in less than 4-hours—no more overnight incubation
- Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
- Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXEL-ULTRA-CD63-1 | ExoELISA-ULTRA Complete Kit (CD63 detection) | 96 Reactions | $673 |
|
Overview
Overview
Delivering ELISA-based exosome quantitation ULTRA fast
Improving on our popular ExoELISA™ Kits, the ExoELISA-ULTRA CD63 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.
Currently configured for detection of CD63, a widely recognized and popular exosomal marker1, ExoELISA-ULTRA CD63 is based on an ultra-sensitive, direct capture, colorimetric ELISA assay that is compatible with nearly all biofluids. The ExoELISA-ULTRA CD63 Kit comes with an internal standard calibrated to exosomes from a range of biofluids. Calibration is achieved by NanoSight analysis and enables quantitation of exosomes carrying CD63 in your target samples. One ExoELISA-ULTRA CD63 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.
- Sensitive—detect as little as 1 µg protein equivalent
- Fast—complete in less than 4-hours—no more overnight incubation
- Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
- Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
---|---|---|---|
Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
Detection method | Antibody | Enzymatic | Enzymatic |
Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 ExoELISA-ULTRA GroEL | EXOCET | FluoroCet |
- Kowal, J., et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016. February 23. 113(8): E968–E977. PMCID: PMC4776515.
References
How It Works
Supporting Data
FAQs
Related Products
Resources
Citations
-
Han, D, et al. (2024) Current Technology for Production, Isolation, and Quality Control of Extracellular Vesicles. Biomedical Applications of Extracellular Vesicles. 2024;:117-146. Link: Biomedical Applications of Extracellular Vesicles
-
Bhagwan Valjee, R, et al. (2024) Investigation of exosomal tetraspanin profile in sepsis patients as a promising diagnostic biomarker. Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals. 2024;:1-12. PM ID: 38354024
-
Johnston, J, et al. (2024) Stimulative piezoelectric nanofibrous scaffolds for enhanced small extracellular vesicle production in 3D cultures. bioRxiv : the preprint server for biology. 2024;. PM ID: 38659930
-
Robert, M, Jakhar, R & Crasta, K. (2024) Extracellular vesicles within a tumourigenic therapy-induced senescent tumour secretome are able to confer anti-cancer properties. bioRxiv. 2024;. Link: bioRxiv
-
Dias, T, et al. (2024) An electro-optical platform for the ultrasensitive detection of small extracellular vesicle sub-types and their protein epitope counts. iScience. 2024; 27(6):109866. PM ID: 38840839
-
Hudson, B. (2024) An in Vitro Investigation of Urothelial Cell Function in Response to Hypoxia and Pressure in Relation to Bladder Outlet Obstruction. Thesis. 2024;. Link: Thesis
-
Gao, H, et al. (2024) Extracellular vesicles from organoid-derived human retinal progenitor cells prevent lipid overload-induced retinal pigment epithelium injury by regulating fatty acid metabolism. Journal of extracellular vesicles. 2024; 13(1):e12401. PM ID: 38151470
-
Byappanahalli, A, et al. (2024) Extracellular vesicle mitochondrial DNA levels are associated with race and mitochondrial DNA haplogroup. iScience. 2024; 27(1):108724. Link: iScience
-
Nagao, Y, et al. (2024) Uterine leiomyosarcoma cell-derived extracellular vesicles induce the formation of cancer-associated fibroblasts. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 2024;:167103. PM ID: 38417460
-
Rajeev Kumar, S, Sakthiswary, R & Lokanathan, Y. (2024) Potential Therapeutic Application and Mechanism of Action of Stem Cell-Derived Extracellular Vesicles (EVs) in Systemic Lupus Erythematosus (SLE). International journal of molecular sciences. 2024; 25(4). PM ID: 38397121
-
Huang, Y, et al. (2024) Identification of a Serum Exosome-Derived lncRNA‒miRNA‒mRNA ceRNA Network in Patients with Endometriosis. Clinical and Experimental Obstetrics & Gynecology. 2024; 51(2):51. Link: Clinical and Experimental Obstetrics & Gynecology
-
Muraoka, A, et al. (2024) Small extracellular vesicles in follicular fluids for predicting reproductive outcomes in assisted reproductive technology. Communications medicine. 2024; 4(1):33. PM ID: 38418565
-
Liang, W, Najor, RH & Gustafsson, ÅB. (2024) Protocol to separate small and large extracellular vesicles from mouse and human cardiac tissues. STAR protocols. 2024; 5(1):102914. PM ID: 38386549
-
Otahal, A, et al. (2024) Culture of Hoffa fat pad mesenchymal stem/stromal cells on microcarrier suspension in vertical wheel bioreactor for extracellular vesicle production. Stem cell research & therapy. 2024; 15(1):61. PM ID: 38439108
-
Ibrahim, P, et al. (2024) Profiling Small RNA From Brain Extracellular Vesicles in Individuals With Depression. The international journal of neuropsychopharmacology. 2024; 27(3). PM ID: 38457375
-
Yung, C, et al. (2024) Neonatal enteroids absorb extracellular vesicles from human milk-fed infant digestive fluid. Journal of extracellular vesicles. 2024; 13(4):e12422. PM ID: 38602306
-
DeMarino, C, et al. (2024) Autophagy Deregulation in HIV-1-Infected Cells Increases Extracellular Vesicle Release and Contributes to TLR3 Activation. Viruses. 2024; 16(4). PM ID: 38675983
-
Parikh, R, et al. (2024) Recycled melanoma-secreted melanosomes regulate tumor-associated macrophage diversification. The EMBO journal. 2024;. PM ID: 38719996
-
Ye, X, et al. (2024) A novel function and mechanism of ischemia-induced retinal astrocyte-derived exosomes for RGC apoptosis of ischemic retinopathy. Molecular therapy. Nucleic acids. 2024; 35(2):102209. PM ID: 38831900
-
Hindle, J, et al. (2024) hTERT-Immortalized Mesenchymal Stem Cell-Derived Extracellular Vesicles: Large-Scale Manufacturing, Cargo Profiling, and Functional Effects in Retinal Epithelial Cells. Cells. 2024; 13(10). PM ID: 38786083
- See More
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXEL-ULTRA-CD63-1 | ExoELISA-ULTRA Complete Kit (CD63 detection) | 96 Reactions | $673 |
|
Overview
Overview
Delivering ELISA-based exosome quantitation ULTRA fast
Improving on our popular ExoELISA™ Kits, the ExoELISA-ULTRA CD63 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.
Currently configured for detection of CD63, a widely recognized and popular exosomal marker1, ExoELISA-ULTRA CD63 is based on an ultra-sensitive, direct capture, colorimetric ELISA assay that is compatible with nearly all biofluids. The ExoELISA-ULTRA CD63 Kit comes with an internal standard calibrated to exosomes from a range of biofluids. Calibration is achieved by NanoSight analysis and enables quantitation of exosomes carrying CD63 in your target samples. One ExoELISA-ULTRA CD63 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.
- Sensitive—detect as little as 1 µg protein equivalent
- Fast—complete in less than 4-hours—no more overnight incubation
- Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
- Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
---|---|---|---|
Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
Detection method | Antibody | Enzymatic | Enzymatic |
Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 ExoELISA-ULTRA GroEL | EXOCET | FluoroCet |
- Kowal, J., et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016. February 23. 113(8): E968–E977. PMCID: PMC4776515.
References
How It Works
FAQs
Citations
-
Han, D, et al. (2024) Current Technology for Production, Isolation, and Quality Control of Extracellular Vesicles. Biomedical Applications of Extracellular Vesicles. 2024;:117-146. Link: Biomedical Applications of Extracellular Vesicles
-
Bhagwan Valjee, R, et al. (2024) Investigation of exosomal tetraspanin profile in sepsis patients as a promising diagnostic biomarker. Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals. 2024;:1-12. PM ID: 38354024
-
Johnston, J, et al. (2024) Stimulative piezoelectric nanofibrous scaffolds for enhanced small extracellular vesicle production in 3D cultures. bioRxiv : the preprint server for biology. 2024;. PM ID: 38659930
-
Robert, M, Jakhar, R & Crasta, K. (2024) Extracellular vesicles within a tumourigenic therapy-induced senescent tumour secretome are able to confer anti-cancer properties. bioRxiv. 2024;. Link: bioRxiv
-
Dias, T, et al. (2024) An electro-optical platform for the ultrasensitive detection of small extracellular vesicle sub-types and their protein epitope counts. iScience. 2024; 27(6):109866. PM ID: 38840839
-
Hudson, B. (2024) An in Vitro Investigation of Urothelial Cell Function in Response to Hypoxia and Pressure in Relation to Bladder Outlet Obstruction. Thesis. 2024;. Link: Thesis
-
Gao, H, et al. (2024) Extracellular vesicles from organoid-derived human retinal progenitor cells prevent lipid overload-induced retinal pigment epithelium injury by regulating fatty acid metabolism. Journal of extracellular vesicles. 2024; 13(1):e12401. PM ID: 38151470
-
Byappanahalli, A, et al. (2024) Extracellular vesicle mitochondrial DNA levels are associated with race and mitochondrial DNA haplogroup. iScience. 2024; 27(1):108724. Link: iScience
-
Nagao, Y, et al. (2024) Uterine leiomyosarcoma cell-derived extracellular vesicles induce the formation of cancer-associated fibroblasts. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 2024;:167103. PM ID: 38417460
-
Rajeev Kumar, S, Sakthiswary, R & Lokanathan, Y. (2024) Potential Therapeutic Application and Mechanism of Action of Stem Cell-Derived Extracellular Vesicles (EVs) in Systemic Lupus Erythematosus (SLE). International journal of molecular sciences. 2024; 25(4). PM ID: 38397121
-
Huang, Y, et al. (2024) Identification of a Serum Exosome-Derived lncRNA‒miRNA‒mRNA ceRNA Network in Patients with Endometriosis. Clinical and Experimental Obstetrics & Gynecology. 2024; 51(2):51. Link: Clinical and Experimental Obstetrics & Gynecology
-
Muraoka, A, et al. (2024) Small extracellular vesicles in follicular fluids for predicting reproductive outcomes in assisted reproductive technology. Communications medicine. 2024; 4(1):33. PM ID: 38418565
-
Liang, W, Najor, RH & Gustafsson, ÅB. (2024) Protocol to separate small and large extracellular vesicles from mouse and human cardiac tissues. STAR protocols. 2024; 5(1):102914. PM ID: 38386549
-
Otahal, A, et al. (2024) Culture of Hoffa fat pad mesenchymal stem/stromal cells on microcarrier suspension in vertical wheel bioreactor for extracellular vesicle production. Stem cell research & therapy. 2024; 15(1):61. PM ID: 38439108
-
Ibrahim, P, et al. (2024) Profiling Small RNA From Brain Extracellular Vesicles in Individuals With Depression. The international journal of neuropsychopharmacology. 2024; 27(3). PM ID: 38457375
-
Yung, C, et al. (2024) Neonatal enteroids absorb extracellular vesicles from human milk-fed infant digestive fluid. Journal of extracellular vesicles. 2024; 13(4):e12422. PM ID: 38602306
-
DeMarino, C, et al. (2024) Autophagy Deregulation in HIV-1-Infected Cells Increases Extracellular Vesicle Release and Contributes to TLR3 Activation. Viruses. 2024; 16(4). PM ID: 38675983
-
Parikh, R, et al. (2024) Recycled melanoma-secreted melanosomes regulate tumor-associated macrophage diversification. The EMBO journal. 2024;. PM ID: 38719996
-
Ye, X, et al. (2024) A novel function and mechanism of ischemia-induced retinal astrocyte-derived exosomes for RGC apoptosis of ischemic retinopathy. Molecular therapy. Nucleic acids. 2024; 35(2):102209. PM ID: 38831900
-
Hindle, J, et al. (2024) hTERT-Immortalized Mesenchymal Stem Cell-Derived Extracellular Vesicles: Large-Scale Manufacturing, Cargo Profiling, and Functional Effects in Retinal Epithelial Cells. Cells. 2024; 13(10). PM ID: 38786083
- See More