EVery EV RNA Isolation Kit
- Move quickly and confidently with exoRNA isolation that's high-yield and complete in <30 minutes.
- Find what others miss when you capture every RNA with EVery
- Achieve phenol-level yields with a safer column-based method
- Get more RNA for each downstream reaction with EVery's small-volume elutions
- Ensure delivery of highly pure RNA by using the included DNaseI
- Compatible with most downstream applications, including RNA-seq and miRNA profiling
- Works with EVs isolated with commonly-used methods, such as ExoQuick, SmartSEC, and ultracentrifugation.
- New column dimensions work just as smoothly for RNA isolation from all types of EV sample, irrespective of upstream EV isolation methods.
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| Every100B-1 | EVery EV RNA Isolation Kit | 20 Preps | $427 |
|
||||
Overview
Overview
Discover more when you capture total EVery
EV samples vary drastically in their purity and concentration. Some EV samples from sera of diseased subjects and plasma bring up the challenge of heavy load of contaminating proteins that can potentially lead to column clogging thus lower yields. New column dimensions work just as smoothly for RNA isolation from all types of EV samples, irrespective of upstream EV isolation methods.
Overcoming many of the other challenges with RNA isolation from extracellular vesicles (EVs), the EVery EV RNA Isolation Kit is able to capture total EV RNA, including small RNAs. EVery is effective even with low amounts of input RNA and is capable of delivering high yields of highly pure RNA. Because the RNA elutes in a small sample volume, thus generating a highly concentrated prep, you can increase the amount of RNA used in a single downstream reaction for better data coverage quality.
- Move quickly and confidently with exoRNA isolation that's high-yield and complete in <30 minutes.
- Find what others miss when you capture every RNA with EVery
- Achieve phenol-level yields with a safer column-based method
- Get more RNA for each downstream reaction with EVery's small-volume elutions
- Ensure delivery of highly pure RNA by using the included DNaseI
- Compatible with most downstream applications, including RNA-seq and miRNA profiling
- Works with EVs isolated with commonly-used methods, such as ExoQuick, SmartSEC, and ultracentrifugation.
The EVery EV RNA Isolation Kit can be used with EVs isolated by commonly used methods, including ExoQuick, SmartSEC, and ultracentrifugation, and comes with sufficient reagents to perform 20 purification reactions. EVery technology is also available bundled with SBI's powerful EV isolation technologies as well as a EVery cDNA Synthesis Kit (Table 1).
Table 1. EVery EV RNA Isolation and cDNA Synthesis Products
| Cat. # | Product description |
|---|---|
| EVery100B-1 | EVery EV RNA Isolation Kit |
| EVery200B-1 | EVery cDNA Synthesis Kit |
References
How It Works
How It Works
See the quick and easy EVery workflow
The EVery EV RNA Isolation Kit delivers high yields of highly concentrated RNA from already isolated EVs. The column-based workflow is easy to implement and can be completed in less than 30 minutes (Figure 1).
Figure 1. The quick and easy EVery EV RNA Isolation workflow.
Supporting Data
Supporting Data
EVery captures EVerything
To demonstrate the ability of the EVery EV RNA Isolation Kit to capture the full range of RNAs, we used the kit to isolate RNA from 10,000 cells (Figure 2, lane 1), from EVs that were isolated from 250 µL of serum using SmartSEC Single (Figure 2, lane 2), and from buffer spiked with 0.1 pmol of Cel-miR-39 (Figure 2, lane 3). The high quality of the isolated RNA can be seen in lane 1, where the RNA integrity number (RIN) is 9.9 and the 28S/18S RNA ratio is 1.5. The multiple bands in lane 2 demonstrate that EVery captures RNAs of different lengths—EVerything—from EVs with no apparent bias or size preference. The strong signal from the spiked-in miRNA in lane 3 demonstrates the good recovery of even small RNAs.
Figure 2. EVery captures EVerything.
The compatibility of multiple EV isolation techniques with EVery and the excellent size distribution of RNAs isolated from those EVs is shown in Figure 3.
Figure 3. EVery is compatible with EVs isolated using ExoQuick Ultra and SmartSEC Single.
EVery delivers similar amounts of RNA as phenol-based methods
To demonstrate the excellent RNA yields obtained with EVery, we isolated EVs from 250 µL of serum using SmartSEC Single, spiked in 0.1 pmol of Cel-miR-39, and used both EVery and a phenol-based kit to isolate RNA. The isolated RNA was reverse transcribed using the EVery cDNA Synthesis Kit and the copy number of Cel-miR-39 measured (Figure 4). TheEVery EV RNA Isolation Kit delivered similar levels of Cel-miR-39 as the phenol-based method.
Figure 4. EVery is EVery bit as good as phenol.
EVery efficiently isolates mRNA
We used the EVery EV RNA Isolation kit to isolate mRNA from cells overexpressing eGFP (Figure 5). Robust levels of eGFP mRNA are recovered when cells are overexpressing eGFP.
Figure 5. EVery efficiently isolates longer RNAs like mRNA.
miRNA isolated using EVery can be used for miRNA profiling and RNA-seq
We isolated EVs from 250 µL of normal or breast cancer patient serum using SmartSEC Single and used EVery EV RNA Isolation Kit and EVery cDNA Synthesis Kit to isolate and reverse transcribe EV RNAs for miRNA profiling using EVery miRNome Profiler for Human Serum and Plasma (EVery500B-1). Distinct miRNA expression profiles of breast cancer patient serum compared to normal serum are shown in Figure 6. Differential expression of selected breast cancer associated miRNA markers were further confirmed by qPCR (Figure 7).
Figure 6. EVery family of products generate high-quality cDNA suitable for miRNA profiling.
Figure 7. Differential expression of selected miRNAs in normal vs. breast cancer serum confirmed by qPCR. Data normalized with global mean. Expression of miR-451a, miR-21-5p, miR-16-5p and miR-103a are significantly upregulated, while expression of the tumor suppressor miR-124-3p is significantly downregulated in breast cancer serum compared to normal serum.
We were also able to show robust, successful RNA-seq runs using RNA isolated from EVs with EVery (Table 2). All three EV isolation methods tested generated high-quality RNA-seq data.
Table 2. Successful RNA-seq with EVery-isolated EV RNA
EV Isolation Method | Amount of RNA isolated (ng) | Number of reads | FAST-QC |
|---|---|---|---|
4.3 | 53,349,528 | Passed |
|
5.4 | 107,154,128 | Passed |
|
5.2 | 98,886,924 | Passed |
FAQs
Resources
Citations
Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| Every100B-1 | EVery EV RNA Isolation Kit | 20 Preps | $427 |
|
||||
Overview
Overview
Discover more when you capture total EVery
EV samples vary drastically in their purity and concentration. Some EV samples from sera of diseased subjects and plasma bring up the challenge of heavy load of contaminating proteins that can potentially lead to column clogging thus lower yields. New column dimensions work just as smoothly for RNA isolation from all types of EV samples, irrespective of upstream EV isolation methods.
Overcoming many of the other challenges with RNA isolation from extracellular vesicles (EVs), the EVery EV RNA Isolation Kit is able to capture total EV RNA, including small RNAs. EVery is effective even with low amounts of input RNA and is capable of delivering high yields of highly pure RNA. Because the RNA elutes in a small sample volume, thus generating a highly concentrated prep, you can increase the amount of RNA used in a single downstream reaction for better data coverage quality.
- Move quickly and confidently with exoRNA isolation that's high-yield and complete in <30 minutes.
- Find what others miss when you capture every RNA with EVery
- Achieve phenol-level yields with a safer column-based method
- Get more RNA for each downstream reaction with EVery's small-volume elutions
- Ensure delivery of highly pure RNA by using the included DNaseI
- Compatible with most downstream applications, including RNA-seq and miRNA profiling
- Works with EVs isolated with commonly-used methods, such as ExoQuick, SmartSEC, and ultracentrifugation.
The EVery EV RNA Isolation Kit can be used with EVs isolated by commonly used methods, including ExoQuick, SmartSEC, and ultracentrifugation, and comes with sufficient reagents to perform 20 purification reactions. EVery technology is also available bundled with SBI's powerful EV isolation technologies as well as a EVery cDNA Synthesis Kit (Table 1).
Table 1. EVery EV RNA Isolation and cDNA Synthesis Products
| Cat. # | Product description |
|---|---|
| EVery100B-1 | EVery EV RNA Isolation Kit |
| EVery200B-1 | EVery cDNA Synthesis Kit |
References
How It Works
How It Works
See the quick and easy EVery workflow
The EVery EV RNA Isolation Kit delivers high yields of highly concentrated RNA from already isolated EVs. The column-based workflow is easy to implement and can be completed in less than 30 minutes (Figure 1).
Figure 1. The quick and easy EVery EV RNA Isolation workflow.
Supporting Data
Supporting Data
EVery captures EVerything
To demonstrate the ability of the EVery EV RNA Isolation Kit to capture the full range of RNAs, we used the kit to isolate RNA from 10,000 cells (Figure 2, lane 1), from EVs that were isolated from 250 µL of serum using SmartSEC Single (Figure 2, lane 2), and from buffer spiked with 0.1 pmol of Cel-miR-39 (Figure 2, lane 3). The high quality of the isolated RNA can be seen in lane 1, where the RNA integrity number (RIN) is 9.9 and the 28S/18S RNA ratio is 1.5. The multiple bands in lane 2 demonstrate that EVery captures RNAs of different lengths—EVerything—from EVs with no apparent bias or size preference. The strong signal from the spiked-in miRNA in lane 3 demonstrates the good recovery of even small RNAs.
Figure 2. EVery captures EVerything.
The compatibility of multiple EV isolation techniques with EVery and the excellent size distribution of RNAs isolated from those EVs is shown in Figure 3.
Figure 3. EVery is compatible with EVs isolated using ExoQuick Ultra and SmartSEC Single.
EVery delivers similar amounts of RNA as phenol-based methods
To demonstrate the excellent RNA yields obtained with EVery, we isolated EVs from 250 µL of serum using SmartSEC Single, spiked in 0.1 pmol of Cel-miR-39, and used both EVery and a phenol-based kit to isolate RNA. The isolated RNA was reverse transcribed using the EVery cDNA Synthesis Kit and the copy number of Cel-miR-39 measured (Figure 4). TheEVery EV RNA Isolation Kit delivered similar levels of Cel-miR-39 as the phenol-based method.
Figure 4. EVery is EVery bit as good as phenol.
EVery efficiently isolates mRNA
We used the EVery EV RNA Isolation kit to isolate mRNA from cells overexpressing eGFP (Figure 5). Robust levels of eGFP mRNA are recovered when cells are overexpressing eGFP.
Figure 5. EVery efficiently isolates longer RNAs like mRNA.
miRNA isolated using EVery can be used for miRNA profiling and RNA-seq
We isolated EVs from 250 µL of normal or breast cancer patient serum using SmartSEC Single and used EVery EV RNA Isolation Kit and EVery cDNA Synthesis Kit to isolate and reverse transcribe EV RNAs for miRNA profiling using EVery miRNome Profiler for Human Serum and Plasma (EVery500B-1). Distinct miRNA expression profiles of breast cancer patient serum compared to normal serum are shown in Figure 6. Differential expression of selected breast cancer associated miRNA markers were further confirmed by qPCR (Figure 7).
Figure 6. EVery family of products generate high-quality cDNA suitable for miRNA profiling.
Figure 7. Differential expression of selected miRNAs in normal vs. breast cancer serum confirmed by qPCR. Data normalized with global mean. Expression of miR-451a, miR-21-5p, miR-16-5p and miR-103a are significantly upregulated, while expression of the tumor suppressor miR-124-3p is significantly downregulated in breast cancer serum compared to normal serum.
We were also able to show robust, successful RNA-seq runs using RNA isolated from EVs with EVery (Table 2). All three EV isolation methods tested generated high-quality RNA-seq data.
Table 2. Successful RNA-seq with EVery-isolated EV RNA
EV Isolation Method | Amount of RNA isolated (ng) | Number of reads | FAST-QC |
|---|---|---|---|
4.3 | 53,349,528 | Passed |
|
5.4 | 107,154,128 | Passed |
|
5.2 | 98,886,924 | Passed |