ExoELISA-ULTRA Complete Kit (CD63 Detection)

Overview

Delivering ELISA-based exosome quantitation ULTRA fast

Improving on our popular ExoELISA^™ Kits, the ExoELISA-ULTRA CD63 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.

Currently configured for detection of CD63, a widely recognized and popular exosomal marker¹, ExoELISA-ULTRA CD63 is based on an ultra-sensitive, direct capture, colorimetric ELISA assay that is compatible with nearly all biofluids. The ExoELISA-ULTRA CD63 Kit comes with an internal standard calibrated to exosomes from a range of biofluids. Calibration is achieved by NanoSight analysis and enables quantitation of exosomes carrying CD63 in your target samples. One ExoELISA-ULTRA CD63 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.

• Sensitive—detect as little as 1 µg protein equivalent
• Fast—complete in less than 4-hours—no more overnight incubation
• Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick^®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human, mouse, and rat
• Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
• Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications

Choose the exosome quantitation method that’s best for your studies

REFERENCES

1. Kowal, J., et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016. February 23. 113(8): E968–E977. PMCID: PMC4776515.

Supporting Data

The standard curve for ExoELISA-ULTRA CD63 shows robust linearity down to ~1 x 10⁹ exosomes.

Resources

Citations

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• Freitas, D, et al. (2019) Different isolation approaches lead to diverse glycosylated extracellular vesicle populations. J Extracell Vesicles. 2019 Jun 3; 8(1):1621131. PM ID: 31236201
• Sung, PS, Huang, TF & Hsieh, SL. (2019) Extracellular vesicles from CLEC2-activated platelets enhance dengue virus-induced lethality via CLEC5A/TLR2. Nat Commun. 2019 Jun 3; 10(1):2402. PM ID: 31160588
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• Guo, DF, et al. (2019) The BBSome in POMC and AgRP Neurons is Necessary for Body Weight Regulation and Sorting of Metabolic Receptors. Diabetes. 2019 May 24;. PM ID: 31127052
• Maurel, M, et al. (2019) Control of anterior GRadient 2 (AGR2) dimerization links endoplasmic reticulum proteostasis to inflammation. EMBO Mol Med. 2019 Apr 30;. PM ID: 31040128
• Parry, HA, et al. (2019) Bovine Milk Extracellular Vesicles (EVs) Modification Elicits Skeletal Muscle Growth in Rats. Front Physiol. 2019 Apr 16; 10:436. PM ID: 31040795
• Chen, P, et al. (2019) Desktop-stereolithography 3D printing of a radially oriented extracellular matrix/mesenchymal stem cell exosome bioink for osteochondral defect regeneration. Theranostics. 2019 Apr 13; 9(9):2439-2459. PM ID: 31131046
• Reiter, K, et al. (2019) Separation of virus-like particles and extracellular vesicles by flow-through and heparin affinity chromatography. J Chromatogr A. 2019 Mar 15; 1588:77-84. PM ID: 30616980
• Tobón-Arroyave, SI, et al. (2019) Decreased salivary concentration of CD9 and CD81 exosome-related tetraspanins may be associated with the periodontal clinical status. J. Clin. Periodontol.. 2019 Mar 2;. PM ID: 30825338
• Kim, JH, Lee, CH & Lee, SW. (2019) Exosomal Transmission of MicroRNA from HCV Replicating Cells Stimulates Transdifferentiation in Hepatic Stellate Cells. Mol Ther Nucleic Acids. 2019 Mar 1; 14:483-497. PM ID: 30753992
• Capello, M, et al. (2019) Exosomes harbor B cell targets in pancreatic adenocarcinoma and exert decoy function against complement-mediated cytotoxicity. Nat Commun. 2019 Jan 16; 10(1):254. PM ID: 30651550
• Zlotogorski-Hurvitz, A, et al. (2019) FTIR-based spectrum of salivary exosomes coupled with computational-aided discriminating analysis in the diagnosis of oral cancer. J. Cancer Res. Clin. Oncol.. 2019 Jan 3;. PM ID: 30603907
• Baryeh, K. (2019) Development of Quantitative Lateral Flow Strip Biosensors for the Detection of Cancer Biomarkers. Thesis. ;. Link: Thesis
• Cheema, AK, et al. (2018) Plasma Derived Exosomal Biomarkers of Exposure to Ionizing Radiation in Nonhuman Primates. Int J Mol Sci. 2018 Nov 1; 19(11). PM ID: 30388807
• La Shu, S, et al. (2018) Metabolic reprogramming of stromal fibroblasts by melanoma exosome microRNA favours a pre-metastatic microenvironment. Sci Rep. 2018 Aug 27; 8(1):12905. PM ID: 30150674
• Ruiz-de-León, MJ, et al. (2018) Lower expression of plasma-derived exosome miR-21 levels in HIV-1 elite controllers with decreasing CD4 T cell count. J Microbiol Immunol Infect. 2018 Aug 24;. PM ID: 30193823
• Lin, M, et al. (2018) Exosomal neutral sphingomyelinase 1 suppresses hepatocellular carcinoma via decreasing the ratio of sphingomyelin/ceramide. FEBS J.. 2018 Aug 14;. PM ID: 30106227
• Zhou, Y, et al. (2018) Exosomes from Endothelial Progenitor Cells Improve the Outcome of a Murine Model of Sepsis. Mol. Ther.. 2018 Feb 27;. PM ID: 29599080