TransDux™ MAX Lentivirus Transduction Reagent
- Efficient—up to 8-fold greater transduction efficiency than polybrene, and 4-fold greater efficiency than standard TransDux reagent as measured by qPCR*
- Easy-to-use—kit format requires minimal hands-on time (<5 minutes)
- Non-toxic—no need to change out media after infection
- Versatile—works with all types of packaged lentivirus
- Flexible—enhances transduction efficiency in a wide variety of cell lines
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
LV860A-1 | TransDux MAX Lentivirus Transduction Enhancer | 100 Transductions | $405 |
|
Overview
Overview
Be confident in your gene delivery with TransDux MAX™
Whether you’re working with difficult-to-transduce cells like primary T-cells or just want to ensure high transduction efficiency, SBI’s TransDux™ reagents are ready to deliver. Our newer TransDux MAX Lentiviral Transduction Reagent can increase transduction efficiencies by up to 8-fold compared to polybrene, while our original TransDux formulation (Cat# LV850A-1) is still available for researchers who are not yet ready to make the change.
Ensure reliable and efficient gene delivery with TransDux MAX.
- Efficient—up to 8-fold greater transduction efficiency than polybrene, and 4-fold greater efficiency than standard TransDux reagent as measured by qPCR*
- Easy-to-use—kit format requires minimal hands-on time (<5 minutes)
- Non-toxic—no need to change out media after infection
- Versatile—works with all types of packaged lentivirus
- Flexible—enhances transduction efficiency in a wide variety of cell lines
- Broadly compatible—does not interfere with downstream gene expression or functional assays
*Based on infection of HT1080 cells with the pre-packaged virus format of SBI’s BLIV 2.0 reporter MSCV-Luciferase-EF1a-copGFP-T2A-Puro (Cat# BLIV713VA-1) and the appropriate conditions for polybrene, TransDux, and TransDux MAX. Virus transduction & integration efficiency was measured using SBI’s Global UltraRapid™ Titering Kit (Cat #LV961A-1)
References
How It Works
How It Works
Take your transduction efficiencies to a whole new level
TransDux MAX comes in an easy-to-use, two component format (TransDux and MAX Enhancer) good for 100 transduction reactions*. Simply mix both reagents into conditioned media from target cells (Figure 1), and infect with your lentivirus of choice for a higher level of transduction efficiency.
*One reaction is defined as treating 500 µl of conditioned media in a single well of 24-well plate
Supporting Data
FAQs
- Efficient: up to 8-fold greater transduction efficiency than polybrene and 4-fold greater efficiency than standard TransDux reagent
- Easy-to-use: requires minimal hands-on time (<5 minutes)
- Non-toxic: no need to change out media after infection
- Versatile: works with all types of packaged lentivirus
- Flexible: enhances transduction efficiency in a wide variety of cell lines
- Mix both TransDux and MAX Enhancer reagents into conditioned media from target cells.
- Infect the cells with your lentivirus of choice.
- Observe higher transduction efficiency compared to other methods.
- Is TransDux™ MAX compatible with various cell types and lentivectors?
- Yes, TransDux™ MAX works with a wide variety of cell lines and is compatible with all types of packaged lentivirus. It also works well with hard-to-transduce lentivectors.
Related Products
Resources
Citations
-
Budagyan, K, Cannon, AC & Chernoff, J. (2024) A Facile Method to Append a Bio-ID Tag to Endogenous Mutant Kras Alleles. Methods in molecular biology (Clifton, N.J.). 2024; 2797:351-362. PM ID: 38570472
-
Jiang, C, et al. (2024) General anaesthetics reduce acute lymphoblastic leukaemia malignancies in vitro and in vivo via CXCR4 and osteopontin mediated mechanisms. F1000Research. 2024;. Link: F1000Research
-
Werner, MS, et al. (2024) Adeno-associated virus-mediated trastuzumab delivery to the central nervous system for human epidermal growth factor receptor 2+ brain metastasis. Cancer gene therapy. 2024;. PM ID: 38480976
-
Rigaux, E, et al. (2023) Budget-Friendly Generation, Biochemical Analyses, and Lentiviral Transduction of Patient-Derived Colon Organoids. Current protocols. 2023; 3(12):e943. PM ID: 38058263
-
Rialdi, A, et al. (2023) WNTinib is a multi-kinase inhibitor with specificity against β-catenin mutant hepatocellular carcinoma. Nature cancer. 2023; 4(8):1157-1175. PM ID: 37537299
-
Tejeda, MR. (2023) Direct Reprogramming of Human Cells to a Cardiomyocyte-Like State. Thesis. 2023;. Link: Thesis
-
Guilz, N, et al. (2023) Replication stress in activated human NK cells induces sensitivity to apoptosis. bioRxiv. 2023;. Link: bioRxiv
-
Reddy-Vari, H, et al. (2023) Increased expression of miR146a dysregulates TLR2-induced HBD2 in airway epithelial cells from patients with COPD. ERJ open research. 2023; 9(3). PM ID: 37228294
-
Kits, GFBAA. (2023) Montana Molecular. Thesis. 2023;. Link: Thesis
-
Jiang, C, et al. (2022) General anaesthetics reduce acute lymphoblastic leukaemia cell migration and homing in vitro and in vivo via CXCR4 and osteopontin mediated mechanisms. F1000Research. 2022; 11:1491. Link: F1000Research
-
Choe, Y, et al. (2022) Heterotypic cell-in-cell structures between cancer and NK cells is associated with enhanced anti-cancer drug resistance. iScience. 2022;:105017. Link: iScience
-
Reddy-Vari, H, et al. (2022) Increased expression of miR146a dysregulates TLR2 signaling in airway epithelial cells from patients with chronic obstructive pulmonary disease. Research Square. 2022;. Link: Research Square
-
Antonia, RJ, et al. (2022) STAT3 regulates inflammatory cytokine production downstream of TNFR1 by inducing expression of TNFAIP3/A20. Journal of cellular and molecular medicine. 2022;. PM ID: 35841281
-
Antonia, R, et al. (2022) STAT3 regulates cytokine production downstream of TNFR1 in part by inducing expression of TNFAIP3/A20. bioRxiv. 2022;. Link: bioRxiv
-
Nguyen, LNT, et al. (2022) TRF2 inhibition rather than telomerase disruption drives CD4 T cell dysfunction during chronic viral infection. Journal of cell science. 2022;. PM ID: 35660868
-
Fischietti, M, et al. (2022) SLFN11 negatively regulates non-canonical NFkB signaling to promote glioblastoma progression. Cancer research communications. 2022; 2(9):966-978. PM ID: 36382088
-
Duffy, M. (2022) Targeting CTNNB1-Mutant Hepatocellular Carcinoma with a Novel Kinase Inhibitor. Thesis. 2022;. Link: Thesis
-
De Silva, D, et al. (2021) Robust T cell activation requires an eIF3-driven burst in T cell receptor translation. eLife. 2021; 10. PM ID: 34970966
-
Budagyan, K & Chernoff, J. (2021) A Facile Method to Engineer Mutant Kras Alleles in an Isogenic Cell Background. Methods in molecular biology (Clifton, N.J.). 2021; 2262:323-334. PM ID: 33977487
-
Hiraike, Y, et al. (2020) NFIA differentially controls adipogenic and myogenic gene program through distinct pathways to ensure brown and beige adipocyte differentiation. PLoS Genet. 2020; 16(9):e1009044. PM ID: 32991581
- See More
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
LV860A-1 | TransDux MAX Lentivirus Transduction Enhancer | 100 Transductions | $405 |
|
Overview
Overview
Be confident in your gene delivery with TransDux MAX™
Whether you’re working with difficult-to-transduce cells like primary T-cells or just want to ensure high transduction efficiency, SBI’s TransDux™ reagents are ready to deliver. Our newer TransDux MAX Lentiviral Transduction Reagent can increase transduction efficiencies by up to 8-fold compared to polybrene, while our original TransDux formulation (Cat# LV850A-1) is still available for researchers who are not yet ready to make the change.
Ensure reliable and efficient gene delivery with TransDux MAX.
- Efficient—up to 8-fold greater transduction efficiency than polybrene, and 4-fold greater efficiency than standard TransDux reagent as measured by qPCR*
- Easy-to-use—kit format requires minimal hands-on time (<5 minutes)
- Non-toxic—no need to change out media after infection
- Versatile—works with all types of packaged lentivirus
- Flexible—enhances transduction efficiency in a wide variety of cell lines
- Broadly compatible—does not interfere with downstream gene expression or functional assays
*Based on infection of HT1080 cells with the pre-packaged virus format of SBI’s BLIV 2.0 reporter MSCV-Luciferase-EF1a-copGFP-T2A-Puro (Cat# BLIV713VA-1) and the appropriate conditions for polybrene, TransDux, and TransDux MAX. Virus transduction & integration efficiency was measured using SBI’s Global UltraRapid™ Titering Kit (Cat #LV961A-1)
References
How It Works
How It Works
Take your transduction efficiencies to a whole new level
TransDux MAX comes in an easy-to-use, two component format (TransDux and MAX Enhancer) good for 100 transduction reactions*. Simply mix both reagents into conditioned media from target cells (Figure 1), and infect with your lentivirus of choice for a higher level of transduction efficiency.
*One reaction is defined as treating 500 µl of conditioned media in a single well of 24-well plate
FAQs
- Efficient: up to 8-fold greater transduction efficiency than polybrene and 4-fold greater efficiency than standard TransDux reagent
- Easy-to-use: requires minimal hands-on time (<5 minutes)
- Non-toxic: no need to change out media after infection
- Versatile: works with all types of packaged lentivirus
- Flexible: enhances transduction efficiency in a wide variety of cell lines
- Mix both TransDux and MAX Enhancer reagents into conditioned media from target cells.
- Infect the cells with your lentivirus of choice.
- Observe higher transduction efficiency compared to other methods.
- Is TransDux™ MAX compatible with various cell types and lentivectors?
- Yes, TransDux™ MAX works with a wide variety of cell lines and is compatible with all types of packaged lentivirus. It also works well with hard-to-transduce lentivectors.
Citations
-
Budagyan, K, Cannon, AC & Chernoff, J. (2024) A Facile Method to Append a Bio-ID Tag to Endogenous Mutant Kras Alleles. Methods in molecular biology (Clifton, N.J.). 2024; 2797:351-362. PM ID: 38570472
-
Jiang, C, et al. (2024) General anaesthetics reduce acute lymphoblastic leukaemia malignancies in vitro and in vivo via CXCR4 and osteopontin mediated mechanisms. F1000Research. 2024;. Link: F1000Research
-
Werner, MS, et al. (2024) Adeno-associated virus-mediated trastuzumab delivery to the central nervous system for human epidermal growth factor receptor 2+ brain metastasis. Cancer gene therapy. 2024;. PM ID: 38480976
-
Rigaux, E, et al. (2023) Budget-Friendly Generation, Biochemical Analyses, and Lentiviral Transduction of Patient-Derived Colon Organoids. Current protocols. 2023; 3(12):e943. PM ID: 38058263
-
Rialdi, A, et al. (2023) WNTinib is a multi-kinase inhibitor with specificity against β-catenin mutant hepatocellular carcinoma. Nature cancer. 2023; 4(8):1157-1175. PM ID: 37537299
-
Tejeda, MR. (2023) Direct Reprogramming of Human Cells to a Cardiomyocyte-Like State. Thesis. 2023;. Link: Thesis
-
Guilz, N, et al. (2023) Replication stress in activated human NK cells induces sensitivity to apoptosis. bioRxiv. 2023;. Link: bioRxiv
-
Reddy-Vari, H, et al. (2023) Increased expression of miR146a dysregulates TLR2-induced HBD2 in airway epithelial cells from patients with COPD. ERJ open research. 2023; 9(3). PM ID: 37228294
-
Kits, GFBAA. (2023) Montana Molecular. Thesis. 2023;. Link: Thesis
-
Jiang, C, et al. (2022) General anaesthetics reduce acute lymphoblastic leukaemia cell migration and homing in vitro and in vivo via CXCR4 and osteopontin mediated mechanisms. F1000Research. 2022; 11:1491. Link: F1000Research
-
Choe, Y, et al. (2022) Heterotypic cell-in-cell structures between cancer and NK cells is associated with enhanced anti-cancer drug resistance. iScience. 2022;:105017. Link: iScience
-
Reddy-Vari, H, et al. (2022) Increased expression of miR146a dysregulates TLR2 signaling in airway epithelial cells from patients with chronic obstructive pulmonary disease. Research Square. 2022;. Link: Research Square
-
Antonia, RJ, et al. (2022) STAT3 regulates inflammatory cytokine production downstream of TNFR1 by inducing expression of TNFAIP3/A20. Journal of cellular and molecular medicine. 2022;. PM ID: 35841281
-
Antonia, R, et al. (2022) STAT3 regulates cytokine production downstream of TNFR1 in part by inducing expression of TNFAIP3/A20. bioRxiv. 2022;. Link: bioRxiv
-
Nguyen, LNT, et al. (2022) TRF2 inhibition rather than telomerase disruption drives CD4 T cell dysfunction during chronic viral infection. Journal of cell science. 2022;. PM ID: 35660868
-
Fischietti, M, et al. (2022) SLFN11 negatively regulates non-canonical NFkB signaling to promote glioblastoma progression. Cancer research communications. 2022; 2(9):966-978. PM ID: 36382088
-
Duffy, M. (2022) Targeting CTNNB1-Mutant Hepatocellular Carcinoma with a Novel Kinase Inhibitor. Thesis. 2022;. Link: Thesis
-
De Silva, D, et al. (2021) Robust T cell activation requires an eIF3-driven burst in T cell receptor translation. eLife. 2021; 10. PM ID: 34970966
-
Budagyan, K & Chernoff, J. (2021) A Facile Method to Engineer Mutant Kras Alleles in an Isogenic Cell Background. Methods in molecular biology (Clifton, N.J.). 2021; 2262:323-334. PM ID: 33977487
-
Hiraike, Y, et al. (2020) NFIA differentially controls adipogenic and myogenic gene program through distinct pathways to ensure brown and beige adipocyte differentiation. PLoS Genet. 2020; 16(9):e1009044. PM ID: 32991581
- See More