ExoELISA-ULTRA Complete Kit (CD63 Detection)
With a 4-hour total assay time, this sensitive ELISA-based assay speeds quantitation of exosomes from most biofluids
- Sensitive—detect as little as 1 µg protein equivalent
- Fast—complete in less than 4-hours—no more overnight incubation
- Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
- Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
Overview
Delivering ELISA-based exosome quantitation ULTRA fast
Improving on our popular ExoELISA™ Kits, the ExoELISA-ULTRA CD63 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.
Currently configured for detection of CD63, a widely recognized and popular exosomal marker1, ExoELISA-ULTRA CD63 is based on an ultra-sensitive, direct capture, colorimetric ELISA assay that is compatible with nearly all biofluids. The ExoELISA-ULTRA CD63 Kit comes with an internal standard calibrated to exosomes from a range of biofluids. Calibration is achieved by NanoSight analysis and enables quantitation of exosomes carrying CD63 in your target samples. One ExoELISA-ULTRA CD63 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.
- Sensitive—detect as little as 1 µg protein equivalent
- Fast—complete in less than 4-hours—no more overnight incubation
- Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
- Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
Choose the exosome quantitation method that’s best for your studies
ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 |
ExoELISA CD9 ExoELISA CD63 ExoELISA CD81 |
EXOCET | FluoroCet | |
---|---|---|---|---|
Use | For fast and sensitive antibody-based quantitation of exosomes | For sensitive quantitation of exosomes when time and input sample are not limiting | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
Detection method | Antibody | Antibody | Enzymatic | Enzymatic |
Quantitation chemistry | Enzymatic (HRP) | Enzymatic (HRP) | Colorimetric | Fluorescent |
Total protocol time | 4 hours (no overnight incubation) | 24 hours | 20 min | 60 min |
Input sample amount (protein equivalent) | 1 – 200 µg | >500 µg | 50 µg | <1 µg |
Learn More | ExoELISA-ULTRA CD63 | ExoELISA CD9 ExoELISA CD63 ExoELISA CD81 |
EXOCET | FluoroCet |
REFERENCES
- Kowal, J., et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016. February 23. 113(8): E968–E977. PMCID: PMC4776515.
Citations
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- Freitas, D, et al. (2019) Different isolation approaches lead to diverse glycosylated extracellular vesicle populations. J Extracell Vesicles. 2019 Jun 3; 8(1):1621131. PM ID: 31236201
- Sung, PS, Huang, TF & Hsieh, SL. (2019) Extracellular vesicles from CLEC2-activated platelets enhance dengue virus-induced lethality via CLEC5A/TLR2. Nat Commun. 2019 Jun 3; 10(1):2402. PM ID: 31160588
- Cui, Y, et al. (2019) EPC-derived exosomes promote osteoclastogenesis through LncRNA-MALAT1. J. Cell. Mol. Med.. 2019 Jun 1; 23(6):3843-3854. PM ID: 31025509
- Guo, DF, et al. (2019) The BBSome in POMC and AgRP Neurons is Necessary for Body Weight Regulation and Sorting of Metabolic Receptors. Diabetes. 2019 May 24;. PM ID: 31127052
- Maurel, M, et al. (2019) Control of anterior GRadient 2 (AGR2) dimerization links endoplasmic reticulum proteostasis to inflammation. EMBO Mol Med. 2019 Apr 30;. PM ID: 31040128
- Parry, HA, et al. (2019) Bovine Milk Extracellular Vesicles (EVs) Modification Elicits Skeletal Muscle Growth in Rats. Front Physiol. 2019 Apr 16; 10:436. PM ID: 31040795
- Chen, P, et al. (2019) Desktop-stereolithography 3D printing of a radially oriented extracellular matrix/mesenchymal stem cell exosome bioink for osteochondral defect regeneration. Theranostics. 2019 Apr 13; 9(9):2439-2459. PM ID: 31131046
- Reiter, K, et al. (2019) Separation of virus-like particles and extracellular vesicles by flow-through and heparin affinity chromatography. J Chromatogr A. 2019 Mar 15; 1588:77-84. PM ID: 30616980
- Tobón-Arroyave, SI, et al. (2019) Decreased salivary concentration of CD9 and CD81 exosome-related tetraspanins may be associated with the periodontal clinical status. J. Clin. Periodontol.. 2019 Mar 2;. PM ID: 30825338
- Kim, JH, Lee, CH & Lee, SW. (2019) Exosomal Transmission of MicroRNA from HCV Replicating Cells Stimulates Transdifferentiation in Hepatic Stellate Cells. Mol Ther Nucleic Acids. 2019 Mar 1; 14:483-497. PM ID: 30753992
- Capello, M, et al. (2019) Exosomes harbor B cell targets in pancreatic adenocarcinoma and exert decoy function against complement-mediated cytotoxicity. Nat Commun. 2019 Jan 16; 10(1):254. PM ID: 30651550
- Zlotogorski-Hurvitz, A, et al. (2019) FTIR-based spectrum of salivary exosomes coupled with computational-aided discriminating analysis in the diagnosis of oral cancer. J. Cancer Res. Clin. Oncol.. 2019 Jan 3;. PM ID: 30603907
- Baryeh, K. (2019) Development of Quantitative Lateral Flow Strip Biosensors for the Detection of Cancer Biomarkers. Thesis. ;. Link: Thesis
- Cheema, AK, et al. (2018) Plasma Derived Exosomal Biomarkers of Exposure to Ionizing Radiation in Nonhuman Primates. Int J Mol Sci. 2018 Nov 1; 19(11). PM ID: 30388807
- La Shu, S, et al. (2018) Metabolic reprogramming of stromal fibroblasts by melanoma exosome microRNA favours a pre-metastatic microenvironment. Sci Rep. 2018 Aug 27; 8(1):12905. PM ID: 30150674
- Ruiz-de-León, MJ, et al. (2018) Lower expression of plasma-derived exosome miR-21 levels in HIV-1 elite controllers with decreasing CD4 T cell count. J Microbiol Immunol Infect. 2018 Aug 24;. PM ID: 30193823
- Lin, M, et al. (2018) Exosomal neutral sphingomyelinase 1 suppresses hepatocellular carcinoma via decreasing the ratio of sphingomyelin/ceramide. FEBS J.. 2018 Aug 14;. PM ID: 30106227
- Zhou, Y, et al. (2018) Exosomes from Endothelial Progenitor Cells Improve the Outcome of a Murine Model of Sepsis. Mol. Ther.. 2018 Feb 27;. PM ID: 29599080